Journal
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Volume 152, Issue 1, Pages 72-80Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-2249.2008.03610.x
Keywords
activation markers; HIV-1; interleukin-7; interleukin-7 receptor; T cell subsets
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Interleukin (IL)-7 and its receptor (IL-7R alpha) play important roles in regulating lymphopoiesis. Previous studies have reported that human immunodeficiency virus-1 (HIV-1) viraemia affects the expression of IL-7R alpha, but its effects on CD4(+) and CD8(+) T cell memory subsets have not been studied. Using eight-colour flow cytometry, we compared the immunophenotypic patterns of CD4(+) and CD8(+) T cell subsets expressing IL-7R alpha and activation markers, as well as circulating IL-7 levels, in three well-defined groups of HIV-1-infected subjects: successfully treated, viraemic and long-term non-progressor (LTNP). Compared with successfully treated and LTNP subjects, viraemic patients had reduced expression of IL-7R alpha on both CD4(+) and CD8(+) T cells, particularly on central and effector memory T cell compartments, and substantially elevated expression of activation markers on CD8(+) T cell subsets. Circulating IL-7 levels were correlated negatively with the number of CD4(+) and CD8(+) T cell subsets expressing IL-7R alpha; these associations were stronger with CD4(+) T cell subsets and mainly with central and effector memory cells. The expression of activation markers on CD4(+) and CD8(+) cell T subsets was not related to circulating IL-7 levels. A strong negative correlation was observed between central memory CD4(+) or CD8(+) T cells expressing IL-7R alpha and those expressing activation markers, independently of IL-7 levels. Collectively, these results provide further insight on the role of unsuppressed viral load in disrupting the IL-7/IL-7R alpha system and contributing to HIV-1 disease progression.
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