Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Andreea Spiridon, Eva Oburger, Younes Valadbeigi, Tobias Kloeimbock, Christian Stanetty, Nicolas Kratena, Markus Draskovits, Tim Causon, Stephan Hann
Summary: Phytosiderophores (PS) released by grass species play a crucial role in iron plant nutrition. This study presents comprehensive methods to fully characterize and quantify all eight naturally occurring PS belonging to the mugineic acid family. These newly developed methods can be applied to improve crop yield and micronutrient grain content for food consumption through plant-based biofortification.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agricultural Engineering
Zainab Syed, Monika Sogani, Anu Kumar, Jayana Rajvanshi, Gopesh Sharma, Kumar Sonu
Summary: This research focused on the biodegradation of synthetic estrogenic compound 17 alpha-ethinylestradiol (EE2) and compared its degradation efficiency and power output in suspension flasks and a Bioelectrochemical System (BES). The results showed that the BES had significantly higher EE2 removal rate and power density. The study also identified a potential pathway for EE2 degradation using the newly isolated strain Rhodopseudomonas palustris MDOC01, and detected important metabolites during the bioelectrochemical treatment process of EE2.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Maria Teresa Fern, Ana Maria Casas-Ferreira, Encarnacion Rodriguez-Gonzalo, Bernardo Moreno-Cordero, Jose Luis Perez-Pavon
Summary: A method utilizing gC-MS/MS for the determination of proteinogenic amino acids in urine samples was proposed and validated with LC-MS/MS using synthetic urine. Both methods showed reliable results in the quantification of individual amino acids. Additionally, no significant differences in amino acid concentrations related to sex were observed in the evaluated compounds.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Ya-Ling Yeh, Sheng-Meng Wang
Summary: Synthetic cathinones are widely used in Taiwan as new psychoactive substances, especially eutylone. This study developed a method to identify and quantify eutylone and its metabolites in urine samples. The researchers conducted in vitro metabolism experiments and identified several metabolites. Based on the results, they proposed the most appropriate biomarkers for eutylone consumption. These findings expand the detection window for eutylone and provide valuable information for toxicology research.
DRUG TESTING AND ANALYSIS
(2022)
Review
Chemistry, Analytical
Stian Kogler, Kristina Saeterdal Komurcu, Christine Olsen, Jun-ya Shoji, Froydis Sved Skottvoll, Stefan Krauss, Steven Ray Wilson, Hanne Roberg-Larsen
Summary: Organoids and organ-on-a-chip devices are important tools for studying various aspects of organs. This review focuses on the application of mass spectrometry in studying organoids and organ-on-a-chip-derived material. It discusses the use of liquid chromatography-mass spectrometry (LC-MS) for proteomics, metabolomics/lipidomics, and hormones, as well as mass spectrometry imaging (MSI) of organoids. The analysis has provided insights into the development and disease of organs, potentially replacing or complementing animal models.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Biochemical Research Methods
Steven J. Lehotay
Summary: UHPLC coupled with MS/MS is a powerful tool for multiclass, multiresidue analysis. The study validated quantification and identification of 169 veterinary drugs in eggs, recommending monitoring >2 ion transitions per analyte for improved analysis with MS/MS.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Physics, Atomic, Molecular & Chemical
Jay S. Bhanot, Kimberly C. Fabijanczuk, Abdirahman M. Abdillahi, Hsi-Chun Chao, Nicolas J. Pizzala, Frank A. Londry, Eric T. Dziekonski, James W. Hager, Scott A. McLuckey
Summary: A commercial quadrupole/time-of-flight tandem mass spectrometer has been modified and evaluated for its performance in conducting ion/ion reaction studies involving high mass (>100 kDa) ions. The modifications enable resonance excitation of ions, selective ion isolation, and ion parking, providing a platform for the study of high mass ion/ion reactions.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Fan Huang, Ziying Wang, Lulu Wang, Ruxia Liu, Chi Ma, Yanzhong Che, Yiwen He, Ji Ye
Summary: This study comprehensively investigated the chemical ingredients and metabolites of Lingbao Huxin Dan (LBHX) using advanced analytical techniques. The researchers identified 53 volatile compounds and 191 non-volatile chemical components in LBHX. In addition, potential active compounds targeting proteins associated with sick sinus syndrome were identified. This study provides important insights for further understanding the mechanism of LBHX in treating cardiovascular diseases.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Prasad Phapale, Andrew Palmer, Rose Muthoni Gathungu, Dipali Kale, Britta Brugger, Theodore Alexandrov
Summary: EMBL-MCF is an open LC-MS/MS spectral library with over 1600 fragmentation spectra from 435 authentic standards of endogenous metabolites and lipids. It features chromatographic profiles acquired with different LC-MS methods and coverage of different adduct ions. The library contains many biologically important metabolites and unique metabolites and lipids compared to other public libraries.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Applied
Dan Qian, Juan Xi, Yinglan Nie, Mailing Zou, Jiao Zhang, Saleh Alseekh, Alisdair R. Fernie, Jinlong Chen, Luqi Huang
Summary: In this study, a comprehensive characterization and quantitative analysis of dicaffeoyl polyamine derivatives (DCPAds) in goji berries were conducted using liquid chromatography-mass spectrometry. A strategy was developed to determine the fragmentation patterns of authentic standards, and three diagnostic ions were used to quickly identify these compounds in goji berries. The natural variation and differential spatiotemporal accumulation patterns of seven DCPAds in 94 goji berry batches were revealed. Moreover, principal component analysis of metabolomics data grouped the wild and cultivated samples, and distinguished green samples and those at other developmental stages. These findings provide essential data for further phytochemical studies, metabolite identification, and quality control of goji berries.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Lanxin Wang, Huizheng Fu, Junmao Li, Linan Chen, Jiaxi Yang, Lan Zhong, Xiaowu Xiao, Yulin Feng, Yuehua Luo
Summary: This study established a systematic method to classify and identify the chemical constituents of Fufang Xianzhuli Ye (FXZL) using ultra-high-performance liquid chromatography and mass spectrometry. A total of 106 compounds were identified in FXZL, providing valuable information for quality control and further exploration of its active ingredients.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Environmental Sciences
Patricia Aguilar-Alarcon, Susana Gonzalez, Alexandros G. Asimakopoulos, Oyvind Mikkelsen
Summary: This study used quadrupole time-of-flight mass spectrometry (QTOF-MS) to characterize the molecular fingerprints of fulvic acids (RASFA) in recirculating aquaculture systems (RAS) and compared them with reference standards. The results demonstrated that QTOF-MS is a rapid and comprehensive screening technique for characterizing and monitoring differences in fulvic acid composition in RAS.
Article
Chemistry, Analytical
Takaaki Matsumoto, Wataru Yamazaki, Akihiro Jo, Shoujiro Ogawa, Kuniko Mitamura, Shigeo Ikegawa, Tatsuya Higashi
ANALYTICAL SCIENCES
(2018)
Article
Chemistry, Applied
Takashi Yarita, Shinsuke Inagaki, Taichi Yamazaki, Migaku Kawaguchi, Hajime Uchida, Akiko Takatsu, Toshiyuki Suzuki
Article
Biochemical Research Methods
Yusuke Yoshimura, Ryuichiro Hibi, Akiho Nakata, Moeka Togashi, Shoujiro Ogawa, Takayuki Ishige, Mamoru Satoh, Fumio Nomura, Tatsuya Higashi
BIOMEDICAL CHROMATOGRAPHY
(2019)
Article
Instruments & Instrumentation
Shinsuke Inagaki, Toshihiro Suzuki, Toshiaki Asakai, Masahiko Numata, Nobuyasu Hanari, Kouji Kitanaka, Masaharu Hagiwara, Shuichi Kotaki
Article
Chemistry, Analytical
Yukiko Horie, Ayaka Goto, Sumi Tsubuku, Mari Itoh, Shigeo Ikegawa, Shoujiro Ogawa, Tatsuya Higashi
ANALYTICAL SCIENCES
(2019)
Review
Pharmacology & Pharmacy
Masanobu Nishidate, Mitsuhiro Hayashi, Hiroaki Aikawa, Kouji Tanaka, Naoyuki Nakada, Shin-ichi Miura, Shoraku Ryu, Tatsuya Higashi, Yoshinori Ikarashi, Yasuhiro Fujiwara, Akinobu Hamada
DRUG METABOLISM AND PHARMACOKINETICS
(2019)
Article
Chemistry, Analytical
Takaaki Matsumoto, Maho Sato, Wataru Yamazaki, Shoujiro Ogawa, Tatsuya Higashi
MICROCHEMICAL JOURNAL
(2019)
Article
Chemistry, Analytical
Emi Takeo, Yuki Sugiura, Tatsuki Uemura, Koshiro Nishimoto, Masanori Yasuda, Eiji Sugiyama, Sumio Ohtsuki, Tatsuya Higashi, Tetsuo Nishikawa, Makoto Suematsu, Eiichiro Fukusaki, Shuichi Shimma
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Masahiro Nakamura, Marina Moritsuna, Keita Yuda, Shunsuke Fujimura, Yuki Sugiura, Shuichi Shimma, Koshiro Nishimoto, Tetsuo Nishikawa, Makoto Suematsu, Shoujiro Ogawa, Tatsuya Higashi
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Food Science & Technology
Yukiko Horie, Ayaka Goto, Riko Imamura, Mari Itoh, Shigeo Ikegawa, Shoujiro Ogawa, Tatsuya Higashi
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Tsuyoshi Murai, Shigeki Jin, Mari Itoh, Yukiko Horie, Tatsuya Higashi, Shigeo Ikegawa
Article
Pharmacology & Pharmacy
Kazumi Yoshizawa, Kozue Nakashima, Mizuho Tabuchi, Ayano Okumura, Yuko Nakatake, Mitsuhiko Yamada, Yayoi Tsuneoka, Tatsuya Higashi
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2020)
Article
Biochemical Research Methods
Miki Kamemura, Mai Yokota, Shoujiro Ogawa, Takahiro Sugiura, Tatsuya Higashi
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2020)
Article
Chemistry, Analytical
Akihiro Jo, Sae Koyagi, Wataru Hobo, Saori Otani, Shoujiro Ogawa, Tatsuya Higashi
ANALYTICAL SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Yusuke Yoshimura, Moeka Togashi, Shoujiro Ogawa, Tatsuya Higashi
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)