Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Jianlei Shen, Bo Situ, Xianchao Du, Zhiming Wang, Rong Hu, Baixue Li, Anjun Qin, Ben Zhong Tang
Summary: This study presents a strategy to improve the detection sensitivity and broaden the dynamic range of enzyme-linked immunosorbent assay (ELISA) by replacing traditional chromogenic reagents with an aggregation-induced emission luminogen (AIEgen). The developed AIE-ELISA showed a lower limit of detection and a broader dynamic range compared to single-mode ELISA in the detection of prostate-specific antigen (PSA), allowing for differentiation of minor PSA differences in patient's serum. The AIE-ELISA demonstrated simpler experimental operation, faster enzyme response speed, and better photostability than traditional chromogenic reagents, making it highly promising in the fields of immunoassay, immunohistochemistry, and immunocytochemistry.
Article
Chemistry, Analytical
Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Yunjing Li, Gang Wu, Jingang Liang, Hongfei Gao
Summary: This study developed a specific and sensitive detection method for the cauliflower mosaic virus 35s (CaMV35S) promoter in genetically modified (GM) crops using the nuclease-dead Cas9 (dCas9)/sgRNA system and a clustered regularly interspaced short palindromic repeats (CRISPR)/dCas9-mediated enzyme-linked immunosorbent assay (ELISA). The proposed method offers a specific, sensitive, rapid, and cost-effective detection platform for molecular diagnoses.
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Analytical
Hinata Maeno, Satoshi Ogata, Tetsuhide Shimizu, Ming Yang
Summary: The authors have developed a film-stacked structure using polyethylene terephthalate (PET) films, which can be combined with 96-well microplates for biochemical analysis. By inserting this structure into a well and rotating it, convection flow is generated to enhance chemical reactions between molecules. However, only a part of the solution circulates into the narrow gaps between the films, resulting in lower reaction efficiency. In this study, unsteady rotation is applied to improve analyte transport into the gaps using secondary flow generated on the rotating disk surface. Finite element analysis is used to optimize the rotation conditions and evaluate the molecular binding ratio.
Article
Plant Sciences
Lucia Vieira Hoffmann, Amanda Alves Branquinho, Paulo Augusto Vianna Barroso, Maite F. S. Vaslin
Summary: By developing monoclonal and polyclonal antibodies based on coat protein peptides, an efficient ELISA detection method was established to detect the cotton blue disease virus in cotton and weeds. The study has the potential to guide the development of other diagnostic procedures.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Parasitology
Phattharaphon Wongphutorn, Chanika Worasith, Kulthida Y. Kopolrat, Chutima Homwong, Jiraporn Sithithaworn, Chatanun Eamudomkarn, Anchalee Techasen, Patcharaporn Tippayawat, Opal Pitaksakurat, Nuttanan Hongsrichan, Thomas Crellen, Paiboon Sithithaworn
Summary: Detection of specific IgG4 in urine can achieve similar diagnostic accuracy to serum for strongyloidiasis. This suggests that urine IgG4 can be used as a valid diagnostic marker.
PARASITES & VECTORS
(2023)
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Agriculture, Dairy & Animal Science
Aicha Yasmine Bellatreche, Riad Bouzid, Amandine Blaizot, Dominique Aubert, Radu Blaga, Khatima Ait-Oudhia, Delphine Le Roux
Summary: Toxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii, affecting most warm-blooded animals. Assessing the seroprevalence of T. gondii in different animal species gives a good estimate of the global circulation of the parasite and the risk for human infections. This study compared two methods for detecting T. gondii antibodies in hunting dogs and found a high prevalence of infection. It emphasizes the importance of studying T. gondii infections in companion animals to assess the risk for human populations.
Article
Biotechnology & Applied Microbiology
Xinyang Li, Miyang Li, Shengbao Duan, Hongmei Wang, Yong Li, Tiemei Liu
Summary: The objective of this study is to develop a simple, rapid, reliable, sensitive, and cost-effective method for prenatal detection of fetomaternal haemorrhage by combining a multi-aperture silk membrane with enzyme-linked immunosorbent assay (ELISA). This method does not require any complicated instruments and can be visually colored, providing a new method for clinical detection of fetomaternal haemorrhage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Critical Care Medicine
Charlotte Oris, Julie Durif, Marion Rouzaire, Bruno Pereira, Damien Bouvier, Samy Kahouadji, Mathieu Abbot, Marina Brailova, Sylvain Lehmann, Christophe Hirtz, Philippe Decq, Bernard Dusfour, Nicola Marchi, Vincent Sapin
Summary: This study evaluated the use of seven blood biomarkers for sport-related concussions. Only S100B and GFAP remained stable over the course of a season. The individual percentage change of S100B and NFL concentrations measured 36 hours after concussion was significantly different between non-resolutive and resolutive cases.
JOURNAL OF NEUROTRAUMA
(2023)
Article
Biochemistry & Molecular Biology
Nicolas Tessandier, Ilkay Basak Uysal, Baptiste Elie, Christian Selinger, Claire Bernat, Vanina Boue, Sophie Grasset, Soraya Groc, Massilva Rahmoun, Bastien Reyne, Noemi Bender, Marine Bonneau, Christelle Graf, Vincent Tribout, Vincent Foulongne, Jacques Ravel, Tim Waterboer, Christophe Hirtz, Ignacio G. Bravo, Jacques Reynes, Michel Segondy, Carmen Lia Murall, Nathalie Boulle, Tsukushi Kamiya, Samuel Alizon
Summary: The study examines the association between the use of different types of menstrual products and microbial, immunological, demographic, and behavioral indicators. The results suggest a potential link between the use of menstrual cups and fungal genital infection, highlighting the possible influence of menstrual products on menstrual health.
Review
Clinical Neurology
C. Delaby, C. Hirtz, S. Lehmann
Summary: The increasing number of people with advanced Alzheimer's disease (AD) is a significant burden to the world population. Accurate detection of the earliest phase of preclinical AD is crucial for prevention and therapy. Blood biomarkers have the potential for early and minimally invasive detection of AD, as well as differential diagnosis of dementia and disease monitoring.
REVUE NEUROLOGIQUE
(2023)
Article
Medical Laboratory Technology
Chiara Giangrande, Helene Vaneeckhoutte, Amandine Boeuf, Beatrice Lalere, Christophe Hirtz, Sylvain Lehmann, Milena Quaglia, Vincent Delatour
Summary: The objective of this work is to develop a higher order candidate reference measurement procedure (RMP) for the absolute quantification of t-tau in cerebrospinal fluid. A primary calibrator consisting of a highly purified recombinant protein was sourced to establish metrological traceability. An isotopically-labelled homologue was obtained to develop a candidate RMP by isotope dilution mass spectrometry (IDMS) for t-tau absolute quantification. The candidate RMP has been validated and can be used for global standardization of CSF t-tau measurements.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Clinical Neurology
Mehdi Brousse, Constance Delaby, Elisa De La Cruz, Jens Kuhle, Pascal Benkert, Etienne Mondesert, Nelly Ginestet, Christophe Hirtz, William Camu, Sylvain Lehmann, Florence Esselin
Summary: The study compared the diagnostic performances of two ultrasensitive methods (Ella and Simoa) for the measurement of the neurofilament light chain (NfL) in patients with amyotrophic lateral sclerosis (ALS). Comparable results were obtained from both methods, and cut-off values were proposed for the clinical use of this biomarker.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jerome Vialaret, Margaux Vignon, Stephanie Badiou, Gregory Baptista, Laura Fichter, Anne-Marie Dupuy, Aleksandra Maleska Maceski, Martin Fayolle, Mehdi Brousse, Jean-Paul Cristol, Claude Jeandel, Christophe Hirtz, Sylvain Lehmann
Summary: Blood microsampling combined with large panels of clinically relevant tests is important for the development of home sampling and predictive medicine. This study aimed to demonstrate the practicality and medical utility of microsamples quantification using mass spectrometry (MS) in a clinical setting. The analysis of microsamples allowed the quantification of 62 proteins, with 48 proteins significantly correlated between microsampling plasma and dried blood spot (DBS). This type of analysis opens new perspectives in diagnosis, monitoring, and risk assessment for personalized medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Sylvain Lehmann, Susanna Schraen-Maschke, Jean-Sebastien Vidal, Constance Delaby, Frederic Blanc, Claire Paquet, Bernadette Allinquant, Stephanie Bombois, Audrey Gabelle, Olivier Hanon
Summary: Plasma P-tau181 is a diagnostic marker for Alzheimer's disease that effectively detects A beta+ status and conversion to dementia. However, its levels can be influenced by renal function and should be taken into account in the diagnosis.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Margaux Vignon, Amandine Bastide, Aurore Attina, Alexandre David, Philippe Bousquet, Valerie Orti, Jerome Vialaret, Sylvain Lehmann, Dominique DevilleDe Periere, Christophe Hirtz
Summary: This study used multiplexed mass spectrometry (MS) to analyze the salivary epitranscriptomic profiles as periodontitis biomarkers. Some nucleosides were found to be significantly altered in periodontitis patients, which may serve as promising biomarkers. This research provides new directions and insights for the discovery of diagnostic biomarkers for periodontitis.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Medical Laboratory Technology
Sylvain Lehmann, Susanna Schraen-Maschke, Jean-Sebastien Vidal, Constance Delaby, Frederic Blanc, Claire Paquet, Bernadette Allinquant, Stephanie Bombois, Audrey Gabelle, Olivier Hanon, BALTAZAR Study Grp
Summary: This study compared the clinical performance of Lumipulse and Simoa assays in predicting the conversion to dementia in patients with mild cognitive impairment (MCI). The results showed that both assays had similar abilities, but Lumipulse was less affected by comorbidities. Plasma pTau181 was associated with renal dysfunction, cardiovascular factors, nutrition, and inflammatory markers. The study suggests that Lumipulse could be used as a noninvasive diagnostic tool for the management of Alzheimer's disease.
CLINICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Salome Coppens, Sylvain Lehmann, Christopher Hopley, Christophe Hirtz
Summary: Neurofilament-light chain (Nf-L) is a widely studied biomarker for neurodegenerative diseases (NDD) and traumatic brain injuries (TBI). It can now be measured in blood using ultrasensitive assays, but the development of reliable clinical thresholds and reference measurement procedures (RMP) is crucial for successful clinical implementation. The use of tools like mass spectrometry is ongoing to establish RMP and define the measurand for standardization. This article summarizes the current methods for Nf-L quantification and discusses the progress and challenges in developing RMP and defining the measurand for diagnostic standardization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Jerome Vialaret, Margaux Vignon, Christophe Hirtz, Stephanie Badiou, Gregory Baptista, Laura Fichter, Anne-Marie Dupuy, Aleksandra Maleska Maceski, Martin Fayolle, Mehdi Brousse, Jean-Paul Cristol, Claude Jeandel, Sylvain Lehmann
Summary: This study aimed to evaluate the feasibility of using mass spectrometry on dried blood spots (DBS) in a clinical setting. The results showed that mass spectrometry is a reliable method for quantifying protein biomarkers related to nutrition and inflammation. However, there was no correlation between these proteins and patient frailty.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
