Review
Biochemistry & Molecular Biology
Fatima Hameedat, Sahar Hawamdeh, Soraya Alnabulsi, Aref Zayed
Summary: This review presents the HPLC-FLD methods reported in the literature for the quantification of steroids in clinical, pharmaceutical, and environmental applications, with a focus on the various approaches of fluorescent derivatization.
Article
Biochemistry & Molecular Biology
Maria S. Synaridou, Vasilis Tsamis, Georgia Sidiropoulou, Constantinos K. Zacharis, Irene Panderi, Catherine K. Markopoulou
Summary: Amino acids have ergogenic effects on young athletes by enhancing, protecting, and restoring their muscular system. Encapsulating five relevant amino acids in chocolate pellets enhances daily intake among them. A reliable HPLC fluorimetric method was developed to detect and quantitatively determine amino acids in chocolate, with experimental design methodology used to optimize the clean-up procedure.
Article
Environmental Sciences
Lin Chen, Shun Kong, Guodong Wang, Xiaoju Yan, Xuemei Zhang, Xiangji Kong, Yuanqing Bu
Summary: This study developed an analytical method using reversed-phase high-performance liquid chromatography to quantify glufosinate-P-ammonium (GLUF-P) in farmland soil. The method showed high sensitivity and could be used for the determination of GLUF-P residues in farmland soil.
Review
Chemistry, Analytical
Raghav Dogra, Mohit Kumar, Arvind Kumar, Marco Roverso, Sara Bogialli, Paolo Pastore, Uttam Kumar Mandal
Summary: Derivatization is a valuable practice for analyzing difficult analytes in complex matrices. The availability of derivatizing reagents has made analyte modification more feasible. Derivatization techniques have enhanced the analytical strength in recent years. Although conventional HPLC lacks sufficient sensitivity, combining it with derivatization can significantly improve sensitivity.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qiao-Lian Duan, Hai-Wei Shi, Li Tan, Zhen Liu, Qing Huang, Wei Shen, Ling Cao, Hian Kee Lee, Sheng Tang
Summary: A new ultrahigh-performance supercritical fluid chromatography (UHPSFC) method was developed to solve the difficult separation problem of various biogenic amines (BAs) in gentamicin. The method achieved complete separation of 10 BAs using dansyl chloride derivatization and UHPSFC system. It demonstrated high sensitivity and accuracy, and met the quantification limits required for impurity determination.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Corey W. W. Manwaring, Jake A. A. Cravino, Margi Patel, Jonathan G. H. Stathakis, Arianne Soliven, Thirada Suktham, Ross Andrew Shalliker
Summary: Coffee is a popular beverage, with a growing market worth over USD 4 billion. Coffee fraud is a concerning issue, with up to one in five coffees being contaminated with cheaper blends. Climate change and the recent Brazil frost have further impacted coffee production, leading to a potential increase in coffee fraud. In this study, a novel approach using postcolumn derivatization is proposed to authenticate coffee samples in real-time, along with three mathematical similarity metrics.
Article
Food Science & Technology
Nor Shifa Shuib, Bahruddin Saad
Summary: A new sample pretreatment technique, ISD mu SPE, coupled with high performance liquid chromatography, was used for simultaneous determination of aflatoxins in milk. Online photo-chemical derivatization and optimization of parameters led to high sensitivity and accuracy in the analysis.
Review
Chemistry, Analytical
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: Glutathione, as the most abundant antioxidant in living organisms, plays a crucial role in cells due to its free thiol group. Its determination using liquid chromatography has been widely studied and proves significant in understanding the development of human diseases.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Marzena Pawul-Gruba, Tomasz Kiljanek, Anna Madejska, Jacek Osek
Summary: A method for determining eight biogenic amines in ripened cheeses was developed and validated using HPLC-DAD, showing high accuracy and precision.
Article
Biochemistry & Molecular Biology
Yun Ai, Yan Ni Sun, Li Liu, Fang Yuan Yao, Yan Zhang, Feng Yi Guo, Wen Jie Zhao, Jian Li Liu, Ning Zhang
Summary: This study successfully characterized biogenic amines in different parts of Lycium barbarum L. using HPLC, revealing four different types of amines. The bark had the highest histamine content, while the flower had the highest putrescine content. There were significant differences in biogenic amine content among different parts of Lycium barbarum L., which should be considered when further utilizing them.
Article
Biochemistry & Molecular Biology
Marianna Ntorkou, Eleni Tsanaktsidou, Konstantina Chachlioutaki, Dimitrios G. Fatouros, Catherine K. Markopoulou
Summary: Homotaurine shows promising potential as a drug for treating neurodegenerative diseases such as Alzheimer's. The study investigates the permeability of homotaurine in vitro and finds that it exhibits superior permeability through the simulated blood-brain barrier compared to memantine, suggesting potential for enhanced drug delivery strategies targeting neurological conditions.
Article
Biochemistry & Molecular Biology
Yue Wang, Jing Feng, Song Wu, Huihui Shao, Wenxuan Zhang, Kun Zhang, Hanyilan Zhang, Qingyun Yang
Summary: A new method using derivatization high-performance liquid chromatography with ultraviolet detection was developed and validated for the quantitative analysis of genotoxic impurities in a novel drug. The method was found to be reliable and practical through optimization and validation of key experimental parameters.
Article
Biochemistry & Molecular Biology
Meg Shieh, Shi Xu, Oren L. Lederberg, Ming Xian
Summary: This review summarizes current detection methods for sulfane sulfur species, including cyanolysis, chemical derivatization and mass spectrometry, proteomic analysis, fluorescent probes, and resonance synchronous/Raman spectroscopic methods. The design principles, advantages, applications, and limitations of each method are discussed, along with suggested directions for future research on these methods.
Article
Chemistry, Applied
Qianyu Li, Guoliang Li, Lihua Fan, Yanxin Yu, Jin Liu
Summary: A promising click fluorescent labeling method with high selectivity for the determination of five steroid hormones in food samples was proposed. The method relies on the reaction between 3-Azido-7-hydroxycoumarin and the alkynyl group of steroid hormones in the presence of copper (I), resulting in a turn-on fluorescence response. The method showed excellent performance with good linearity and low detection limit when detected by HPLC-FLD.
Article
Biochemical Research Methods
Martin Braun, Sarka Ryglova, Tomas Suchy
Summary: This study presents a sensitive reversed-phase gradient HPLC method combined with fluorescence detection for quantifying glycosaminoglycans (GAGs) in collagen-based biomaterials. The method allows for the identification and separation of GAGs from other components with a low detection limit, making it suitable for laboratory analysis.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
