Journal
JOURNAL OF INSECT PHYSIOLOGY
Volume 81, Issue -, Pages 81-89Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jinsphys.2015.07.007
Keywords
Lectin; Dioclea violacea; Anagasta kuehniella; Bioinsecticides; Enzyme activity; Insect gut
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Funding
- Capes (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- FUNDECT (Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado do Mato Grosso do Sul)
- FINEP (Financiamento de Estudos e Projetos/Ministerio da Ciencia e Tecnologia)
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Entomotoxic plant lectins have been extensively studied in the past two decades, yet the exact mechanisms underlying their toxic effects remain unknown. This study investigated the effects of Dioclea violacea lectin (DVL) on larval development in Anagasta kuehniella. Chronic exposure of larvae (from neonates to the fourth instar) demonstrated that DVL interfered with larval growth, retarding development and decreasing larval mass without affecting survival. DVL decreased trypsin-like, chymotrypsin-like, and a-amylase activities and proved resistant to proteolysis by midgut proteases up to 24 h. Shorter exposures to dietary DVL had no effect on midgut enzyme activity. Feeding fourth-instar larvae with fluorescently-labeled DVL revealed lectin binding to the peritrophic membrane. (C) 2015 Elsevier Ltd. All rights reserved.
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