Journal
CLINICAL IMMUNOLOGY
Volume 136, Issue 1, Pages 83-95Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2010.03.001
Keywords
Retinal pigment epithelium; Immune privilege; Regulatory T cells; TGF beta; Eye
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Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan [20592073, 21791672]
- Grants-in-Aid for Scientific Research [20592073, 21791672] Funding Source: KAKEN
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Murine retinal pigment epithelial (RPE) cells suppress T-cell activation by releasing soluble inhibitory factors and promote the generation of regulatory T cells in vitro. These T cells exposed to RPE supernatants (RPE-induced Treg cells) can suppress the activation of bystander effector T cells via the production of transforming growth factor-beta (TGF beta). In the present study, we showed that human RPE-induced Treg cells are also able to acquire regulatory function when human RPE cell lines were pretreated with recombinant TGF beta 2. These RPE-induced Treg cells produced TGF beta 1 and IL-10 but not IFN gamma, and they significantly suppressed the activation of target cell lines and intraocular T-cell clones established from patients with active uveitis. Moreover, CD4(+)CD25(+) RPE-induced Treg cells expressed CTLA-4 and Foxp3 molecules, and the CD25(+) Treg cells profoundly suppressed the T-cell activation. Thus, in vitro manipulated Treg cells acquire functions that participate in the establishment of immune tolerance in the eye. (C) 2010 Elsevier Inc. All rights reserved.
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