Journal
CHROMATOGRAPHIA
Volume 77, Issue 13-14, Pages 957-961Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-014-2707-8
Keywords
Polyvinylidene fluoride (PVDF); trans-Resveratrol; trans-Epsilon-viniferin; Vitis vinifera; Tyrosinase inhibition
Funding
- Centre National de la Recherche Scientifique
- University of Montpellier
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Repeated chromatographic analyses of polyphenolic vine stalks extracts allowed us to note a huge adsorption difference on polyvinylidene fluoride (PVDF), between trans-resveratrol 1 and (+)-trans-epsilon-viniferin 2. We could optimize the conditions (solvent, saturation of the process), for this polymer to adsorb very selectively 2, with regard to the monomer 1 that remains in solution. Since membrane filters made of PVDF are quite often used for HPLC samples filtration, this observation prompted us to inform phytochemists studying plant stilbenoid contents. Based on this background information, we developed a straightforward and inexpensive enrichment process for either 1 and/or 2, from crude Vitis vinifera stalks extracts, allowing to get them in a pure form. Having at hand large amounts of these two pure compounds, they were tested and compared to a set of other relevant molecules for some biological properties: trans-epsilon-viniferin 2 was shown to be the most powerful tyrosinase inhibitor, among all samples tested.
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