Article
Medical Laboratory Technology
Julien Favresse, Johan Schiettecatte, Fleur Wolff, Frederic Cotton, Marc Elsen, Christine Eucher, Catherine Rosseels, Ellen Anckaert
Summary: The performance of the Roche Elecsys Vitamin D total III assay for measuring total 25(OH)D was evaluated in this study. The results showed good agreement with liquid chromatography tandem mass spectrometry, and the assay demonstrated a higher biotin interference threshold and a broader measuring range compared to the previous generation assay. However, there was still an issue with cross-reactivity towards 3-epi-25(OH)D-3 in high titer samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Endocrinology & Metabolism
Lauren A. Burt, Martin Kaufmann, Marianne S. Rose, Glenville Jones, Emma O. Billington, Steven K. Boyd, David A. Hanley
Summary: This randomized controlled trial investigated the effect of high-dose vitamin D-3 on total bone mineral density (TtBMD) in the radius and tibia. The study found that increasing vitamin D dose was associated with increased plasma levels of 25-(OH)D-3, 24,25-(OH)(2)D-3, and 1,24,25-(OH)(3)D-3, but not 1,25-(OH)(2)D-3. These vitamin D metabolites were associated with a decline in TtBMD. The findings suggest that vitamin D metabolites other than 1,25-(OH)(2)D-3 may play a role in bone loss.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Jonas Carneiro Cruz, Israel Donizeti de Souza, Caroline Fernandes Grecco, Eduardo Costa Figueiredo, Maria Eugenia Costa Queiroz
Summary: Bioanalysis faces challenges in sample preparation due to the complexity of biological samples, but recent trends focus on miniaturization, automation, and high-throughput performance. Column switching liquid chromatography, as a multidimensional method, can improve sensitivity and accuracy, reduce organic solvent consumption, and enhance sample handling efficiency.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Biochemistry & Molecular Biology
Hany William Z. Hanna, Cristiano Rizzo, Radwa Marawan Abdel Halim, Hemmat Elewa El Haddad, Randa Salam, Hazem El-Sayed Abou-Youssef
Summary: The study found a stronger association between the FokI AA genotype and HT in Egyptian patients compared to hypothyroid non-HT controls, with patients having FokI AA genotype showing higher levels of 25-OH-vitamin D3 despite normal levels of vitamin D.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Magdalena Zgliczynska, Magdalena Ostrowska, Kinga Zebrowska, Iwona Szymusik, Konrad Kowalski, Dorota Leszczynska, Katarzyna Kosinska-Kaczynska
Summary: This study compared the levels of vitamin D between singleton and twin pregnancies using two diagnostic methods. The results showed no significant difference in the occurrence of vitamin D deficiency between the two groups, but the levels of 25(OH)D were lower in twin pregnancies. The agreement between the two diagnostic methods was low, which has significant clinical implications.
ENDOCRINE CONNECTIONS
(2023)
Article
Nutrition & Dietetics
Cora M. Best, Devon Riley, Thomas J. Laha, Hannah Pflaum, Leila R. Zelnick, Simon Hsu, Kenneth E. Thummel, Karen E. Foster-Schubert, Jessica N. Kuzma, Gail Cromer, Ilona Larson, Derek K. Hagman, Kelly Heshelman, Mario Kratz, Ian H. de Boer, Andrew N. Hoofnagle
Summary: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantification of vitamins D3 and D2 in serum. Results from two pilot clinical trials showed that serum and subcutaneous adipose tissue vitamin D3 concentrations increased proportionally to dose with 3 months of daily supplementation.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2021)
Article
Chemistry, Analytical
R. Rola, K. Kowalski, T. Bienkowski, J. Witwicki, S. Studzinska
Summary: The study demonstrated the utility of DBS samples in assessing newborns' vitamin D levels, comparing them to maternal and cord blood-derived serum tests. Results showed that DBS could be used interchangeably with cord blood-derived serum to determine vitamin D status in neonates, simplifying the screening process.
MICROCHEMICAL JOURNAL
(2021)
Article
Medicine, General & Internal
Huan Li, Xiaomin Xie, Li Zhang, Yanting He, Huili Liu, Dan Qiang, Guirong Bai, Ling Li, Yanpan Tang
Summary: The study found that the disorder of vitamin D metabolism may be related to cholesterol metabolism and bile acid biosynthesis, providing a basis for exploring the possible mechanism leading to abnormal vitamin D metabolism.
Article
Chemistry, Analytical
Jie Zheng, Ge-Ge Gong, Shu-Jian Zheng, Yu Zhang, Yu-Qi Feng
Summary: Carboxylic metabolites, an important class of metabolites in mammals, were successfully labeled using N-methylphenylethylamine (MPEA) to enhance detection sensitivity and chromatographic retention. The optimized method achieved high coverage analysis of carboxylated metabolites in HepG2 cells, identifying a total of 403 potential metabolites.
ANALYTICAL CHEMISTRY
(2021)
Review
Nutrition & Dietetics
Pedro Araujo, Cioly Mendez-Davila
Summary: This article discusses the analytical challenges associated with quantifying vitamin D intake, including studying assay variability before categorizing a method as the golden standard, ensuring sample comparability for meaningful correlations, revising intake guidance based on statistical power analysis, and implementing strategies to prevent preanalytical factors. Addressing these challenges will enable effective vitamin D management in the sports sector.
FRONTIERS IN NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Dae-Hyun Ko, Sun-Hee Jun, Youngwon Nam, Sang H. Song, Minje Han, Yeo-Min Yun, Kyunghoon Lee, Junghan Song
Summary: A multiplex LC-MS/MS method was developed to determine bioavailable vitamin D and VMR, showing differences in bioavailable vitamin D levels among different ethnicities. The new method was robust and reliable, suitable for routine clinical samples.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Cora M. Best, Leila R. Zelnick, Kenneth E. Thummel, Simon Hsu, Christine Limonte, Ravi Thadhani, Howard D. Sesso, JoAnn E. Manson, Julie E. Buring, Samia Mora, I-Min Lee, Nancy R. Cook, Georgina Friedenberg, Heike Luttmann-Gibson, Ian H. de Boer, Andrew N. Hoofnagle
Summary: Vitamin D-3 supplementation increases total serum vitamin D and 25(OH) D concentrations with variation according to baseline 25(OH)D, which suggests that 25-hydroxylation of vitamin D-3 is more efficient when serum 25(OH)D concentration is low.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Engineering, Environmental
Kelly L. Pereira, Martyn W. Ward, John L. Wilkinson, Jonathan Brett Sallach, Daniel J. Bryant, William J. Dixon, Jacqueline F. Hamilton, Alastair C. Lewis
Summary: This study presents an automated method for non-targeted compositional analysis of environmental matrices, which has been rigorously tested for reliability and reproducibility using authentic standards. The method detected over 9600 compounds from critical pollutant sources in environmental samples, demonstrating its ability for rapid and comprehensive compound detection.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Brandon L. Salazar, Zoe Y. Marr, Jason B. Benedict, Luis A. Colon
Summary: Diarylethene-based photoswitches are widely used in chemistry, materials science, and biotechnology due to their unique physical and chemical properties. In this study, a diarylethene-based photoswitchable compound was separated and characterized using high-performance liquid chromatography, and the isomers were purified for individual study. Supercritical fluid chromatography was explored as an alternative separation method, offering faster analysis times and lower solvent consumption compared to high-performance liquid chromatography. This technique could be upscaled for future purification of diarylethene isomeric compounds, providing a more environmentally-friendly approach.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Akiko Nagata, Kazuto Iijima, Ryota Sakamoto, Yuka Mizumoto, Miho Iwaki, Masaki Takiwaki, Yoshikuni Kikutani, Seketsu Fukuzawa, Minami Odagi, Masayuki Tera, Kazuo Nagasawa
Summary: This study describes a versatile method for synthesizing deuterium-labeled D-3 metabolites and successfully applies it to measure the concentration of these compounds in pooled human serum. This includes the first quantification of 1,25(OH)(2)D-3-23,26-lactone in human serum.
Article
Public, Environmental & Occupational Health
Parminder Raina, Nazmul Sohel, Mark Oremus, Harry Shannon, Prem Mony, Rajesh Kumar, Wei Li, Yang Wang, Xingyu Wang, Khalid Yusoff, Rita Yusuf, Romaina Iqbal, Andrzej Szuba, Aytekin Oguz, Annika Rosengren, Annamarie Kruger, Jephat Chifamba, Noushin Mohammadifard, Ebtihal Ahmad Darwish, Gilles Dagenais, Rafael Diaz, Alvaro Avezum, Patricio Lopez-Jaramillo, Pamela Seron, Sumathy Rangarajan, Koon Teo, Salim Yusuf
Article
Nutrition & Dietetics
Humaira Jamshed, Fateh Afi Tipoo Sultan, Romaina Iqba, Anwar Hassan Gilani
JOURNAL OF NUTRITION
(2015)
Article
Medicine, General & Internal
Darryl P. Leong, Koon K. Teo, Sumathy Rangarajan, Patricio Lopez-Jaramillo, Alvaro Avezum, Andres Orlandini, Pamela Seron, Suad H. Ahmed, Annika Rosengren, Roya Kelishadi, Omar Rahman, Sumathi Swaminathan, Romaina Iqbal, Rajeev Gupta, Scott A. Lear, Aytekin Oguz, Khalid Yusoff, Katarzyna Zatonska, Jephat Chifamba, Ehimario Igumbor, Viswanathan Mohan, Ranjit Mohan Anjana, Hongqiu Gu, Wei Li, Salim Yusuf
Correction
Public, Environmental & Occupational Health
Alexandra J. Mayhew, Karen Lock, Roya Kelishadi, Sumathi Swaminathan, Claudia S. Marcilio, Romaina Iqbal, Mahshid Dehghan, Salim Yusuf, Clara K. Chow
PUBLIC HEALTH NUTRITION
(2016)
Article
Endocrinology & Metabolism
Gilles R. Dagenais, Hertzel C. Gerstein, Xiaohe Zhang, Matthew McQueen, Scott Lear, Patricio Lopez-Jaramillo, Viswanathan Mohan, Prem Mony, Rajeev Gupta, V. Raman Kutty, Rajesh Kumar, Omar Rahman, Khalid Yusoff, Katarzyna Zatonska, Aytekin Oguz, Annika Rosengren, Roya Kelishadi, Afzalhussein Yusufali, Rafael Diaz, Alvaro Avezum, Fernando Lanas, Annamarie Kruger, Nasheeta Peer, Jephat Chifamba, Romaina Iqbal, Noorhassim Ismail, Bai Xiulin, Liu Jiankang, Deng Wenqing, Yue Gejie, Sumathy Rangarajan, Koon Teo, Salim Yusuf
Article
Medicine, General & Internal
Andrew Mente, Martin O'Donnell, Sumathy Rangarajan, Gilles Dagenais, Scott Lear, Matthew McQueen, Rafael Diaz, Alvaro Avezum, Patricia Lopez-Jaramillo, Fernando Lanas, Wei Li, Yin Lu, Sun Yi, Lei Rensheng, Romaina Iqbal, Prem Mony, Rita Yusuf, Khalid Yusoff, Andrzej Szuba, Aytekin Oguz, Annika Rosengren, Ahmad Bahonar, Afzalhussein Yusufali, Aletta Elisabeth Schutte, Jephat Chifamba, Johannes F. E. Mann, Sonia S. Anand, Kuun Teo, S. Yusuf
Article
Geriatrics & Gerontology
Darryl P. Leong, Koon K. Teo, Sumathy Rangarajan, V. Raman Kutty, Fernando Lanas, Chen Hui, Xiang Quanyong, Qian Zhenzhen, Tang Jinhua, Ismail Noorhassim, Khalid F. AlHabib, Sarah J. Moss, Annika Rosengren, Ayse Arzu Akalin, Omar Rahman, Jephat Chifamba, Andres Orlandini, Rajesh Kumar, Karen Yeates, Rajeev Gupta, Afzalhussein Yusufali, Antonio Dans, Alvaro Avezum, Patricio Lopez-Jaramillo, Paul Poirier, Hosein Heidari, Katarzyna Zatonska, Romaina Iqbal, Rasha Khatib, Salim Yusuf
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2016)
Article
Multidisciplinary Sciences
Aysha Almas, Yvonne Forsell, Romaina Iqbal, Imre Janszky, Jette Moller
Article
Cardiac & Cardiovascular Systems
Rajeev Gupta, Shofiqul Islam, Prem Mony, V. Raman Kutty, Viswanathan Mohan, Rajesh Kumar, J. S. Thakur, V. Kiruba Shankar, Deepa Mohan, K. Vijayakumar, Omar Rahman, Rita Yusuf, Romaina Iqbal, Mohammed Shahid, Indu Mohan, Sumathy Rangarajan, Koon K. Teo, Salim Yusuf
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2015)
Letter
Medicine, General & Internal
Lena Jafri, Romaina Iqbal, Aysha Habib Khan
JCPSP-JOURNAL OF THE COLLEGE OF PHYSICIANS AND SURGEONS PAKISTAN
(2016)
Letter
Medicine, General & Internal
Aamir Abbas, Romaina Iqbal
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION
(2015)
Article
Medicine, General & Internal
Romaina Iqbal, Saleem Perwaiz Iqbal, Mohsin Yakub, Asa Khan Tareen, Mohammad Perwaiz Iqbal
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2015)
Article
Medicine, General & Internal
Muhammad Shahid, Romaina Iqbal, Murad M. Khan, Muhammad Zaman Khan, Uzma Shoaib Shamsi, Rooham Nakeer
JCPSP-JOURNAL OF THE COLLEGE OF PHYSICIANS AND SURGEONS PAKISTAN
(2015)
Letter
Medicine, General & Internal
Sara Habib, Romaina Iqbal, Muhammad Shahid, Aysha Habib
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION
(2015)
Letter
Medicine, General & Internal
Aysha Habib Khan, Hafsa Majid, Romaina Iqbal
JCPSP-JOURNAL OF THE COLLEGE OF PHYSICIANS AND SURGEONS PAKISTAN
(2014)
