Journal
CLINICAL CHEMISTRY
Volume 55, Issue 7, Pages 1380-1388Publisher
AMER ASSOC CLINICAL CHEMISTRY
DOI: 10.1373/clinchem.2008.118752
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Funding
- National Center for Research Resources [K23 RR16524, M01-RR-023942-01]
- NIH GCRC [MO1-RR-023942]
- Applied Biosystems/Sciex
- O.P. Soldin [U10HD047890-S]
- NIH/NICHD Obstetrics-Fetal Pharmacology Research Unit (OPRU) Network
- Office of Research on Women's Health
- General Clinical Research Center at Georgetown University
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BACKGROUND: Accurate measurement of free thyroid hormones is important for managing thyroid disorders. Ultrafiltration liquid chromatography tandem mass spectrometry (LC-MS/MS) can reliably measure the concentrations of small molecules, including thyroid hormones. Our study was designed to compare free thyroid hormone measurements performed with immunoassay and LC-MS/MS. METHODS: We studied the performance of LC-MS/MS in 4 different populations comprising pediatric patients, euthyroid adults, and healthy nonpregnant and pregnant women. The samples obtained from each population numbered 38, 200, 28, and 128, respectively. Free thyroxine, free triiodothyronine, and thyroid-stimulating hormone (TSH) concentrations were documented. RESULTS: LC-MS/MS measurement of free thyroid hormones provided better correlation with log-transformed serum TSH in each population and also the populations combined. The correlations between free thyroxine measured by LC-MS/MS and log TSH in the pediatric outpatients and healthy adults were -0.90 and -0.77, respectively. The correlations for immunoassay were -0.82 and -0.48. The correlations between free triiodothyronine measured by LC-MS/MS and TSH for both pediatric and healthy adult populations were -0.72 and -0.68, respectively. CONCLUSIONS: Free thyroid hormone concentrations measured by LC-MS/MS correlate to a greater degree with log TSH values compared to concentrations measured by immunoassay. This correlation was maintained across the patient populations we studied and may reflect the accuracy and specificity of LC-MS/MS. The superior ability of LC-MS/MS to enable documentation of the well-known thyroid hormone-TSH relationship supports the use of this measurement technique in a variety of clinical situations. (C) 2009 American Association for Clinical Chemistry
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