Journal
CHROMATOGRAPHIA
Volume 78, Issue 3-4, Pages 157-162Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-014-2821-7
Keywords
Thermoresponsive; Boronate affinity; cis-Diol; Poly(N-isopropylacrylamide) (PNIPAAm); 4-Vinylphenylboronic acid (VPBA)
Funding
- National Nature Science Foundation of China [21275019]
- National Key Technology R&D Program of China [2009BAK59B01]
- National Key Scientific Instrument and Equipment Development Projects of China [2012YQ040140]
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Based on the successful graft of P(N-isopropylacrylamide-4-vinylphenylboronic acid) [P(NIPAAm-co-VPBA)] on glass substrates by surface-initiated atom transfer radical polymerization, a microfludic chip with the performance of thermoresponsive boronate affinity was fabricated and was used for capture-release of the cis-diol biomolecule adenosine. The copolymers modified successfully on the surface of glass substrate were characterized by Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results demonstrated that, by simply adjusting the temperature from 4 to 55 A degrees C, the microchip grafted with P(NIPAAm-co-VPBA) was specific to the cis-diol-containing molecules, and can selectively and quickly realize the capture and release of the cis-diol biomolecule adenosine from the mixture of adenosine and deoxyadenosine, which was validated by high performance liquid chromatography/electronic spray ion-ion trap mass spectrometry (HPLC/ESI-ion trap MS). Moreover, less than 10 min was taken from injection to the collection of the sample at the end of the channel. With further development, the P(NIPAAm-co-VPBA)-grafted microchip with high selectivity and repeatability may be chosen as a promising tool for the enrichment of the cis-diol-containing biomolecules, such as glycoproteins, carbohydrates, glycosylated peptides, nucleosides, etc.
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