Journal
CHROMATOGRAPHIA
Volume 68, Issue 5-6, Pages 381-386Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-008-0727-y
Keywords
immobilized metal affinity chromatography; phosphorylation; metal ions
Funding
- Ministry of Education of the Czech Republic [MSM 0021620806, LC 06044]
- Institute of Pathophysiology
- Center of Experimental Hematology at Charles University in Prague
Ask authors/readers for more resources
The interactions of two model phosphoproteins (porcine pepsin and ovalbumin) with two different immobilized metal affinity chromatography (IMAC) sorbents containing immobilized Fe(3+), Ga(3+), and UO(2)(2+) ions have been investigated under various conditions. Both proteins were adsorbed on immobilized uranyl ions under acidic conditions similar to those on immobilized Fe(3+) and Ga(3+) ions. The retained proteins could be released either by the presence of phosphate ions in the elution buffer (immobilized Ga(3+) and Fe(3+) ions) or by an increased pH (all tested immobilized ions). The IMAC sorbents employed could be used under the conditions of high-performance chromatography and are suitable for the separation and analysis of intact phosphoproteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available