Journal
CLINICA CHIMICA ACTA
Volume 425, Issue -, Pages 169-175Publisher
ELSEVIER
DOI: 10.1016/j.cca.2013.07.023
Keywords
Circulating DNA; Cancer epigenetics; Blood-processing protocols; Quantification; DNA methylation; Methylation specific PCR
Categories
Funding
- National Institutes of Health [R01CA155305, U54CA151838, R21CA173390]
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The use of methylated tumor-specific circulating DNA has shown great promise as a potential cancer biomarker. Nonetheless, the relative scarcity of tumor-specific circulating DNA presents a challenge for traditional DNA extraction and processing techniques. Here we demonstrate a single tube extraction and processing technique dubbed methylation on beads that allows for DNA extraction and bisulfite conversion for up to 2 ml of plasma or serum. In comparison to traditional techniques including phenol chloroform and alcohol extraction, methylation on beads yields a 15- to 5-fold improvement in extraction efficiency. The technique results in far less carryover of PCR inhibitors yielding analytical sensitivity improvements of over 25-fold. The combination of improved recovery and sensitivity make possible the detection of rare epigenetic events and the development of high sensitivity epigenetic diagnostic assays. (C) 2013 Elsevier B.V. All rights reserved.
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