Article
Medicine, General & Internal
Kamala Vanarsa, Jessica Castillo, Long Wang, Kyung Hyun Lee, Claudia Pedroza, Yair Lotan, Chandra Mohan
Summary: This study identified potential biomarkers for bladder cancer diagnosis and disease progression using aptamer screening and systems biology analysis. Urine d-dimer showed high sensitivity (97%) for bladder cancer diagnosis, while urine IL-8 and IgA were associated with disease progression. These biomarkers could be used for initial triage and patient follow-up, reducing the need for invasive cystoscopy-based diagnostic methods.
Article
Oncology
Anders Vikerfors, Sabina Davidsson, Janusz Frey, Tomas Jerlstrom, Jessica Carlsson
Summary: The study found that high serum PD-L1 levels are associated with aggressive disease and development of metastatic disease in BC patients, but no significant difference was found in urine sPD-L1 levels in BC patients.
Article
Biochemical Research Methods
Masataka Nakagawa, Yui Tomioka, Chiaki Sakuma, Yasunori Kurosawa, Takashi Shibata, Tsutomu Arakawa, Teruo Akuta
Summary: A new protocol for conducting two-dimensional (2D) electrophoresis was developed by combining agarose native gel electrophoresis with sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) or flat SDS agarose gel electrophoresis. The technique utilizes His/MES buffer during the first-dimensional agarose native gel electrophoresis to visualize basic and acidic proteins in their native states or complex structures. The gel strip from the first-dimensional agarose gel electrophoresis is soaked in SDS and placed on top of vertical SDS-PAGE gels or flat SDS-MetaPhor high-resolution agarose gels for the second-dimensional separation.
Article
Chemistry, Analytical
Shingo Saito, Junko Ishikawa, Moe Ono, Yuiko Tasaki-Handa, Masami Shibukawa
Summary: This paper presents a novel method, called HAC-2D urea PAGE, for analyzing Fe3+-bound transferrin species in biological samples. The method dissociates Fe3+ ions bound to the protein and determines their concentration using PAGE with a fluorescent probe. The distribution of Fe-bound transferrin species in a standard human serum sample was successfully determined, and the concentration of free Fe3+ in the serum was estimated.
ANALYTICAL SCIENCES
(2023)
Article
Plant Sciences
Zheng-Jun Guan, Min Zheng, Zhi-Xi Tang, Wei Wei, C. Neal Stewart Jr
Summary: In this study, differential expression of total protein in leaves of two transgenic oilseed rape lines and their non-transgenic parent plant was analyzed. Fourteen differential protein spots were identified, including eleven upregulated and three downregulated proteins, involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. These changes in protein spots may be attributed to the insertion of foreign transgenes in transgenic oilseed rape, but transgenic manipulation does not necessarily cause significant changes in the proteomes of oilseed rape.
Article
Biochemistry & Molecular Biology
Emmanouil Mavrogeorgis, Harald Mischak, Agnieszka Latosinska, Justyna Siwy, Vera Jankowski, Joachim Jankowski
Summary: In recent years, CE-MS has been increasingly used in clinical research for chronic and age-associated diseases, allowing for comprehensive assessment of small molecular weight proteins and peptides. The study evaluated the analytical performance of CE-MS in terms of reproducibility, variability, and efficiency in peptide detection, showing high correlation among different runs and low variation in signal intensity. These findings support the value of CE-MS for clinical application.
Article
Polymer Science
Hiroyuki Ohshima
Summary: This study develops a general theory for the time-dependent transient electrophoresis of a weakly charged spherical colloidal particle in a polymer gel medium. The Laplace transform of the transient electrophoretic mobility is derived by considering the hydrodynamic interaction between the particle and the gel medium based on the Brinkman-Debye-Bueche model. The theory covers both transient gel electrophoresis and transient free-solution electrophoresis, with the relaxation time for the former being shorter and decreasing as the Brinkman screening length decreases.
Article
Oncology
Eisuke Tomiyama, Kyosuke Matsuzaki, Kazutoshi Fujita, Takashi Shiromizu, Ryohei Narumi, Kentaro Jingushi, Yoko Koh, Makoto Matsushita, Kosuke Nakano, Yujiro Hayashi, Cong Wang, Yu Ishizuya, Taigo Kato, Koji Hatano, Atsunari Kawashima, Takeshi Ujike, Motohide Uemura, Tetsuya Takao, Jun Adachi, Takeshi Tomonaga, Norio Nonomura
Summary: Proteomic analysis of urinary EVs combined with Te-EVs allows for selective detection of urinary BCa biomarkers.
Review
Cell Biology
Seung-Hwan Jeong, Ja Hyeon Ku
Summary: This paper reviewed urinary markers for bladder cancer and compared their efficacies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biology
Nassib Abou Heidar, Tariq A. Bhat, Usma Shabir, Ahmed A. Hussein
Summary: Bladder cancer is a common cancer worldwide, and most patients have non-muscle invasive disease. Transurethral resection of bladder tumor (TURBT) with adjuvant intravesical therapy is the standard treatment. Urine, previously thought to be sterile, has been found to have a urinary microbiome associated with urological diseases. The urinary microbiome may serve as a biomarker for risk stratification and response prediction in bladder cancer.
Article
Biology
Jorge Cebrian, Victor Martinez, Pablo Hernandez, Dora B. Krimer, Maria-Jose Fernandez-Nestosa, Jorge B. Schvartzman
Summary: The study utilized two-dimensional agarose gel electrophoresis to investigate the efficiency of three type II DNA topoisomerases on partially replicated bacterial plasmids. The results revealed the preferential functions and some redundancy, shedding light on their abilities to resolve supercoiling and pre-catenanes in vitro.
Article
Oncology
Maria Eldh, Michael Mints, Stefanie Hiltbrunner, Sam Ladjevardi, Farhood Alamdari, Markus Johansson, Tomasz Jakubczyk, Rosanne E. Veerman, Ola Winqvist, Amir Sherif, Susanne Gabrielsson
Summary: The study investigated the release of exosomes from urinary bladder tissue post-TUR-B in UBC patients to find cancer-promoting exosomes originating from both tumour-proximal and tumour-distant sites, highlighting the need for early radical cystectomy. This suggests that the entire bladder tissue may release exosomes contributing to metastasis despite the absence of detectable tumour, supporting the role of exosomes as potential biomarkers and mediators of metastatic spread. Additionally, the enrichment of cancer-related pathways in tissue-derived exosomes from both sites strengthens the hypothesis and emphasizes the necessity for early removal of the bladder in UBC patients.
Article
Biochemistry & Molecular Biology
Agata Piasecka, Andrea Baier
Summary: This study investigated the impact of three trophic modes on the growth parameters and biochemical composition of Chlorella species. The results showed that mixotrophic cultivation using an agro-industrial by-product stimulated the growth of all species, yielding high amounts of protein-rich biomass. The study also provided insights into the proteome of Chlorella cells cultured under molasses supplementation conditions.
Article
Chemistry, Multidisciplinary
Weichao Wang, Xian Liu, Changwen Zhang, Fei Sheng, Shanjun Song, Penghui Li, Shaoqing Dai, Bin Wang, Dawei Lu, Luyao Zhang, Xuezhi Yang, Zhihong Zhang, Sijin Liu, Aiqian Zhang, Qian Liu, Guibin Jiang
Summary: Scientists have discovered a significant variation in the ratio of copper isotopes in the blood of bladder cancer patients, providing new insights into the molecular mechanisms of copper imbalance. They have also developed a machine learning model to classify bladder cancer based on copper isotopes and concentration, with high diagnostic capability.
Article
Agriculture, Dairy & Animal Science
Federico Bonsembiante, Alessia Giordano, Claudia Gili, Sandro Mazzariol, Michele Berlanda, Carlo Guglielmini, Silvia Bedin, Maria Elena Gelain
Summary: Serum protein electrophoresis (SPE) is a widely used method to determine the concentration of serum protein fractions. In this study, we established reference intervals for serum protein fractions in bottlenose dolphins under human care, which may be useful for clinicians in interpreting laboratory data.
Article
Ophthalmology
Somnath De, Sandeep Saxena, Apjit Kaur, Abbas A. Mahdi, Arvind Misra, Malvika Singh, Carsten H. Meyer, Levent Akduman
Summary: The study showed that anti-VEGF therapy restores the barrier effect of ELM, causing ELM to restore first followed by EZ restoration in diabetic macular oedema (DMO).
Article
Orthopedics
Varsha Shukla, Siddharth Kumar Das, Abbas Ali Mahdi, Shweta Agarwal, Ragini Alok, Jamal Akhtar Ansari, Sukhanshi Khandpur
Summary: This study found that levels of oxidative stress markers were significantly higher in FMS patients, while essential metals were significantly lower, and these were closely related to the severity of the disease.
JOURNAL OF BACK AND MUSCULOSKELETAL REHABILITATION
(2021)
Article
Biochemistry & Molecular Biology
Jyoti Yadav, Anoop Kumar Verma, Md. Kaleem Ahmad, Ravindra K. Garg, Shiuli, Abbas Ali Mahdi, Shrikant Srivastava
Summary: Alzheimer's disease patients showed higher levels of non-essential metals and lower levels of essential biometals in their blood. Up-regulation of target genes was observed in AD patients, but there was no significant correlation found between metals dyshomeostasis and gene expressions in this study.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Neurosciences
Mohammad Rumman, Shivani Pandey, Babita Singh, Mrinal Gupta, Saba Ubaid, Abbas Ali Mahdi
Summary: The study demonstrated that GE can ameliorate hypoxia-induced cognitive dysfunction in mice by reducing oxidative stress, suppressing neuroinflammation, and enhancing the expression of neuroprotective genes in the hippocampus.
NEUROTOXICITY RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Babita Singh, Shivani Pandey, Mohammad Rumman, Shashank Kumar, Prem Prakash Kushwaha, Rajesh Verma, Abbas Ali Mahdi
Summary: The study showed that Bacopa monnieri extract has neuroprotective and neurorescue effects against MPTP-induced degeneration of dopaminergic neurons, potentially slowing down disease progression and delaying neurodegeneration in Parkinson's disease.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohd Zahid, Pushkar Singh Rawat, Shalini Singh, Akshay Kumar Gupta, Rumana Ahmad, Abbas Ali Mahdi, Md Kaleem Ahmad, Sudhir Mehrotra
Summary: The study found that curcumin and quercetin have a beneficial impact on lead toxicity, possibly due to their antioxidant properties that can counteract the oxidative stress induced by lead exposure. Therefore, the combination of curcumin and quercetin may serve as a useful supplement in the appropriate management of lead poisoning.
INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Esha Sarkar, Aparna Misra, Abbas Ali Mahdi, Anchal Trivedi, Afreen Khan
Summary: Chemoresistance is a major obstacle in cancer treatment, and various strategies have been discovered to overcome this issue, such as novel agents, drug carriers, and combination treatment targeting different pathways simultaneously.
CURRENT DRUG THERAPY
(2021)
Article
Environmental Sciences
Naina Dwivedi, Abbas Ali Mahdi, Sujata Deo, Mohammad Kaleem Ahmad, Durgesh Kumar
Summary: The study aimed to assess the correlation between transplacental transfer of xenobiotics and resulting biochemical alterations in pregnant women in North India. The results showed higher organo-chlorine pesticides levels in preterm maternal blood, along with increased genotoxicity and oxidative stress effects. The findings suggest a potential link between pesticides exposure and adverse pregnancy outcomes, highlighting the importance of early detection and stricter guidelines for pesticide use.
ENVIRONMENTAL RESEARCH
(2022)
Article
Genetics & Heredity
Ravi Shanker Yadav, Surya Kant, Prashant Mani Tripathi, Anumesh K. Pathak, Abbas Ali Mahdi
Summary: The study found that mRNA levels of IL-1 beta, IL-8, and NF-kappa B were significantly higher in COPD patients compared to healthy controls; smokers with COPD had significantly higher mRNA expression levels of IL-1 beta, IL-8, and NF-kappa B than non-smokers; increasing pack-years of smoking correlated with worsening COPD severity.
Article
Biochemistry & Molecular Biology
Abbas Ali Mahdi, Jamal Akhtar Ansari, Priyanka Chaurasia, Mohammad Kaleem Ahmad, Shipra Kunwar, Sally McClean, Pratheepan Yogarajah
Summary: This study investigated the blood lead levels in pregnant women and umbilical cord blood, finding elevated lead levels in both groups. There was a strong positive correlation between the maternal and umbilical cord blood lead levels. Factors such as recent home painting and close proximity to traffic congestion were significantly associated with higher maternal blood lead levels. Education, mother age, fuel, and water sources did not show a significant association. Screening of blood lead levels and stricter regulations on lead-based products are recommended to reduce the exposure to lead in high-risk women and the general population.
INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY
(2023)
Article
Environmental Sciences
Naina Dwivedi, Abbas Ali Mahdi, Sujata Deo
Summary: The present study aimed to assess the safety of infants in North India in relation to the presence of persistent organochlorine pesticides (OCPs) in breast milk, which could transfer endocrine-disrupting chemicals to newborns. Samples of colostrum and breast milk were collected and analyzed for pesticide concentration. Results showed that OCPs concentration was higher in breast milk compared to colostrum, indicating an increase in pesticides contamination over lactation. The study also found that women consuming non-vegetarian food had higher exposure to OCPs, and factors such as mother's age, gestational age, and infant birth weight were associated with OCPs levels in colostrum and breast milk. The study highlights the need for regulation and further research on the relationship between pesticide residues in breast milk and maternal and child health.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ajay Kumar Verma, Anuj Kumar Pandey, Arpita Singh, Surya Kant, Abbas Ali Mahdi, Ved Prakash, Kausar Mahmood Ansari, Rakesh Kumar Dixit, Shyam Chand Chaudhary
Summary: This study found that serum levels of MMP-9, COX-2, and PGE-2 are correlated with GOLD grade, CAT score, and clinical history in the COPD group, serving as potential indicators for understanding disease progression.
INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Shalini Gupta, Kumud Nigam, Ratnesh Kumar Srivastav, Md. Kaleem Ahmad, Abbas Ali Mahdi, Somali Sanyal
Summary: The study found a significant association between SNPs of TNF-alpha and TNF-beta genes with oral pre-cancerous lesions and oral squamous cell carcinoma. Specifically, the GA genotype of TNF-alpha and the AG heterozygote of TNF-beta were associated with an increased risk of oral diseases.
ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG
(2022)
Article
Biochemistry & Molecular Biology
Raza Abbas Mahdi, Israr Ahmad, Talha Jilani, Jamal Akhtar Ansari, Farzana Mahdi, Abbas Ali Mahdi, Tabrez Jafar
Summary: The study investigated dopamine gene polymorphism, biochemical and oxidative stress parameters in elderly patients with and without depression. Changes in levels of various markers including dopamine, antioxidants and oxidative stress markers were observed in depressed individuals compared to controls, suggesting a possible association between these factors and depression in the aging population.
INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Sonal Sachan, Sucheta Pathania, Abbas Ali Mahdi, Swastika Suvirya, Atin Singhai
Summary: Long-term ingestion of high concentrations of arsenic can lead to arsenicosis, which is common in India and Bangladesh due to contamination of groundwater with naturally occurring high concentrations of arsenic in the earth's crust. Skin and internal organ involvement in arsenic endemic regions should be given special attention.
JOURNAL OF HEALTH AND POLLUTION
(2021)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)
