Review
Cardiac & Cardiovascular Systems
Ya-Fei Li, Ya-Xin Wang, Hao Wang, Yao Ma, Lian-Sheng Wang
Summary: Heart failure following ischemic heart disease is a leading cause of death worldwide, and cardiac regeneration aims to promote cardiomyocyte proliferation. Recent research has focused on the role of protein posttranslational modifications in cardiac regeneration, with a particular emphasis on their effects on cardiomyocyte proliferation.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Keith E. Keenan, Derek K. Zachman, Matthew D. Hirschey
Summary: Protein modifications play a significant role in cell biology across various organisms. Discoveries in the early 20th century challenged traditional definitions of amino acids, and the advent of mass-spectrometry-based proteomics in the latter part led to a rapid increase in known protein modifications, with over 500 identified today. New computational tools in data science, machine learning, and artificial intelligence are expected to further advance the discovery and understanding of protein modifications in the future.
Article
Biochemistry & Molecular Biology
Annika Frauenstein, Stefan Ebner, Fynn M. Hansen, Ankit Sinha, Kshiti Phulphagar, Kirby Swatek, Daniel Hornburg, Matthias Mann, Felix Meissner
Summary: The study introduces a new experimental method (MIP-APMS) to explore the composition, interactions, and functional phenotypes of protein communities within cells. It identifies previously undescribed modifications and interactions in monocytes, revealing regulatory mechanisms of protein complexes in the immune system. Additionally, distinct mechanisms of action for small molecule inhibitors of p38 (MAPK14) are uncovered.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zongmin Li, Yaqian Huang, Boyang Lv, Junbao Du, Jing Yang, Ling Fu, Hongfang Jin
Summary: Gasotransmitters participate in various cellular processes via oxidative posttranslational modifications of specific cysteines. Recent advances have clarified the mechanisms and biological functions of these modifications. Determining the sites of these modifications is crucial for understanding biology.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemical Research Methods
Yanzheng Meng, Lin Zhang, Laizhi Zhang, Ziyu Wang, Xuanwen Wang, Chan Li, Yu Chen, Shipeng Shang, Lei Li
Summary: CysModDB is an integrated platform designed to facilitate the study of cysteine posttranslational modifications (PTMs). It brings together comprehensive CysPTM resources and analysis tools, providing researchers with online computational and analytical tools for CysPTM site prediction, gene enrichment, and sequence feature investigation.
BRIEFINGS IN BIOINFORMATICS
(2022)
Review
Biochemistry & Molecular Biology
Piengtawan Tappiban, Yining Ying, Feifei Xu, Jinsong Bao
Summary: The study focuses on the proteomics and posttranslational modifications (PTMs) of starch biosynthesis-related proteins in rice endosperm, highlighting the differential expression of these proteins in developing seeds. Most proteins involved in starch biosynthesis increase at 6-20 days after flowering and decrease under high-temperature conditions. Various PTMs, including phosphorylation, lysine acetylation, succinylation, lysine 2-hydroxyisobutyrylation, and malonylation, target starch biosynthesis proteins in rice seeds. Further research on the role of these PTMs in starch biosynthesis is ongoing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Rotimi Fasimoye, Wentao Dong, Raja S. Nirujogi, Eshaan S. Rawat, Miharu Iguchi, Kwamina Nyame, Toan K. Phung, Enrico Bagnoli, Alan R. Prescott, Dario R. Alessi, Monther Abu-Remaileh
Summary: The Golgi is a crucial organelle involved in protein and lipid biosynthesis, as well as sorting and secretion. Dysfunctional Golgi is associated with various diseases. This study presents a Golgi immunoprecipitation technique to isolate intact Golgi mini-stacks for analysis. The analysis pipeline reveals the molecular features of the human Golgi, including proteome, metabolome, and lipidome profiles. This comprehensive molecular map provides a powerful method to study the Golgi in health and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Endocrinology & Metabolism
Huimei Wei, Peng Huo, Shun Liu, Hua Huang, Shun Zhang
Summary: PCOS is a lifelong disorder affecting a significant number of women and associated with increased risk of various diseases. Posttranslational modifications play a crucial role in PCOS development, and understanding their roles and mechanisms can provide insights for future research and treatment.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Devasahayam Arokia Balaya Rex, Jaikanth Chanderasekaran, Akhila Balakrishna Rai, Homen Phukan, Abhijit Sarma, Thottethodi Subrahmanya Keshava Prasad, Madathiparambil Gopalakrishnan Madanan
Summary: In this study, novel insights on the Leptospira proteome, posttranslational modifications, and pathogen-host interactions were obtained through a systems science approach. The authors identified 3693 unique high-confidence PTM sites and analyzed phosphorylated peptides in detail. Furthermore, the molecular docking results predicted druggable hotspots for the PTM-GroEL protein in its interaction with STAT3. These findings have potential applications in rational drug design and peptide engineering for the prevention and treatment of leptospirosis.
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Christine M. Loescher, Anastasia J. Hobbach, Wolfgang A. Linke
Summary: This review provides an overview of the changes in cardiac titin properties at a molecular level, including the role isoform diversity and post-translational modifications play in regulating myocardial function, and discusses the importance of this regulation imbalance in heart disease.
CARDIOVASCULAR RESEARCH
(2022)
Article
Multidisciplinary Sciences
Takeshi Tsusaka, Juan A. Oses-Prieto, Christina Lee, Brian C. DeFelice, Alma L. Burlingame, Emily L. Goldberg
Summary: Ketone bodies are produced in the liver during glucose scarcity and act as alternative energy sources. Beta-hydroxybutyrate (BHB) has been identified as a signaling molecule, and lysine beta-hydroxybutyrylation (Kbhb) as a newly discovered post-translational modification. Kbhb is metabolically sensitive and found on proteins in multiple cellular compartments, suggesting its importance in ketone body-regulated physiology. However, caution is needed when using the H3K9bhb antibody to interpret gene regulation data, as it may recognize other modifications besides beta-hydroxybutyrylation.
Article
Chemistry, Analytical
Francis Berthias, Dale A. Cooper-Shepherd, Frederik H. V. Holck, James I. Langridge, Ole N. Jensen
Summary: The combination of cyclic ion mobility spectrometry (cIM) and electron capture dissociation (ECD) allows for the complete separation and sequencing of large isomeric peptides, providing a new method for identifying biologically important proteoforms in complex mixtures.
ANALYTICAL CHEMISTRY
(2023)
Article
Cardiac & Cardiovascular Systems
Jana P. Lovell, Kevin Bermea, Jinsheng Yu, Sylvie Rousseau, Charles D. Cohen, Aashik Bhalodia, Marcelle Dina Zita, Richard D. Head, Roger S. Blumenthal, Rami Alharethi, Julie Damp, John Boehmer, Jeffrey Alexis, Dennis M. Mcnamara, Garima Sharma, Luigi Adamo
Summary: This study used serum proteomic analysis to characterize the pathophysiology of peripartum cardiomyopathy (PPCM). It found that PPCM is a distinct disease entity that is characterized by dysregulation of inflammation-related pathways and cholesterol metabolism-related anti-inflammatory pathways.
JACC-HEART FAILURE
(2023)
Review
Pharmacology & Pharmacy
Abhi Dutta, Shubham Saha, Ajay Bahl, Anupam Mittal, Trayambak Basak
Summary: Cardiorenal syndrome is a broad spectrum disorder affecting the heart and kidneys. India has a high burden of acute cardiorenal syndrome, with 46.1% of patients diagnosed in India. Acute cardiorenal syndrome involves sudden kidney deterioration in acute heart failure patients. The pathophysiology includes hyperactivation of the sympathetic nervous system and the renin-angiotensin-aldosterone system. Complications increase the risk of mortality, making it a global healthcare burden. Current biomarkers have limitations in early detection, highlighting the need for novel proteomic biomarkers to prevent progression.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Akila Wijerathna-Yapa, Elke Stroeher, Ricarda Fenske, Lei Li, Owen Duncan, A. Harvey Millar
Summary: Autophagy is a catabolic process that plays a crucial role in degrading cytoplasmic proteins and organelles in a lysosome or vacuole-dependent manner. Proteomic studies have helped uncover the protein regulatory mechanisms of autophagy, including the identification of receptor and cargo proteins and protein-protein interactions.
JOURNAL OF PROTEOME RESEARCH
(2021)