Journal
CHROMATOGRAPHIA
Volume 75, Issue 3-4, Pages 175-180Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-011-2163-7
Keywords
High-performance liquid chromatography; Tandem mass spectrometry; Milk thistle; Silymarin; Silybin
Funding
- Hungary-Romania Cross-Border Co-operation Programme [HURO/0901/058/2.2.2]
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A high-performance liquid chromatographic/tandem mass spectrometric method was developed for the determination of the major bioactive flavonolignans in silymarin, a herbal remedy extracted from the milk thistle Silybum marianum. In this study, eight active components of silymarin with the same elemental composition, including silychristins A and B, silydianin, silybin A and B, isosilybin A and B and an unknown compound were completely separated. Furthermore, three additional components were detected and partly separated; presumably two silybin stereoisomers and one isosilybin stereoisomer. The collision-induced dissociation (CID) MS/MS spectra of these silymarin constituents were studied: the spectral similarity values of the component pairs were determined, and simple criteria were found for distinguishing the components.
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