Article
Biochemical Research Methods
Shengnan Ji, Xiaomeng Liu, Jing Ha, Lianfeng Ai, Zheng Li
Summary: A sensitive and simple LC-MS/MS method was developed for the determination of orelabrutinib in human plasma and cerebrospinal fluid. The method involved simple protein precipitation, efficient separations, and optimized mass spectrometric conditions to increase selectivity and sensitivity. The method was validated for accuracy, precision, selectivity, linearity, recovery, matrix effect, stability, and limits of quantification. It exhibited a lower limit of quantification of 0.50 ng/mL, with intraday and interday precision RSD both less than 15%, and a recovery rate of 85.7%-92.9%. The method was successfully applied for quantitation in clinical patients treated with orelabrutinib.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Biochemistry & Molecular Biology
Nicolas Sambiagio, Jean-Jacques Sauvain, Aurelie Berthet, Reto Auer, Anna Schoeni, Nancy B. Hopf
Summary: Human biomonitoring of oxidative stress often relies on urinary biomarkers such as 8-oxodG and 8-isoprostane, but inconsistent levels reported in scientific literature can be attributed to the various analytical methods used. A newly developed UPLC-MS/MS method allows for simultaneous quantification of both biomarkers with high accuracy and precision. This method shows promise for biomonitoring and epidemiological studies focusing on oxidative damage.
Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Abdullah M. M. Faqehi, Scott G. Denham, Gregorio Naredo, Diego F. Cobice, Shazia Khan, Joanna P. Simpson, Ghazali Sabil, Rita Upreti, Fraser Gibb, Natalie Z. M. Homer, Ruth Andrew
Summary: Liquid Chromatography tandem mass spectrometry (LC-MS/MS) is the preferred method for androgen analysis in biological fluids due to its high selectivity. Using derivatization approaches with hydrazine-based reagents, particularly 2-hydrazino-1-methylpyridine (HMP), enhances sensitivity for analysis of low plasma volumes. Chromatographic separation and careful analysis show that HMP derivatives offer higher sensitivity compared to HTP derivatives.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Dun-Jian Wang, Da-Wei Wang, Qiu-Chen Fang, Ye Shen, Nv-Jin Zeng, Yan-Ling Yang, Hong-Wen Zhang, Yong-Qing Wang, Lu-Ning Sun
Summary: A simple, fast, and sensitive tandem mass spectrometry method was established to detect the concentration of regadenoson in human plasma, which was used to study the pharmacokinetic characteristics of regadenoson in healthy Chinese subjects. The method showed linear calibration curve, low precision, high accuracy, and no matrix effect for regadenoson or regadenoson-d3.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Masaki Takiwaki, Fumio Nomura, Mamoru Satoh, Sachio Tsuchida, Kazuo Otake, Junko Takagi
Summary: This study aimed to develop an LC/MS/MS-based method for detecting human plasma AVP and compare it with the traditional RIA method. Although there was a weak overall correlation between the results obtained using the two different methods, disagreements were observed at low concentrations.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Applied
Xuan Zhang, Changling Fang, Dongmei Huang, Guangxin Yang, Yunyu Tang, Yongfu Shi, Cong Kong, Pei Cao, Youqiong Cai
Summary: A method for the determination of 8 biogenic amines in aquatic products and their derived products was established by HPLC-MS/MS without derivatization. The method achieved good linearity, higher sensitivity, and demonstrated average recoveries in the range of 70.9% to 113.1% with low relative standard deviations. It was proven suitable for the detection of BAs in aquatic products and their products.
Article
Medical Laboratory Technology
Jenny Yeuk-Ki Cheng, Felix Chi-Kin Wong, Edith Wing-Kar Chow, Wendy Wan-Hang Lau, Kitty Kit-Ting Cheung, Timothy Hua-Tse Cheng, Teresa Kam-Chi Tsui, Alan Shek-Lun Chan, Clara Wai-Shan Lo, Chung-Shun Ho
Summary: This study provides age-specific LC-MS/MS reference intervals for PRA and PAC in both normotensive and EH populations for local Chinese in Hong Kong.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Chemistry, Analytical
Yongjuan Jia, Xingli Liu, Li Xu, Jinbao Zhao, Junjun Ni, Yuanyuan Zhang
Summary: A high-throughput solid-phase extraction method was developed for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography-tandem mass spectrometry in this study. The method showed good linearities and sensitivity for each steroid hormone within the quantitative range. It has been successfully applied to series of samples from clinical trials with no significant difference between serum and ethylenediaminetetraacetic acid plasma samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mateus Araujo Castro e Souza, Naialy Fernandes Araujo Reis, Isabella Cristina Placido de Oliveira Pacheco, Maria Auxiliadora Parreiras Martins, Maria Beatriz A. Gloria, Gerson Antonio Pianetti, Christian Fernandes
Summary: This study developed a modern method for the simultaneous determination of eight cardiovascular drugs in human plasma. The method is simple, fast, environmentally friendly, and has high accuracy and reliability.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Natalia G. P. Santos, Deyber A. V. Medina, Fernando M. Lancas
Summary: This study explores the application of WCOT column in LC-MS/MS. The column preparation process encompasses column pretreatment, silanization, stationary phase coating, and immobilization. Various parameters that may affect column efficiency, including stationary phase composition, length, internal diameter, mass, and injection volume, are evaluated. The optimized WCOT column, with a length of 8 m, an inner diameter of 25 μm, OV-210 mass of 2.5% m/v, and injection volume of 100 nL, coupled with a commercial trapping column in the nanoLC system, demonstrates improved separation resolution.
Article
Pharmacology & Pharmacy
Fen Jiang, Jong-Yeol Choi, Ju-Hyun Lee, Sunae Ryu, Ze-Won Park, Jong-Gu Lee, Han-Sung Na, Seok-Yong Lee, Woo-Yong Oh, Myeon-Woo Chung, Seung-Eun Choi
Article
Oncology
Yao-Bin Zhu, Jie-Wei Luo, Fen Jiang, Gui Liu
MOLECULAR MEDICINE REPORTS
(2018)
Article
Oncology
Shuiqing Wu, Fen Jiang, Hongtao Wu, Yinhuai Wang, Ran Xu, Jian Cao, Qiong Lu, Xuan Zhu, Zhaohui Zhong, Xiaokun Zhao
MOLECULAR MEDICINE REPORTS
(2018)
Letter
Pharmacology & Pharmacy
Fen Jiang, Jae-Gook Shin
JOURNAL OF CLINICAL PHARMACOLOGY
(2013)
Article
Pharmacology & Pharmacy
A. M. Abdelhady, Z. Desta, F. Jiang, C. W. Yeo, J. G. Shin, B. R. Overholser
JOURNAL OF CLINICAL PHARMACOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Fen Jiang, Chang-Woo Yeo, Sang-Seop Lee, Min-Kyung Oh, Jong-Lyul Ghim, Ji-Hong Shon, Ho-Sook Kim, Eun-Young Kim, Dong-Hyun Kim, Jae-Gook Shin
PHARMACOGENETICS AND GENOMICS
(2013)
Article
Neurosciences
Fen Jiang, Hae-Deun Kim, Han-Sung Na, Seok-Yong Lee, Doo-Won Seo, Jong-Yeol Choi, Ji-Hye Ha, Hee-Jung Shin, Young-Hoon Kim, Myeon-Woo Chung
PSYCHOPHARMACOLOGY
(2015)
Article
Chemistry, Medicinal
Euna Cho, Eun Jin Ryu, Fen Jiang, Ung Bae Jeon, Mong Chou, Cy Hyun Kim, Miyoung Kim, Nam Deuk Kims, Tae-Ho Hwang
DRUG DESIGN DEVELOPMENT AND THERAPY
(2018)
Article
Oncology
Euna Cho, S. M. Bakhtiar Ul Islam, Fen Jiang, Ju-Eun Park, Bora Lee, Nam Deuk Kim, Tae-Ho Hwang
CANCER RESEARCH AND TREATMENT
(2020)
Article
Oncology
S. M. Bakhtiar Ul Islam, Bora Lee, Fen Jiang, Eung-Kyun Kim, Soon Cheol Ahn, Tae-Ho Hwang
Article
Oncology
Jeong-Oh Kim, Jung-Young Shin, Seo Ree Kim, Kab Soo Shin, Joori Kim, Min-Young Kim, Mi-Ran Lee, Yonggoo Kim, Myungshin Kim, Sook Hee Hong, Jin Hyoung Kang
Article
Oncology
Jin-Hyoung Kang, Ki-Hyeong Lee, Dong-Wan Kim, Sang-We Kim, Hye Ryun Kim, Joo-Hang Kim, Jin-Hyuk Choi, Ho Jung An, Jin-Soo Kim, Joung-Soon Jang, Bong-Seog Kim, Heung Tae Kim
Summary: Belotecan shows potential advantages over topotecan in terms of efficacy and safety for sensitive-relapsed SCLC, especially in younger age, more advanced disease, or poor performance patients. Further evaluation in Phase 3 trials is warranted.
BRITISH JOURNAL OF CANCER
(2021)
Article
Oncology
Jung-Young Shin, Jeong-Oh Kim, Mi-Ran Lee, Seo Ree Kim, Kyongmin Sarah Beck, Jin Hyoung Kang
Article
Medicine, General & Internal
Joon-Mo Kim, Kyung-Rim Sung, Hwang-Ki Kim, Sang-Woo Park, Eun-Ji Lee, Jin-Wook Jeoung, Hae-Young Lopilly Park, Jaehong Ahn, Chungkwon Yoo, Chan-Yun Kim
Summary: This multicenter retrospective cohort study compared the effectiveness and safety of long-term tafluprost, travoprost, or latanoprost in patients with primary open-angle glaucoma (POAG) or normal-tension glaucoma (NTG). The study found that all three prostaglandin analogs showed similar effects in patients with POAG or NTG, with good long-term safety profiles.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Yeon Jeong Kim, Fen Jiang, Jin Park, Hyeon Hee Jeong, Ji Eun Baek, Seung-Mo Hong, Seong-Yun Jeong, Sang Seok Koh
Summary: The study reveals the tumor-promoting role of PAUF in ovarian cancer and suggests that an anti-PAUF antibody may have therapeutic effects on high PAUF-expressing ovarian cancer.
FRONTIERS IN PHARMACOLOGY
(2022)