Gingival crevicular fluid PGE2, IL-1β, t-PA, PAI-2 levels in type 2 diabetes and relationship with periodontal disease

Objectives: To evaluate if type 2 diabetes mellitus increase gingival crevicular fluid (GCF) levels of prostaglandin E-2 (PGE(2)), interleukin-1beta (IL-1 beta), tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitor-2 (PAI-2). Design and methods: Seventeen type 2 diabetic patients with periodontal disease (DM), 17 other-wise healthy periodontally diseased patients (PD) and 17 systemically and periodontally healthy control subjects (H) were enrolled. Clinical periodontal measurements were recorded at six sites/tooth. GCF samples were analyzed by ELISA. Data were tested by statistical tests. Results: DM group revealed lower IL-1 beta levels than PD group (p<0.01). PGE(2), t-PA and PAI-2 levels were similar in DM and PD groups (p>0.05). PGE(2), t-PA levels were higher in DM and PD groups than H group (p<0.05). PAI-2 level was higher in DM group than H group (p<0.05). GCF total amount of PGE(2) in DM group exhibited significant correlations with all clinical periodontal measurements (p<0.05). Conclusion: Type 2 diabetes in this study seems not to increase GCF levels of the evaluated inflammatory mediators. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.