Article
Endocrinology & Metabolism
Qicheng Ni, Jiajun Sun, Yichen Wang, Yanqiu Wang, Jingwen Liu, Guang Ning, Weiqing Wang, Qidi Wang
Summary: This study reveals that DNMT3A-dependent DNA methylation and PRC2-dependent H3K27me3 modification are major epigenetic silencing mechanisms responsible for the functional immaturity of Raptor-deficient β-cells. Overexpression of DNMT3A partially reverses the immature transcriptome pattern and restores impaired GSIS in Raptor-deficient β-cells. Additionally, Raptor directly regulates PRC2/EED and H3K27me3 expression levels, as well as a group of immature genes marked with H3K27me3.
MOLECULAR METABOLISM
(2022)
Review
Biochemistry & Molecular Biology
Thamila Kerkour, Catherine Zhou, Loes Hollestein, Antien Mooyaart
Summary: Studying primary melanoma and its metastasis is beneficial for understanding tumor biology and determining drug targets. This study analyzed literature systematically, evaluated gene concordance, and assessed heterogeneity. The results showed high concordance of drug targets during melanoma progression, but significant heterogeneity in gene consistency, requiring further validation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dermatology
Ida M. Heerfordt, Peter A. Philipsen, Jeppe D. Andersen, Linnea Langhans, Grethe Schmidt, Niels Morling, Hans Christian Wulf
Summary: Using tape strip derived RNA, this study developed a technique to differentiate cutaneous malignant melanoma (CMM) from nevi with 100% sensitivity. The technique reduced the unnecessary surgical removal of benign lesions by one-third without overlooking any CMMs.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Oncology
Jiaoquan Chen, Nanji Yu, Shanshan Ou, Xue Wang, Huaping Li, Huilan Zhu
Summary: This study comprehensively investigated the association between SMARCD1 expression and various factors in skin cutaneous melanoma (SKCM), including prognosis, tumor microenvironment (TME), immune infiltration, tumor mutational burden (TMB), and microsatellite instability (MSI). The aberrant expression of SMARCD1 was found to be strongly correlated with overall survival (OS) and progression-free survival (PFS) across multiple cancers. SMARCD1 is likely involved in various signaling pathways and biological processes in SKCM.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Medical Laboratory Technology
Woo Cheal Cho, Wei-Lien Wang, Denai R. Milton, Davis R. Ingram, Priyadharsini Nagarajan, Jonathan L. Curry, Doina Ivan, Alexander J. Lazar, Wen-Jen Hwu, Victor G. Prieto, Carlos A. Torres-Cabala, Phyu P. Aung
Summary: The study evaluated the frequency and pattern of TERT immunoreactivity in acral lentiginous melanomas (ALMs) and found that TERT expression was more frequent and intense in ALMs compared to other melanocytic lesions. However, there was no significant correlation between TERT expression and patient survival in this study, suggesting the need for further research to elucidate the prognostic value of TERT expression in ALMs.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Jingwen Liu, Meiyan Sun, Kwang Bog Cho, Xiang Gao, Bin Guo
Summary: The research team successfully designed a new strategy by combining CRISPR-Cas9 technology with HDAC1 epigenetic silencing to silence KRAS and inhibit the growth and proliferation of cancer cells. This strategy holds promise as a potential effective approach for treating cancers driven by KRAS mutations in the future.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ka-Wing Fong, Jonathan C. Zhao, Xiaodong Lu, Jung Kim, Andrea Piunti, Ali Shilatifard, Jindan Yu
Summary: PALI1 is a newly identified accessory protein that plays a role in advanced prostate cancer. It competes with JARID2 for binding to the PRC2 core subunit SUZ12 and interacts with the H3K9 methyltransferase G9A, forming a unique super-complex involved in dual H3K9/K27 methylation and gene repression. PALI1 and G9A promote prostate cancer cell proliferation and invasion, and drive tumor growth in xenograft models.
Article
Cell Biology
Yuka Tanaka, Takamichi Ito, Yumiko Kaku-Ito, Keiko Tanegashima, Gaku Tsuji, Makiko Kido-Nakahara, Yoshinao Oda, Takeshi Nakahara
Summary: This study evaluated the potential of HER3-targeted therapy for acral melanoma (AM) by investigating the expression and function of HER3. The results showed that HER3 was highly expressed in AM tissues and significantly correlated with patient's disease-free survival. Inhibition of HER3 reduced the anchorage-independent growth of AM cells likely through affecting the nuclear translocation of Yes-associated protein.
CELL DEATH DISCOVERY
(2023)
Article
Biology
Leonardo Vinicius Monteiro de Assis, Jose Thalles Lacerda, Maria Nathalia Moraes, Omar Alberto Dominguez-Amorocho, Gabriela Sarti Kinker, Davi Mendes, Matheus Molina Silva, Carlos Frederico Martins Menck, Niels Olsen Saraiva Camara, Ana Maria de Lauro Castrucci
Summary: Our study shows that the absence of Opsin 4 (Opn4) can slow down cell proliferation and tumor progression in cutaneous melanoma. Furthermore, Opn4(KO) tumors exhibit a higher immune system response. The evaluation of TCGA database confirms these findings in human melanoma.
COMMUNICATIONS BIOLOGY
(2022)
Article
Immunology
Victoria Anne Brentville, Peter Symonds, JiaXin Chua, Anne Skinner, Ian Daniels, Katherine Wendy Cook, Sasa Koncarevic, Roxana Martinez-Pinna, Sabaria Shah, Ruhul Hasan Choudhury, Poonam Vaghela, Daisy Weston, Abdullah Al-Omari, James Davis, Lindy G. Durrant
Summary: Post translational modification of proteins, such as citrullination, plays a significant role in immune recognition. Targeting citrullinated GRP78 shows potential for cancer therapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Tianhao Li, Liquan Wang, Nanze Yu, Ang Zeng, Jiuzuo Huang, Xiao Long
Summary: This study found that CCP-related genes are associated with TNM stage, age, pathological grade, and Breslow depth in cutaneous melanoma patients. These genes play a crucial role in disease prognosis. Further analysis revealed their involvement in DNA replication, G1-S specific transcription factor, mitotic spindle checkpoint, and cell cycle. Moreover, there is a negative association between CCP-related genes and the abundance of innate immune cells. Knockdown of CDCA3, a CCP-related gene, significantly suppressed the proliferation and migration ability of cutaneous melanoma cells.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
ShengYong Long, Xuan Fen Zhang
Summary: AURKA is a core hub gene driving the occurrence and development of SKCM. It regulates the infiltration level of various immune cells in the tumor microenvironment, reshapes the immunosuppressive tumor microenvironment, and induces apoptosis and hypoxia. It is a prognostic biomarker and potential therapeutic target in SKCM. The small-molecule compound ZNC97018978 targeting AURKA can inhibit the proliferation, invasion, and metastasis of SKCM by arresting the cell cycle and inducing apoptosis. Further studies should be conducted to determine its effectiveness and safety as a potential treatment for cutaneous melanoma.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Oncology
Orsolya Pipek, Laura Vizkeleti, Viktoria Doma, Donat Alpar, Csaba Bödör, Sarolta Karpati, Jozsef Timar
Summary: Malignant melanoma of the skin, primarily on UV-exposed skin, is commonly associated with mutant BRAF, RAS, and KIT oncogenes. The genetic makeup of triple-wild-type melanoma (BRAF, NRAS, and NF1) has been studied before, but with mixed rare and common histopathological subtypes. In this study, whole genome sequencing was used to characterize triple-wild-type melanoma, excluding rare histopathological subtypes. Unrecognized drivers were identified among passenger mutations in some cases.
Article
Biochemistry & Molecular Biology
Abdulraheem Alshareef, Anthea C. Peters, Pascal Gelebart, Will Chen, Raymond Lai
Summary: The constitutive activation of the Wnt canonical pathway (WCP) in mantle cell lymphoma (MCL) is mainly due to gene methylation/silencing of WIF1, which promotes cell growth. Gene transfection of WIF1 into cells significantly reduces cell growth and results in downregulation of various proteins in WCP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dermatology
Sara Peru, Martina Prochazkova-Carlotti, Floriane Cherrier, Joanne Velazquez, Elodie Richard, Yamina Idrissi, David Cappellen, Lamia Azzi-Martin, Anne Pham-Ledard, Marie Beylot-Barry, Jean-Philippe Merlio, Sandrine Poglio
Summary: Cutaneous T-cell lymphoma (CTCL) is characterized by abnormal infiltration of T lymphocytes in the skin. In this study, the researchers found that the expression of cutaneous lymphocyte antigen (CLA) in CTCL cells is crucial for their migration and survival. The use of a CLA antibody significantly reduced the migration and survival of CTCL cells both in vitro and in vivo. These findings suggest that inhibiting CLA could be a potential therapeutic strategy for CTCL patients.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Pathology
Louise Johnston, Michael Power, Philip Sloan, Anna Long, Angela Silmon, Ben Chaffey, Andrea Jane Lisgo, Liam Little, Ellen Vercauteren, Torben Steiniche, Tine Meyer, John Simpson
JOURNAL OF CLINICAL PATHOLOGY
(2018)
Article
Oncology
Cecilie Abildgaard, Christina Dahl, Ahmad Abdul-Al, Annette Christensen, Per Guldberg
Article
Cell Biology
Line B. Nielsen, Nina Dabrosin, Karen Sloth, Marie L. Bonnelykke-Behrndtz, Torben Steiniche, Johanne Lade-Keller
Article
Oncology
Johanne Lade-Keller, Sakineh Yuusufi, Rikke Riber-Hansen, Torben Steiniche, Magnus Stougaard
Article
Oncology
Isabella Maria Dias Payao Ortiz, Mateus Camargo Barros-Filho, Mariana Bisarro dos Reis, Caroline Moraes Beltrami, Fabio Albuquerque Marchi, Hellen Kuasne, Luisa Matos do Canto, Julia Bette Homem de Mello, Cecilie Abildgaard, Clovis Antonio Lopes Pinto, Luiz Paulo Kowalski, Silvia Regina Rogatto
CLINICAL EPIGENETICS
(2018)
Review
Oncology
Cecilie Abildgaard, Luisa M. Do Canto, Karina D. Steffensen, Silvia R. Rogatto
FRONTIERS IN ONCOLOGY
(2020)
Review
Oncology
Naiade Calanca, Cecilie Abildgaard, Claudia Aparecida Rainho, Silvia Regina Rogatto
Article
Pathology
Ea Tonnesen, Johanne Lade-Keller, Magnus Stougaard
Summary: The study found that implementing NGS in routine molecular diagnostics for lung adenocarcinomas provides significant advantages over qPCR, allowing for the identification of more uncommon but clinically important gene mutations.
Article
Pathology
Demet Ozcan, Johanne Lade-Keller, Trine Tramm
Summary: The study found that a substantial proportion of PD-L1 negative TNBC patients have complete or partial loss of MMR and/or high TILs, suggesting that supplementing PD-L1 examination with these biomarkers may help identify TNBC patients suitable for immunotherapy.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Pathology
Anita Tranberg Simonsen, Amalie Utke, Johanne Lade-Keller, Lasse Westphal Thomsen, Torben Steiniche, Magnus Stougaard
Summary: This study presents a small targeted expression panel with the potential to perform molecularly based histological classification and fusion gene identification in non-small-cell lung cancer (NSCLC) patients. The panel showed agreement with the original immunohistochemistry-based classification and successfully detected fusion transcripts in patients with fusion genes. Additionally, the molecular assessment of PD-L1 was consistent with the original assessment by immunohistochemistry.
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2022)
Article
Oncology
Lisa Lokso Dietz, Natasja Toft Furman, Trine Vilsboll Larsen, Tina Fuglsang Daugaard, Emil Aagaard Thomsen, Johanne Lade Keller, Lars Aagaard, Boe Sandahl Sorensen, Anders Lade Nielsen
Summary: Researchers have discovered eight different PD-L2 isoforms in non-small cell lung cancer cells, generated from the PDCD1LG2 gene through alternative splicing. The most prominent alternative splicing event is the extension of exon 6, resulting in the PD-L2 isoform V with a cytoplasmic domain extension. These observations of different cytoplasmic domains are important for further understanding of the PD-1 immune checkpoint pathway.
JOURNAL OF IMMUNOTHERAPY
(2022)
Article
Pathology
Carina Mellemgaard, Ib Jarle Christensen, Giedrius Salkus, Pia Wirenfeldt Staun, Niels Korsgaard, Kim Hein Lindahl, Mathilde Skaarup Larsen, Siri Klausen, Johanne Lade-Keller
Summary: The aim of the study was to optimize the melanoma sentinel node (SN) pathology protocol by reducing the workload without compromising the metastasis detection rate. Through implementing a protocol that examines 3 levels 300μm apart combined with immunohistochemistry (IHC), it was found that the metastasis detection rate was similar to the protocol that examines 6 levels 150μm apart. Therefore, it is recommended that the future melanoma SN guidelines consider using the three-level method to reduce overall workload.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Pathology
Ea Maria Tonning Tonnesen, Magnus Stougaard, Peter Meldgaard, Johanne Lade-Keller
Summary: This study investigates the association between oncogenic alterations and PD-L1 expression in lung adenocarcinomas, as well as the prognostic value of KRAS and/or TP53 mutations in patients treated with immunotherapy. The results suggest that KRAS mutations and higher expression of PD-L1 are associated. Additionally, concomitant TP53 and KRAS mutations may serve as a potential biomarker for improved survival with immunotherapy.
JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Oncology
Trine V. Larsen, Nina Dybdal, Tina F. Daugaard, Johanne Lade-Keller, Lin Lin, Boe S. Sorensen, Anders L. Nielsen
Summary: This study investigates the role of PD-L1 DNA methylation in regulating and predicting PD-L1 expression in non-small-cell lung cancer (NSCLC) patients undergoing immunotherapy. The analysis of tumor biopsies and cell lines reveals that PD-L1 DNA methylation status influences its expression. However, the correlation between methylation and expression is weak in NSCLC tumor samples. This study emphasizes the importance of further research to improve the effectiveness of PD-1/PD-L1 immunotherapy in NSCLC.
Article
Biochemistry & Molecular Biology
Emmeli Mikkelsen, Berthold Huppertz, Ripudaman Singh, Katarina Ravn, Lotte Hatt, Mogens Kruhoffer, Rheanna Urrabaz-Garza, Niels Uldbjerg, Ramkumar Menon, Torben Steiniche
Summary: Unique markers in fetal membrane cells may be key in the search for biomarkers for preterm prelabor rupture of the fetal membranes (pPROM) in maternal blood. Through transcriptomic analysis and immunohistochemistry, markers exclusively expressed in fetal membranes were identified and their localization confirmed. These findings contribute to the understanding of the biological significance of these markers in the maternal circulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)