Article
Microbiology
Annelieke Overbeeke, Bela Hausmann, Georgi Nikolov, Fatima C. Pereira, Craig W. Herbold, David Berry
Summary: The study found that not all gut bacteria capable of degrading chondroitin sulfate could also degrade hyaluronan, despite having lyases that act on both compounds. In the model organism Bacteroides, a lyase along with surface binding proteins may be involved in hyaluronan breakdown, and degradation of these compounds provides public goods for other bacteria. Cooperative degradation and cross-feeding may be widespread in mucosal glycan utilization.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Litao Hu, Yang Wang, Yunxiao Hu, Juyi Yin, Lingling Wang, Guocheng Du, Jian Chen, Zhen Kang
Summary: A membrane shield strategy was developed to produce high-molecular-weight glycosaminoglycans by recruiting type II NSGAG synthases in Corynebacterium glutamicum, while a synchronized depolymerization-polymerization strategy was developed by coexpressing NSGAGs synthases and lyases for specific size oligosaccharides production. These strategies have significant implications for the field of biomaterials.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Shinichiro Suto, Ikuko Kakizaki, Yota Tatara, Masahiko Endo
Summary: The structure of hyaluronan is crucial for binding to HABP, with particular emphasis on the non-reducing terminal structure. Replacement of internal disaccharide units is possible for chains decasaccharide or larger.
CARBOHYDRATE POLYMERS
(2021)
Review
Biochemistry & Molecular Biology
Annika Wartenberg, Juergen Weisser, Matthias Schnabelrauch
Summary: This review presents the latest advances in cryogels containing mainly glycosaminoglycans (GAGs) or composites of GAGs and other natural or synthetic polymers. These cryogels have a structure resembling the native extracellular matrix and show excellent biocompatibility, making them suitable for applications in tissue regeneration and tissue engineering with flexibility in design and injectability.
Article
Biochemistry & Molecular Biology
Jonas Knittel, Nadja Itani, Rolf Schreckenberg, Jacqueline Heger, Susanne Rohrbach, Rainer Schulz, Klaus-Dieter Schlueter
Summary: Serotonin effects on cardiac hypertrophy, senescence, and failure depend on specific receptors or serotonin uptake and degradation by MAOs. MAO-dependent effects are non-specific. MAOA expression increases in maladaptive hypertrophic stages. MAO-A inhibition suppresses serotonin-dependent damage. Serotonin induces mitochondria-dependent oxidative stress.
Review
Biochemistry & Molecular Biology
Aikaterini Berdiaki, Monica Neagu, Eirini-Maria Giatagana, Andrey Kuskov, Aristidis M. Tsatsakis, George N. Tzanakakis, Dragana Nikitovic
Summary: The tumor microenvironment (TME) is composed of various cells and extracellular matrix components. Changes in the structure and sulfation pattern of GAGs are associated with cancer progression. GAGs and GAG-based molecules show promise as potential effectors for anticancer therapy due to their unique properties.
Article
Materials Science, Multidisciplinary
Rui R. Costa, David Caballero, Diana Soares da Costa, Romen Rodriguez-Trujillo, Subhas C. Kundu, Rui L. Reis, Iva Pashkuleva
Summary: Anisotropy plays a crucial role in the organization and healing of neural tissues, and holds great potential for developing regeneration strategies. The study demonstrates the fabrication of microfibers from charged ECM components using interfacial polyelectrolyte complexation, which can mimic the mechanical properties of neural tissues. These fibers are biocompatible and promote the formation of neural processes, enabling the development of neural cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biochemistry & Molecular Biology
Richard Kehm, Tim Baldensperger, Jana Raupbach, Annika Hoehn
Summary: Generation of reactive oxygen species and related oxidants during life processes can lead to irreversible modifications of proteins, potentially contributing to pathological conditions and diseases.
Article
Agriculture, Dairy & Animal Science
Carl A. Frame, Elisabeth Huff-Lonergan, Brian J. Kerr, Mariana Rossoni Serao
Summary: The study aimed to investigate the effects of feedstuff containing oxidized protein and lipid on diet digestibility, growth performance, and oxidative stress in nursery pigs. Results showed that feeding pigs with oxidized proteins and lipids reduced feed intake and growth performance, but did not induce oxidative stress.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Jura-Poltorak, Pawel Olczyk, Aleksandra Chalas-Lipka, Katarzyna Komosinska-Vassev, Kornelia Kuznik-Trocha, Katarzyna Winsz-Szczotka, Diana Ivanova, Yoana Kiselova-Kaneva, Katarzyna Krysik, Alicja Telega, Krystyna Olczyk
Summary: This study analyzed the pattern of urinary excretion of total sulphated glycosaminoglycans (GAGs) and their particular types in obese patients with type 2 diabetes mellitus (T2DM) treated with metformin. The results showed that metformin therapy for six months significantly decreased the urinary excretion of total GAGs, CS/DS, and HS.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Vy Ngo, Nadun C. Karunatilleke, Anne Brickenden, Wing-Yiu Choy, Martin L. Duennwald
Summary: Cells respond to oxidative stress by inducing antioxidant proteins through the activation of Nrf2. Both Nrf2 and Keap1 are susceptible to protein misfolding under oxidative stress, potentially due to their intrinsic characteristics. This study provides new insights into the regulation of Nrf2 and Keap1 by oxidation-induced protein misfolding.
Article
Biochemistry & Molecular Biology
Katerina Stepankova, Dana Marekova, Kristyna Kubasova, Radek Sedlacek, Karolina Turnovcova, Irena Vackova, Sarka Kubinova, Pavol Makovicky, Michaela Petrovicova, Jessica C. F. Kwok, Pavla Jendelova, Lucia Machova Urdzikova
Summary: This study evaluated the physiological changes and potential side effects of 4-methylumbelliferone (4MU) treatment in healthy rats. The results showed increased bile acids, blood sugars, and proteins after 4MU administration, as well as increased interleukins IL10, IL12p70, and IFN gamma. However, these effects were reversed after a 9-week wash-out period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Lynn Kruger, Karina Biskup, Vasileios Karampelas, Antje Ludwig, Antje-Susanne Kasper, Wolfram C. Poller, Veronique Blanchard
Summary: In this study, an optimized strategy was presented to analyze glycosaminoglycan disaccharides via MALDI-TOF mass spectrometry, successfully identifying sulfation positions and patterns through fragmentation experiments.
Article
Pharmacology & Pharmacy
Elizabeth M. Arrigali, Monica A. Serban
Summary: The covalent conjugate of hyaluronan with d-Methionine shows prophylactic potential against oxidative damage and is suitable for topical delivery. It demonstrates cytocompatibility and protection from oxidative stress in two mouse cochlear cell lines through hyaluronan-mediated cellular internalization of the antioxidant. The conjugate can efficiently permeate through an in vitro round window membrane model without losing the attached antioxidant, suggesting its potential as a topical preventive agent against hearing loss.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Cornelia Zapp, Patricia Mundinger, Heike Boehm
Summary: Glycosaminoglycans (GAGs) are essential in tissue homeostasis, and GAG-based biomaterials play a significant role in tissue engineering. Current biomaterials use crosslinks between GAG chains, but immobilizing GAGs onto polyethylene glycol (PEG) hydrogels can create a natural presentation of GAGs with finely-tunable mechanobiochemical properties.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Jura-Poltorak, Pawel Olczyk, Aleksandra Chalas-Lipka, Katarzyna Komosinska-Vassev, Kornelia Kuznik-Trocha, Katarzyna Winsz-Szczotka, Diana Ivanova, Yoana Kiselova-Kaneva, Katarzyna Krysik, Alicja Telega, Krystyna Olczyk
Summary: This study analyzed the pattern of urinary excretion of total sulphated glycosaminoglycans (GAGs) and their particular types in obese patients with type 2 diabetes mellitus (T2DM) treated with metformin. The results showed that metformin therapy for six months significantly decreased the urinary excretion of total GAGs, CS/DS, and HS.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Medicine, General & Internal
Kornelia Kuznik-Trocha, Katarzyna Winsz-Szczotka, Iwona Lachor-Motyka, Klaudia Dabkowska, Magdalena Wojdas, Krystyna Olczyk, Katarzyna Komosinska-Vassev
Summary: This study assessed the effects of 24-month anti-TNF-alpha treatment on the remodeling of cartilage extracellular matrix in patients with juvenile idiopathic arthritis. The results showed that the therapy improved the levels of hyaluronic acid, but did not completely regenerate the cartilage. Changes in plasma hyaluronic acid levels may be used as a potential diagnostic tool for monitoring disease activity and assessing treatment efficacy.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Wojdas, Klaudia Dabkowska, Kornelia Kuznik-Trocha, Grzegorz Wisowski, Iwona Lachor-Motyka, Katarzyna Komosinska-Vassev, Krystyna Olczyk, Katarzyna Winsz-Szczotka
Summary: This study assessed the effects of ETA therapy on ECM metabolism in JIA patients. The results showed that GAGs and TAS concentrations were significantly lower in patients with aggressive JIA, while MMP-1 and MMP-3 levels were significantly higher. Anti-cytokine therapy did not normalize any of the assessed parameters.
Article
Medicine, General & Internal
Klaudia Dabkowska, Magdalena Wojdas, Kornelia Kuznik-Trocha, Grzegorz Wisowski, Anna Gruenpeter, Katarzyna Komosinska-Vassev, Krystyna Olczyk, Katarzyna Winsz-Szczotka
Summary: The levels of galactosaminoglycans (GAAGs) and oligomeric cartilage matrix protein (COMP) are significantly decreased, while the levels of human cartilage glycoprotein 39 (YKL-40) are increased in juvenile idiopathic arthritis (JIA) patients, which can serve as potential biomarkers for assessing the efficacy of biologic therapy. Treatment with etanercept (ETA) can normalize the levels of COMP and YKL-40, but does not significantly affect the levels of GAAGs. ADAMTS4, ADAMTS5, and PDGF-BB have significant influences on the levels of these markers.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yoana Kiselova-Kaneva, Milka Nashar, Bogdan Roussev, Ayshe Salim, Minka Hristova, Pawel Olczyk, Katarzyna Komosinska-Vassev, Ivayla Dincheva, Ilian Badjakov, Bistra Galunska, Diana Ivanova
Summary: This study evaluated the immunomodulatory potential of Sambucus ebulus fruit infusion in healthy humans. The results showed that the infusion decreased the levels of pro-inflammatory markers and complement activity, indicating its immune-modulatory activity in healthy individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Merve Keskin, Gulsen Kaya, Sinan Bayram, Anna Kurek-Gorecka, Pawel Olczyk
Summary: In this study, chestnut honey-based silver nanoparticles were synthesized at different temperatures and characterized using various techniques. The nanoparticles displayed antioxidant activity and inhibition effects on important enzymes. They also exhibited antibacterial activity against both Gram-positive and Gram-negative bacteria, suggesting their potential applications in medicine, pharmacy, and cosmetic technology.
Review
Cell Biology
Sebastian Stepien, Pawel Olczyk, Joanna Gola, Katarzyna Komosinska-Vassev, Aleksandra Mielczarek-Palacz
Summary: Due to their multidirectional influence, adipocytokines are currently the subject of numerous intensive studies. Significant impact applies to many processes, both physiological and pathological. Moreover, the role of adipocytokines in carcinogenesis seems particularly interesting and not fully understood. Ongoing research focuses on the role of these compounds in the network of interactions in the tumor microenvironment, with special attention on ovarian and endometrial cancer, and their potential clinical relevance.
Article
Pharmacology & Pharmacy
Anna Kurek-Gorecka, Pawel Ramos, Malgorzata Klosek, Elzbieta Bobela, Zenon P. Czuba, Radoslaw Balwierz, Pawel Olczyk
Summary: Propolis is a source of compounds with strong antibacterial activity. The aim of the study was to evaluate the antibacterial effect of Polish propolis against cariogenic bacteria. The results suggest that propolis may be beneficial in preventing dental plaque and caries.
Article
Medicine, General & Internal
Anna Szeremeta, Agnieszka Jura-Poltorak, Aleksandra Zon-Giebel, Krystyna Olczyk, Katarzyna Komosinska-Vassev
Summary: Tumor necrosis factor alpha inhibitor (TNF alpha I) therapy, combined with methotrexate, can effectively inhibit radiographic progression and improve physical function and quality of life among patients with rheumatoid arthritis (RA). The study evaluated the effect of 15-month anti-TNF-alpha therapy on cartilage turnover markers in female RA patients and found significant changes in serum levels of biochemical markers and ratios. Baseline COMP levels were associated with disease activity and could be used as a predictor. The C2C/PIICP ratio showed promising potential as a biomarker for monitoring the effectiveness of anti-TNF-alpha treatment.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Kaluzna, Agnieszka Jura-Poltorak, Alicja Derkacz, Krystyna Olczyk, Katarzyna Komosinska-Vassev
Summary: This case-control study aimed to identify novel biomarkers for Crohn's disease (CD) and found significant differences in serum concentrations of proguanylin (pro-GN), pentraxin 3 (PTX3), and S100A12 between CD patients and healthy individuals. The serum profile of PTX3 was also correlated with disease activity and treatment response. These findings suggest that measuring pro-GN, PTX3, and S100A12 could be beneficial for the diagnosis and monitoring of CD.
Review
Biochemistry & Molecular Biology
Anna Kurek-Gorecka, Karolina Walczynska-Dragon, Rafael Felitti, Stefan Baron, Pawel Olczyk
Summary: Oral health is related to systemic diseases. Propolis and a diet rich in polyphenols can reduce dental plaque accumulation, decrease the severity of COVID-19, and play an important role in preventing systemic diseases.