Article
Microbiology
Laurine Kaul, Adrian I. Abdo, Tom Coenye, Bastiaan P. Krom, Michel A. Hoogenkamp, Andrew C. W. Zannettino, Regine Suess, Katharina Richter
Summary: This study describes the antibacterial properties of a combination treatment of diethyldithiocarbamate (DDC) and copper ions (Cu2+), which showed synergistic and additive effects against planktonic and biofilm cultures of Staphylococcus aureus and Staphylococcus epidermidis, with low cytotoxicity.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Denis Swolana, Robert D. Wojtyczka
Summary: The article introduces the effects of Staphylococcus epidermidis and Staphylococcus aureus on human health, as well as silver nanoparticles as a potential new substance for neutralizing bacterial cells. By reviewing the data on the activity of silver nanoparticles in inhibiting bacterial growth and biofilm formation, it provides insights for future research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Annalisa Chianese, Carla Zannella, Francesco Foglia, Bianca Maria Nastri, Alessandra Monti, Nunzianna Doti, Gianluigi Franci, Anna De Filippis, Massimiliano Galdiero
Summary: The resistance of pathogenic microorganisms to antimicrobial agents has become a severe public health problem. The World Health Organization is searching for new drugs to combat these emerging pathogens. In this study, the antibacterial activity of the AMP Hylin-a1 against Staphylococcus aureus strains was evaluated. Hylin-a1 showed significant bacteriostatic activity and was effective against methicillin-resistant S. aureus. It also regulated the inflammatory response and showed potential for treating various clinical manifestations caused by S. aureus.
Article
Veterinary Sciences
Laura Miguel-Pastor, Katy Satue, Deborah Chicharro, Pau Pelaez, Marta Torres-Torrillas, Jose M. Carrillo, Jose J. Ceron, Joaquin J. Sopena, Monica Rubio
Summary: Feline leukemia virus (FeLV) is a chronic disease that weakens a cat's immune system. Platelet-rich plasma (PRP) and growth factors (GFs) determination offer potential alternative treatment for improving quality of life in FeLV cats. This study aimed to determine the concentration of platelets, red blood cells, white blood cells, and growth factors in whole blood, PRP, and platelet-poor plasma fractions using a standardized protocol.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Suzana Helena Campelo Nogueira-Lima, Paulo Wender P. Gomes, Kely. C. C. Navegantes-Lima, Jose Diogo E. Reis, Alice Rhelly Veloso Carvalho, Sonia das Gracas Santa R. Pamplona, Abraao de Jesus B. Muribeca, Milton. N. N. Da Silva, Marta. C. C. Monteiro, Consuelo Yumiko Yoshioka e Silva
Summary: Deguelia nitidula roots have significant bactericidal activity against Staphylococcus aureus, especially the SREFr3 fraction which shows safe and effective antibacterial activity and has a high selectivity index, making it a potential candidate for further bio-guided research and natural drug development.
Article
Agriculture, Multidisciplinary
Hang Sun, Mohammad Fawad Ansari, Bo Fang, Cheng-He Zhou
Summary: Berberine-hybridized benzimidazoles, such as the 2,4-dichlorobenzyl derivative 7d, showed potent antibacterial efficacy against Staphylococcus aureus, with the ability to eradicate bacterial biofilm and low toxicity towards mammalian cells. Combination with norfloxacin enhanced the antibacterial efficacy, while development of bacterial resistance was not observed. Mechanism studies indicated that the active molecule 7d damaged membrane integrity, stimulated ROS generation, and bound with DNA and S. aureus sortase A, exerting powerful antibacterial ability in treating S. aureus in the livestock industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Infectious Diseases
Libardo Suarez, Andres Pereira, William Hidalgo, Nelson Uribe
Summary: The semi-purified fractions from the mucus secretion of Achatina fulica showed inhibition of growth and biofilm formation of Staphylococcus aureus, as well as affecting the activity of virulence factors produced by the bacteria.
Article
Biochemistry & Molecular Biology
Chunling Wei, Peiwu Cui, Xiangqian Liu
Summary: Antibacterial resistance is a serious global health problem, and scientists are focusing on developing new antibacterial drugs from natural products. This study investigated the antibacterial activity and mechanism of madecassic acid (MA), a compound isolated from Chinese medicine. It was found that MA had inhibitory effects on eight pathogenic bacteria, with a minimum inhibitory concentration of 31.25μg/mL for Staphylococcus aureus.
Article
Infectious Diseases
Jin-Mi Park, Dae-Sung Ko, Hee-Soo Kim, Nam-Hyung Kim, Eun-Kyoung Kim, Young-Hye Roh, Danil Kim, Jae-Hong Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Summary: Researchers have developed chimeric lysins containing various combinations of cell wall-lysing and cell-wall-binding domains for antibacterial purposes. They improved a cell-free expression system for screening the activity of these chimeric lysin candidates. They screened and compared the antibacterial activity of the candidates and found that the CHAP domain of ALS2 showed relatively strong activity and may be useful in controlling bovine mastitis.
Article
Polymer Science
Joanna Jaworska, Katarzyna Jelonek, Tomasz J. Wasik, Maria Miklasinska-Majdanik, Malgorzata Kepa, Jolanta Bratosiewicz-Wasik, Bozena Kaczmarczyk, Andrzej Marcinkowski, Henryk Janeczek, Janusz Szewczenko, Wojciech Kajzer, Monika Musial-Kulik, Janusz Kasperczyk
Summary: Biodegradable P(L/TMC) coatings loaded with single or multiple antibiotics were developed to reduce infections associated with metallic implants. The drug delivery systems showed stable degradation process, changes in thermal properties, and antibacterial activity against different strains. However, combining ciprofloxacin, fusidic acid, and azithromycin into one coating did not enhance the antibacterial effect.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Food Science & Technology
Yupan Zhu, Hangbo Xu, Dongjie Cui, Renwu Zhou, Yanping Wang, Aswathi Soni, Gale Brightwell, Jie Zhuang, Ruonan Ma, Zhen Jiao
Summary: This study investigated the effects of atmospheric cold plasma (ACP) treatment on the antioxidant pigment STX in S. aureus. Results showed that ACP treatment significantly decreased the survival rate of S. aureus and caused a decrease in STX content, making the bacteria more susceptible to hydrogen peroxide attack. ACP treatment also resulted in a decrease in cell membrane potential, accumulation of reactive oxygen species, and cell necrosis.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2023)
Article
Immunology
Quang Vinh Ngo, Larissa Faass, Aline Saehr, Dagmar Hildebrand, Tatjana Eigenbrod, Klaus Heeg, Dennis Nurjadi
Summary: Staphylococcus aureus can cause infections, but it can also colonize the skin without inducing inflammatory response in some individuals. On the other hand, Staphylococcus epidermidis can permanently colonize the skin without activating skin inflammation. Our study suggests that the internalization of S. aureus and the subsequent sensing of bacterial nucleic acid are essential for initiating inflammatory response in skin cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Thibaut Barbier, Alexia Barbry, Jeremy Magand, Cedric Badiou, Floriane Davy, Anne Baudouin, Yves Queneau, Oana Dumitrescu, Gerard Lina, Laurent Soulere
Summary: In this study, new series of compounds were synthesized by combining the benzo[b]thiophene nucleus and the acylhydrazone functional group for screening against Staphylococcus aureus. Among the compounds tested, (E)-6-chloro-N'-(pyridin-2-ylmethylene)benzo[b]thiophene-2-carbohydrazide (II.b) showed promising antimicrobial activity with a minimal inhibitory concentration of 4 μg/mL against S. aureus strains, including clinically isolated strains resistant to methicillin and daptomycin.
Article
Pharmacology & Pharmacy
Getha Krishnasamy, Muhammad-Syamil Azahar, Shariffah-Nurhidayah S. A. Rahman, Vimalah Vallavan, Noraziah M. Zin, Mazlyzam A. Latif, Masahiro Hatsu
Summary: Aurisin A, a dimeric sesquiterpenoid isolated from wild bioluminescent basidiomycetes, showed strong anti-MRSA activity, with a higher potency than fusidic acid. It also displayed synergy with other antibiotics against MRSA.
SAUDI PHARMACEUTICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Mathilde Fourniere, Gilles Bedoux, Djouhar Souak, Nathalie Bourgougnon, Marc G. J. Feuilloley, Thomas Latire
Summary: The study demonstrates that poly- and oligosaccharide fractions of Ulva sp. have notable biological activities on cutaneous bacteria, especially towards acneic and non-acneic strains of Cutibacterium acnes, supporting their potential use in dermo-cosmetic applications.
Article
Ophthalmology
Eduardo Anitua, Maria de la Fuente, Ronald M. Sanchez-Avila, Borja de la Sen-corcuera, Jesus Merayo-Lloves, Francisco Muruzabal
Summary: Recent research has shown that plasma rich in growth factors (PRGF) eye drops have higher biological activity and can reduce the expression of fibrosis markers compared to insulin and autologous serum eye drops, making them a more effective therapy for the treatment of ocular surface diseases.
CURRENT EYE RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Eduardo Anitua, Carlos Flores, Joaquin Duran-Cantolla, Gabriela Zamora Almeida, Mohammad Hamdan Alkhraisat
Summary: This study aimed to assess the frequency of obstructive sleep apnea (OSA) in patients presenting with tooth fracture. The study found that among patients with tooth fractures, 21 had OSA compared to 12 in the control group. Age was the only factor significantly associated with tooth fracture. There was a significant relationship between OSA and tooth fracture in patients aged 40 and above.
INTERNATIONAL JOURNAL OF PERIODONTICS & RESTORATIVE DENTISTRY
(2023)
Editorial Material
Engineering, Environmental
Unax Lertxundi, Saioa Domingo-Echaburu, Susana Barros, Miguel Machado Santos, Teresa Neuparth, Jose Benito Quintana, Rosario Rodil, Rosa Montes, Gorka Orive
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Eduardo Anitua, Carlos Flores, Sofia Fernandez-de-Retana, Beatriz Anitua, Mohammad H. Alkhraisat
Summary: Short dental implants are a viable alternative to longer implants and bone augmentation procedures. Immediate loading of these short implants has been shown to be predictable, with no significant differences in survival and marginal bone loss compared to delayed loading. This retrospective study reported the mid-term outcomes (5-year) of short implants and suggests the need for future prospective studies to confirm these results.
INTERNATIONAL JOURNAL OF PERIODONTICS & RESTORATIVE DENTISTRY
(2023)
Article
Engineering, Biomedical
Eduardo Anitua, Mar Zalduendo, Maria Troya, Roberto Tierno, Mohammad H. Alkhraisat
Summary: Scaffolds play a crucial role in tissue regeneration by providing structural support and interacting with cells and bioactive molecules. Platelet Rich Plasma (PRP) fibrin, as a successful scaffold, has significant biological effects and clinical potential. This study investigated the influence of cellular composition on fibrin membrane stability and remodeling cell activity, showing that leukocytes in PRP fibrin membranes reduce scaffold stability and induce behavioral changes in fibroblasts.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Dermatology
Alejandro Garcia, M. R. Navarro, Ana Ramirez, Ander Pino, Ariadna Navarro, Isabel Moles, Estefania Gallego, Eduardo Anitua
Summary: This study retrospectively analyzed the adjuvant use of plasma rich in growth factors (PRGF) in the management of frontal fibrosing alopecia (FFA). The results showed that PRGF treatment significantly reduced hair loss, induced hair regrowth, and alleviated inflammation compared to conventional therapy.
JOURNAL OF CUTANEOUS MEDICINE AND SURGERY
(2023)
Article
Medicine, General & Internal
Pedro P. Rodriguez-Calvo, Ignacio Rodriguez-Una, Andres Fernandez-Vega-Cueto, Ronald M. Sanchez-Avila, Eduardo Anitua, Jesus Merayo-Lloves
Summary: This study evaluated the utility and safety of plasma rich in growth factors immunosafe eye drops (is-ePRGF) in the postoperative treatment of nonpenetrating deep sclerectomy (NPDS). The results showed that the use of is-ePRGF can reduce intraocular pressure and the rate of complications, making it a possible safe adjuvant for surgical success.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Alberto Barros, Javier Lozano-Sanroma, Juan Queiruga-Pineiro, Luis Fernandez-Vega Cueto, Eduardo Anitua, Ignacio Alcalde, Jesus Merayo-Lloves
Summary: This study used in vivo corneal confocal microscopy to analyze the changes in corneal innervation in patients with Evaporative and Aqueous Deficient Dry Eye and treated with a standard treatment for Dry Eye Disease in combination with Plasma Rich in Growth Factors. The results showed that the combined treatment therapy with PRGF outperformed the standard treatment therapy in terms of subbasal nerve plexus regeneration and tear film stability, with the most significant improvement observed in the ADDE subtype.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Silvia Hernaez Crespo, Jose Israel Lopez Mirones, Andres Canut, Rodrigo Alonso, Ilargi Martinez-Ballesteros, Irati Martinez-Malaxetxebarria
Summary: The study characterized the virulence and antimicrobial resistance of different strains of Arcobacter butzleri through genomic analysis and in vitro experiments. It identified genetic differences between strains and discovered genes associated with antibiotic resistance and pathogenicity. These findings contribute to the understanding of the mechanisms behind Arcobacter butzleri-induced diseases.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eduardo Anitua, Francisco Muruzabal, Maria de la Fuente, Susana Del Olmo-Aguado, Mohammad H. H. Alkhraisat, Jesus Merayo-Lloves
Summary: This study evaluates the effectiveness of a novel plasma rich in growth factors (PRGF) membrane in reducing oxidative stress in retinal pigment epithelial cells (ARPE-19 cells) exposed to blue light. Different formulations derived from PRGF were tested on ARPE-19 cells, and it was found that they increased mitochondrial viability, cell survival, and decreased reactive oxygen species (ROS) production and heme oxygenase -1 (HO-1) expression. The PRGF membrane also increased ZO-1 expression in ARPE-19 cells exposed to blue light.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Eduardo Anitua, Maria Troya, Juan Manuel Falcon-Perez, Silvia Lopez-Sarrio, Esperanza Gonzalez, Mohammad H. Alkhraisat
Summary: The use of platelet-rich plasma (PRP) has attracted increasing interest due to its rich content of growth factors, cytokines, chemokines, and other biomolecules. Recent research has focused on platelets' released extracellular vesicles (EVs) and their role in intercellular communication. This review aims to compile the current evidence on PRP-derived EVs and evaluate their advantages and limitations for clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Eduardo Anitua, Roberto Prado, Jorge Guadilla, Mohammad H. Alkhraisat, Patricia Laiz, Sabino Padilla, Montserrat Garcia-Balletbo, Ramon Cugat
Summary: The treatment of chondral and osteochondral defects is challenging and tissue engineering offers a potential solution. In this study, the use of an autologous cartilage construct embedded in plasma rich in growth factors (PRGF) was investigated. The research found that the cartilage fragments contained viable chondrocytes that could proliferate and synthesise extracellular matrix, suggesting a potential mechanism behind the clinical success of this technique.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Environmental Sciences
Iker Egana, Saioa Domingo-Echaburu, Gorka Orive, Unax Lertxundi
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Medicine, General & Internal
Antonio Rios Luna, Homid Fahandezh-Saddi Diaz, Manuel Villanueva Martinez, Roberto Iglesias, Roberto Prado, Sabino Padilla, Eduardo Anitua
Summary: The aim of this study was to investigate the use of office-based ultrasound-guided intraosseous and intra-articular infiltrations of plasma rich in growth factors (PRGF) for patients with moderate and severe knee osteoarthritis (KOA). The study included 79 patients with grade 3-4 KOA, who were treated with a minimally invasive technique using local anesthesia. The results showed significant improvement in knee injury and osteoarthritis outcome score (KOOS) after the treatment.
JOURNAL OF CLINICAL MEDICINE
(2023)