Journal
CLINICAL BIOCHEMISTRY
Volume 42, Issue 13-14, Pages 1461-1467Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2009.06.021
Keywords
Chemiluminescence enzyme immunoassay (CLEIA); Magnetic particles (MPs); Luteinizing hormone (LH); Radioimmunoassay (RIA)
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Funding
- National Basic Research Program of China [2007CB714507]
- National Key Technology R D Program [2006AA02Z4A8]
- Research Fund for the Doctoral Program of Higher Education [20070003026]
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Objectives: A rapid and sensitive magnetic particles (MPs)-based chemiluminescence enzyme immunoassay (MPs-CLEIA) for determination of luteinizing hormone (LH) in human serum was developed and validated. Design and methods: The method was developed based on a sandwiched immunoreaction format, in which MPs served as both the solid phase and separator. The horseradish peroxidase(HRP)-luminol-H2O2 chemiluminescent system was chosen as the detection system. Results: This method had a detection limit of 0.2 mIU/mL and a linear range of 0.5-200 mIU/mL. The assay showed little cross-reactivity, and the intra-and inter-assay coefficients of variation were less than 10%. The recoveries were from 101.6% to 104.1%. The method has been successfully applied to detection of LH in human serum and showed good correlation compared with radioimmunoassay (RIA). Conclusion: The MPs-CLEIA provided apparent advantages over RIA, and facilitated the development of high-throughput screening and automated operation systems in clinical diagnosis. (C) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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