4.5 Article

Detection of Marteilia refringens infecting the European flat oyster Ostrea edulis and the dwarf oyster Ostrea stentina in southern Portugal and Spain

Journal

JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 130, Issue -, Pages 52-55

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2015.07.003

Keywords

Marteilia refringens; Ostrea edulis; Ostrea stentina; Histology; PCR; In situ hybridization

Categories

Funding

  1. project(Operational Programme for Cross-border Cooperation between Spain and Portugal) [0433_BONAQUA_5_E POCTEP 2007-2013]
  2. European Regional Development Fund, European Union (FEDER, UE)
  3. project HERPEMOL (JACUMAR)
  4. PROSANI [31-03-01-FEP-002]
  5. IFAPA
  6. European Union FEDER program

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In the present study, Marteilia sp. was detected by histological examination and in situ hybridisation in Ostrea edulis and Ostrea stentina collected in southern Iberian Peninsula. Marteilia refringens DNA was detected by PCR in O. edulis (collected in southern Portugal) and O. stentina (collected in southern Spain and Portugal). Sequencing analysis revealed the presence of M. refringens type O in O. edulis, and type O and M in O. stentina. This is the first confirmed detection of M. refringens in Portugal and the first report on the occurrence of M. refringens infecting O. stentina in Europe. (C) 2015 Elsevier Inc. All rights reserved.

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