Journal
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Volume 50, Issue 3, Pages 573-581Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/CCLM.2011.769
Keywords
breath analysis; chemometrics; gas chromatography and mass spectrometry (GC/MS); lung cancer biomarkers; solid-phase microextraction (SPME)
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Funding
- Polish Ministry of Sciences and High Education [NN204 322137, NN204026238]
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Background: Volatile organic compounds (VOCs) present in breath of lung cancer patients and released by lung cancer cells were determined. As a reference sample breath collected from healthy volunteers and lung segments of cells with proper morphology were used. Methods: Solid phase microextraction and gas chromatography mass spectrometry (SPME-GC/MS) method was applied for VOC analysis. Statistical methods have been developed to find similarities between patterns of investigated compounds within three populations of samples: exhaled air samples from patients with lung cancer, headspace of lung tissues with healthy and cancer cells. Results: Quantitative analysis of VOCs in the headspace of lung :issues revealed that cancer cells released higher concentrations of ethanol, acetone, carbon disulfide, dimethyl sulfide, 1-propanol, 2-propanol, 2-butanone and 2-pentanone than healthy tissues. The increase of concentration of the same compounds was observed in the breath of patients with lung cancer in comparison to breath from healthy non-smoking volunteers. Conclusions: Detection of lung cancer is possible by volatile biomarkers analysis in breath.
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