Journal
CLINICAL CHEMISTRY
Volume 54, Issue 3, Pages 534-541Publisher
AMER ASSOC CLINICAL CHEMISTRY
DOI: 10.1373/clinchem.2007.098368
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BACKGROUND: Patients with inflammatory bowel disease (IBD) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies (pANCA). a-Enolase has been proposed as a target antigen in IBD. We evaluated the prevalence and diagnostic value of anti-pANCA-enolase antibodies in IBD and related disorders. METHODS: We used a classic proteomic approach with extracts from granulocytes and pANCA-positive ulcerative colitis (UC) sera to confirm a-enolase as a target antigen. By means of Western blot analysis, we screened a cohort of 525 subjects for the presence of anti-alpha-enolase antibodies. We performed GeneArray experiments on RNA extracted from colonic mucosal biopsies from 35 IBD and 6 control patients. RESULTS: We detected anti-a-enolase antibodies 49.0% of patients with UC, 50.0% of patients with Crohn's disease, 30.5% of patients with primary sclerosing cholangitis, 37.8% of patients with autoimmune hepatitis, 34.0% of patients with ANCA-positive vasculitis, 31.0% of non-IBD gastrointestinal controls, and 8.5% of healthy controls. Gene array experiments showed a significant upregulation of alpha-enolase mRNA in colonic mucosal biopsies from patients with IBD, but not from controls. There was no association between the presence of pANCA and anti-alpha-enolase antibodies. Preabsorption with a-enolase did not eliminate the pANCA pattern on indirect immunofluorescence. CONCLUSIONS: Anti-a-enolase antibodies are present in a substantial proportion of patients with IBD, patients with various inflammatory/autoimmune disorders, and non-IBD gastrointestinal controls. Therefore, anti-a-enolase antibodies are of limited diagnostic value for the diagnosis of IBD. (c) 2008 American Association for Clinical Chemistry.
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