Article
Chemistry, Applied
Ting Zeng, Baohua Fang, Fenglin Huang, Li Dai, Zhi Tang, Jinglin Tian, Guodong Cao, Xuanlin Meng, Yuanchen Liu, Bo Lei, Minghua Lu, Zongwei Cai
Summary: Through metabolomics analysis, it was found that under cadmium stress, most carbohydrates and amino acids were down-regulated as the cadmium concentration increased in rice grains, except for myoinositol which can prevent cadmium toxicity. D-Mannitol and L-cysteine were up-regulated in low-cadmium-accumulating rice with the increase of cadmium concentration. Organic acids, especially 13-(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid related to alphalinolenic acid metabolism and jasmonic acid production, were activated.
Article
Plant Sciences
Meryem Slighoua, Mohamed Chebaibi, Ismail Mahdi, Fatima Ez-zahra Amrati, Raffaele Conte, Mary Anne W. Cordero, Amal Alotaibi, Hamza Saghrouchni, Abdelkrim Agour, Touria Zair, Amina Bari, Dalila Bousta
Summary: In this study, the chemical composition of lavender plant extracts was analyzed and their analgesic and wound-healing effects were evaluated. The extracts showed significant analgesic and healing activities, indicating their potential as natural sources for pharmaceutical applications.
Article
Multidisciplinary Sciences
Amal A. Maamoun, Radwa H. El-akkad, Mohamed A. Farag
Summary: The study compared the metabolite profiles of young fruit and mature fruit of Luffa aegyptiaca, revealing significant differences in chemical composition. Young fruit was found to be enriched in metabolites, while mature fruit predominated in fatty acids. Additionally, several oleanene triterpene glycosides were more abundant in young fruit.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Meryem Slighoua, Ismail Mahdi, Fatima Zahrae Moussaid, Omkulthom Al Kamaly, Fatima Ez-zahra Amrati, Raffaele Conte, Aziz Drioiche, Asmaa Saleh, Abdelilah Iraqi Housseini, Amina Bari, Dalila Bousta
Summary: This study investigated the phytochemical composition and bioactivities of parsley, including antioxidant, analgesic, antimicrobial, and wound healing effects. The results revealed that parsley contains polyphenols with strong antimicrobial and antioxidant activities, and also exhibits significant analgesic and wound healing effects.
Article
Agriculture, Multidisciplinary
Bo-Min Kim, Hyeon-Jeong Lee, Yeong H. Song, Hyun-Jin Kim
Summary: Increasing NaCl concentration led to decreased growth and mineral imbalance in spinach plants. NaCl irrigation resulted in decreased levels of most metabolites, affecting the nutritional quality of spinach.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Food Science & Technology
Satoru Tomita, Masaru Nomura, Yousuke Arakawa, Takayuki Miura, Sora Hayashida, Tatsuro Hagi, Miho Kobayashi, Satoshi Suzuki, Hideyuki Yamashita, Kaoru Sato, Ken-Ichi Kusumoto
Summary: This study investigated the impact of traditional koji molds on the chemical characteristics of soft-type natural cheese. The results showed significant differences in metabolite profiles between koji cheese and other mold-ripened cheeses, indicating the key compositional difference derived from cheese ripening with Aspergillus strains. The findings could contribute to the quality improvements of koji cheese products.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Pufan Zheng, Mei Zhang, Xin Fang, Lili Tang, Zhixue Wang, Fuchen Shi
Summary: This study identified and quantified metabolites in three pear species using a targeted LC-MS/MS approach. The results showed distinct differences in metabolite composition between cultivars, and identified metabolic pathways related to fruit quality. D-xylose, formononetin, procyanidin A1 and beta-nicotinamide mononucleotide were identified as major differentially expressed metabolites.
Article
Chemistry, Applied
Shiqi Liao, Jinlin Han, Chunyan Jiang, Binbin Zhou, Zhenju Jiang, Jie Tang, Wenwu Ding, Zhenming Che, Hongbin Lin
Summary: With the use of a thermostatic fermenter, this study identified and analyzed the physicochemical factors and volatile metabolites of fermented broad beans. Through the application of HS-SPME-GC x GC-MS, the volatile compounds in fermented broad beans were detected, and metabolomics was used to explore the physicochemical characteristics and analyze possible metabolic mechanisms. A total of 184 different metabolites were detected, and they were annotated to various metabolic pathways of carbohydrate and amino acid metabolism. This study provides references for improving the quality of tank-fermented broad beans and upgrading the Pixian broad bean paste industry.
Article
Forestry
Nai-Wen Tsao, Yen-Chi Lin, Yen-Hsueh Tseng, Shih-Chang Chien, Sheng-Yang Wang
Summary: This study identified the main compounds of exudates from 13 conifers in Taiwan, revealing that the main volatiles were alpha-pinene, beta-ocimene, beta-pinene, sabinene, and caryophyllene. It also found that the nonvolatile compounds were diterpenoids, classified into three types. The analysis results of conifers' epigenetics by GC-MS and HSQC were similar to traditional taxonomy classification, indicating the usefulness of this technology in classifying conifers. Additionally, certain conifers showed strong antifungal activity against white-rot and brown-rot fungi.
JOURNAL OF WOOD SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Stefan Ivanovic, Katarina Simic, Vele Tesevic, Ljubodrag Vujisic, Marko Ljekocevic, Dejan Godevac
Summary: This study investigated the main volatiles and aroma compounds of non-aged plum brandies originating from different plum cultivars and fermented using different conditions, and applied multivariate data analysis to reveal the differences in chemical profiles. The correlation between the chemical composition and sensory characteristics of plum brandies was also performed. The results confirmed the potential of GC-FID-MS combined with multivariate data analysis in assessing the quality of plum brandy.
Article
Multidisciplinary Sciences
Ding Wang, Jing Zhu, Na Li, Hongyang Lu, Yun Gao, Lei Zhuang, Zhongjian Chen, Weimin Mao
Summary: This study analyzed the plasma metabolic profile of patients with malignant mesothelioma and identified differential metabolites and disrupted metabolic pathways. Five potential plasma biomarkers were identified, which could be critical for the diagnosis of malignant mesothelioma.
Article
Chemistry, Multidisciplinary
Murugan Prasathkumar, Kannan Raja, Krishnan Vasanth, Ameer Khusro, Subramaniam Sadhasivam, Muhammad Umar Khayam Sahibzada, Mohamed Ragab Abdel Gawwad, Dunia A. Al Farraj, Mohamed S. Elshikh
Summary: The study determined the phytochemical components and various bioactive properties of Senna auriculata (L.) Roxb. leaves extract, demonstrating its potential as an ideal agent for antibacterial, antioxidant, anti-inflammatory, anti-diabetic, and wound healing purposes in the future.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Food Science & Technology
Rajender Kumar, Dinesh Kumar
Summary: Allium is widely consumed as a spice around the world. This study compared the metabolome and antioxidant activity of different tissues of Allium cepa, A. sativum, and A. semenovii. The results showed that A. cepa and A. semenovii had higher antioxidant activity and polyphenol content compared to A. sativum. Additionally, various metabolites including polyphenols and sulfur containing compounds were identified. This study highlights the potential of A. semenovii for utilization in food and nutraceuticals.
FOOD RESEARCH INTERNATIONAL
(2023)
Review
Biochemical Research Methods
Deyber Arley Vargas Medina, Edvaldo Vasconcelos Soares Maciel, Natalia Gabrielly Pereira dos Santos, Fernando Mauro Lancas
Summary: Targeted and untargeted analysis are important methods in omics sciences. GC-MS is commonly used for volatile and thermally stable compounds, while LC-MS is used for compounds that cannot be analyzed by GC without chemical derivatization. Researchers are working on bridging the gap between LC and GC-MS techniques.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Agronomy
Rajaprakasam Sudhagar, Shanmugavel Priyanka, Vanniarajan Chockalingam, Vaithiyanathan Sendhilvel, Jegadeesan Souframanien, Kalimuthu Raja, Selvaraju Kanagarajan
Summary: This study explored the effects of powdery mildew on horse gram growth and yield through screening mutants and conducting metabolomic analysis. The research found differences in metabolite expression among different mutants and the accumulation of certain compounds related to disease resistance.
Article
Chemistry, Medicinal
Malwina Krause, Henryk Foks, Dagmara Ziembicka, Ewa Augustynowicz-Kopec, Agnieszka Glogowska, Izabela Korona-Glowniak, Krzysztof Bojanowski, Danuta Siluk, Katarzyna Gobis
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Medicine, General & Internal
Joanna Renke, Eliza Wasilewska, Sabina Kedzierska-Mieszkowska, Katarzyna Zorena, Sylwia Baranska, Tomasz Wenta, Anna Liberek, Danuta Siluk, Dorota Zurawa-Janicka, Aleksandra Szczepankiewicz, Marcin Renke, Barbara Lipinska
MEDICINA-LITHUANIA
(2020)
Article
Biochemical Research Methods
Wiktoria Struck-Lewicka, Renata Wawrzyniak, Malgorzata Artymowicz, Marta Kordalewska, Marcin Markuszewski, Marcin Matuszewski, Piotr Gutknecht, Janusz Siebert, Michal Jan Markuszewski
JOURNAL OF BREATH RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Kamil Luczykowski, Natalia Warmuzinska, Sylwia Operacz, Iga Stryjak, Joanna Bogusiewicz, Julia Jacyna, Renata Wawrzyniak, Wiktoria Struck-Lewicka, Michal J. Markuszewski, Barbara Bojko
Summary: This study introduces untargeted metabolomic profiling of biological fluids as a tool for the discovery of bladder cancer biomarkers. The analysis of metabolites contributing to differences could support a better understanding of bladder cancer development mechanisms.
Article
Biochemistry & Molecular Biology
Szymon Macioszek, Renata Wawrzyniak, Anna Kranz, Marta Kordalewska, Wiktoria Struck-Lewicka, Danuta Dudzik, Margot Biesemans, Michal Maternik, Aleksandra M. Zurowska, Michal J. Markuszewski
Summary: Renal dysplasia is a severe congenital abnormality of the kidney parenchyma, often leading to end-stage renal failure in childhood and early adulthood. Metabolomics studies have revealed alterations in fatty acid oxidation, amino acid, and purine metabolisms in children with renal dysplasia, offering potential early diagnostic indicators.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Malgorzata Artymowicz, Szymon Macioszek, Julia Jacyna, Joanna Dawidowska, Wiktoria Struck-Lewicka, Micha l J. Markuszewski, Danuta Siluk
Summary: This study aimed to develop a sample preparation procedure for the simultaneous extraction of 12 selected modified nucleosides and deoxynucleosides using solid phase extraction (SPE) technique. The developed and optimized SPE procedure allowed for successful extraction with a mean recovery of 76.0%, and was applied to urine samples using high performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-QqQ/MS).
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Review
Chemistry, Analytical
Danuta Dudzik, Szymon Macioszek, Wiktoria Struck-Lewicka, Marta Kordalewska, Magdalena Buszewska-Forajta, Malgorzata Waszczuk-Jankowska, Renata Wawrzyniak, Malgorzata Artymowicz, Joanna Raczak-Gutknecht, Danuta Siluk, Michal J. Markuszewski
Summary: The discovery of extracellular vesicles has opened up new possibilities in studying cell-to-cell communication, with potential therapeutic and diagnostic applications. However, challenges in isolating and analyzing these vesicles hinder their clinical utility, highlighting the importance of advanced analytical techniques like mass spectrometry-based metabolomics for understanding their molecular profiles.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Jakub Strzemieczny, Boguslawa Musial, Agnieszka Pawlaczyk, Malgorzata Sznitowska, Danuta Siluk
Summary: With the increase in environmental pollution and smoking, the number of patients with chronic obstructive pulmonary disease is increasing, leading to a growing demand for generic products for its treatment. This study presents the research on a newly patented single-dose inhaler, which shows that using an inhaler with specific fluid dynamics features can increase the dose of glycopyrronium bromide inhalation.
ACTA POLONIAE PHARMACEUTICA
(2022)
Article
Biochemistry & Molecular Biology
Wiktoria Struck-Lewicka, Beata Karpinska, Wojciech Rodzaj, Antoni Nasal, Bartosz Wielgomas, Michal Jan Markuszewski, Danuta Siluk
Summary: In this study, a thin film solid phase microextraction method (TF-SPME) was developed and optimized for metabolomics profiling of eight steroid compounds from urine samples. The most crucial steps in the process were found to be sample extraction and analyte desorption. The proposed TF-SPME method, coupled with UHPLC-ESI-QTOF/MS, showed acceptable validation parameters and was successfully used for the isolation of steroid hormones from urine samples.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marta Kordalewska, Renata Wawrzyniak, Julia Jacyna, Joanna Godzien, Angeles Lopez Gonzalves, Joanna Raczak-Gutknecht, Marcin Markuszewski, Piotr Gutknecht, Marcin Matuszewski, Janusz Siebert, Coral Barbas, Michal J. Markuszewski
Summary: This study applied a multiplatform untargeted metabolomics analysis of urine to search for a metabolic pattern specific for renal cell carcinoma (RCC) and clear cell renal cell carcinoma (ccRCC). The analysis revealed disruptions in amino acid, lipid, purine, and pyrimidine metabolism, as well as the TCA cycle and energetic processes. Interestingly, the study also identified changes in the levels of modified nucleosides, which had not been previously observed in RCC and ccRCC patients. Additionally, the study found statistically significant changes in symmetric dimethylarginine (SDMA) in RCC using the CE-MS technique.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Veterinary Sciences
Vincent Marolf, Keila K. Ida, Danuta Siluk, Wiktoria Struck-Lewicka, Michal J. Markuszewski, Charlotte Sandersen
Summary: The study showed that combining ropivacaine with dexmedetomidine could prolong sensory nerve blockade in dogs while minimizing adverse effects.
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Katarzyna Polonis, Renata Wawrzyniak, Emilia Daghir-Wojtkowiak, Anna Szyndler, Marzena Chrostowska, Olle Melander, Michal Hoffmann, Marta Kordalewska, Joanna Raczak-Gutknecht, Ewa Bartosinska, Roman Kaliszan, Krzysztof Narkiewicz, Michal J. Markuszewski
FRONTIERS IN MOLECULAR BIOSCIENCES
(2020)