Article
Biochemistry & Molecular Biology
Suman Yadav, Anand Kumar Pandey, Suresh Kumar Dubey
Summary: The study identified a novel strain with highly efficient thermostable, glucose tolerant beta-glucosidases. Molecular docking simulations revealed the enzyme's ability to effectively hydrolyze beta-1-4 linkages with specific contributing amino acids. Additionally, a distinct glucose-binding site was found, suggesting limited competitive inhibition by glucose, making this enzyme a promising candidate for cellulose degradation and various industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agronomy
Yining Huang, Liguang Gao, Ming Lin, Ting Yu
Summary: The modified safety-secured yeast showed great potential in overexpressing antimicrobial peptides, improving biocontrol ability. The recombinant strains demonstrated significant suppression of pathogens and could be promising biological agents in postharvest applications.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yi He, Chenxi Wang, Ronghu Jiao, Qinxue Ni, Yan Wang, Qianxin Gao, Youzuo Zhang, Guangzhi Xu
Summary: In this study, a glucose-tolerant beta-glucosidase gene (Bgl1973) was cloned from Leifsonia sp. ZF2019 and expressed heterologously in E. coli. The enzyme showed excellent salt tolerance and glucose tolerance with high specific activity. Molecular docking analysis revealed the characteristics of its glucose binding location and active channel geometry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hai-Yan Zhou, Qi Chen, Yi-Feng Zhang, Dou-Dou Chen, Xiao-Nan Yi, De-Shui Chen, Xin-Ping Cheng, Mian Li, Hong-Yan Wang, Kai-Qian Chen, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: Structural modification of Coniophora puteana beta-glucosidase (CpBgl) resulted in increased enzyme activity of mutants CpBgl-Q20C and CpBgl-A240S by 65.75% and 58.58%, respectively. These mutants exhibited improved stability and enhanced activity in the presence of Mn2+ or Fe2+. The Vmax and catalysis efficiency of the mutants were significantly improved compared to the wild-type CpBgl. Practical degradation experiments using CpBgl-Q20C showed increased glucose yields in different biomasses. The findings contribute to the understanding of enzyme-substrate binding and have implications for industrial bio-degradation of cellulosic biomass.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Qi Shen, Zhuang Yu, Xiao-Ting Zhou, Shi-Jia Zhang, Shu-Ping Zou, Neng Xiong, Ya-Ping Xue, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: A novel glucose-regulated promoter, P-Dog2p300, was identified for driving the expression of antibiotic-resistant genes in Pichia pastoris, resulting in reduced metabolic burden during recombinant protein expression. The use of P-Dog2p300 effectively selected transformants harboring multiple integrations in a cost-effective manner.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ryota Kumokita, Takanobu Yoshida, Tomokazu Shirai, Akihiko Kondo, Tomohisa Hasunuma
Summary: Aromatic secondary metabolites are widely used in various industries and their production currently relies on plant extraction. Microbe-based processes have recently attracted attention as sustainable alternatives. This study found that the yeast Pichia pastoris can produce higher levels of aromatic secondary metabolites from glycerol compared to glucose, and enhanced utilization of specific amino acids further increases the production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
David Alejandro Gutierrez-Gutierrez, Jose Antonio Fuentes-Garibay, Jose Maria Viader-Salvado, Martha Guerrero-Olazaran
Summary: In this study, we engineered Pichia pastoris strains to produce recombinant 8-glucosidase Glu1B from the termite Coptotermes formosanus. The recombinant enzyme showed high catalytic activity and resistance to glucose inhibition, making it a suitable supplement for cellulose hydrolysis in lignocellulosic biomass saccharification.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tianpeng Chen, Shimeng Wang, Huanqing Niu, Guanjia Yang, Sinan Wang, Yuqi Wang, Chaowei Zhou, Bin Yu, Pengpeng Yang, Wenjun Sun, Dong Liu, Hanjie Ying, Yong Chen
Summary: A biofilm-based fermentation system was constructed in P. pastoris by combining the surface display of beta-galactosidase and biofilm formation. The results showed a catalytic conversion rate of 50.3% for GOS with a maximum enzyme activity of 5125 U/g. This study provides a basis for developing biofilm-based surface display methods in P. pastoris.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
Minjie Gao, Yuchen Zhou, Jiajun Yan, Li Zhu, Zhitao Li, Xiuyu Hu, Xiaobei Zhan
Summary: Rhodium is widely used in catalysis, but its adsorption by microorganisms is not well studied. In this work, waste P. pastoris and recombinant P. pastoris GS-R were used as novel adsorbents for Rh(III) adsorption, showing high adsorption capacity and selectivity. The study developed an environmentally-friendly strategy for recovering Rh(III) and other precious metals using P. pastoris.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Weiwang Dou, Quanchao Zhu, Meihua Zhang, Zuyuan Jia, Wenjun Guan
Summary: This study identified 8 strong endogenous promoters in P. pastoris using RNA-seq and LacZ reporter system, which contributed to higher transcriptional expression levels and beta-galactosidase activities. These promoters were applied to a heterologous beta-carotene biosynthetic pathway, resulting in the highest yield of beta-carotene up to 1.07 mg/g. Simultaneously using the same promoter multiple times may lead to a notable competitive effect and lower the transcriptional expression level of the target gene.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Yan-xiao Li, Nan-nan Wang, Qiao-juan Yan, Xiao-han Hua, Yu Liu, Zheng-qiang Jiang
Summary: This study successfully expressed a novel beta-mannanase gene for the production of partially hydrolyzed konjac powder (PHKP). The enzyme showed unique hydrolysis properties and was used as a fiber supplement for steamed bread preparation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Anmoldeep Randhawa, Nandita Pasari, Tulika Sinha, Mayank Gupta, Anju M. Nair, Olusola A. Ogunyewo, Sandhya Verma, Praveen Kumar Verma, Syed Shams Yazdani
Summary: In this study, we addressed the issue of drug tolerance in Penicillium funiculosum and developed an optimized toolkit for genome modification. We laid the foundation for gene function analysis and further genetic improvements of P. funiculosum using both traditional and advanced methods.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Environmental Sciences
Yu-Ying Huang, Pei Wu, Xing-Ci Wu, Qian-Ru Zhu, Qian Zhu, Hong-Zhao Zheng, Dan Zhu, Zhi-Hua Lv, Yi-Rui Yin
Summary: In this study, a new thermophilic and inhibitor-tolerant beta-glucosidase gene, ThBg2, was obtained from a metagenome of Ruidian Hot Spring. The gene was synthesized, cloned, heterologously expressed, and enzymatically characterized. ThBg2 showed high activity and stability within a certain temperature and pH range.
Article
Biochemistry & Molecular Biology
In Jung Kim, Uwe T. Bornscheuer, Ki Hyun Nam
Summary: In this study, the biochemical and structural analysis of a Bgl enzyme (TsaBgl) from the hemicellulose-degrading bacterium Thermoanaerobacterium saccharolyticum was reported. TsaBgl exhibited maximum hydrolase activity at pH 6.0 and 55°C and showed a unique structure compared to other known Bgls. The study improved our understanding of the inhibition and glucose tolerance mechanisms of Bgl enzymes, which is crucial for their industrial application.
Article
Microbiology
Jose Juan Mateo
Summary: A variety of useful microorganisms, including Metschnikowia pulcherrima, live on the surface of grapes. This particular yeast secretes a beta-glucosidase that can release aromatic compounds during fermentation processes. The synthesis of an exocellular beta-glucosidase was demonstrated in this study, and the optimal conditions to maximize its effectiveness were determined. The enzyme showed high activity at 28 degrees C and pH 4.5, as well as tolerance to glucose, fructose, ethanol, and methanol. Furthermore, its activity was enhanced by calcium ions and low concentrations of ethanol and methanol. The impact of terpene content in wine was also investigated. Due to these characteristics, beta-glucosidase is a promising candidate for use in enology.
Article
Materials Science, Biomaterials
Raman Dhivya, Jothi Ranjani, Jeyaprakash Rajendhran, Jeyanthinath Mayandi, Jamespandi Annaraj
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2018)
Article
Microbiology
Sathyanarayanan Jayashree, Raman Karthikeyan, Sampath Nithyalakshmi, Jothi Ranjani, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Swati Sahay, Rajeswari Shome, Jagadesan Sankarasubramanian, Udayakumar S. Vishnu, Awadhesh Prajapati, Krithiga Natesan, Bibek Ranjan Shome, Habibur Rahman, Jeyaprakash Rajendhran
ANNALS OF MICROBIOLOGY
(2019)
Article
Chemistry, Applied
Raman Dhivya, Sellamuthu Kathiresan, Murugesan Vigneshwar, Jothi Ranjani, Jeyaprakash Rajendhran, Nattamai S. P. Bhuvanesh, Jamespandi Annaraj
APPLIED ORGANOMETALLIC CHEMISTRY
(2019)
Article
Infectious Diseases
Jagadesan Sankarasubramanian, Udayakumar S. Vishnu, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
INFECTION GENETICS AND EVOLUTION
(2019)
Article
Green & Sustainable Science & Technology
S. Santha Kalaikumari, T. Vennila, V. Monika, K. Chandraraj, P. Gunasekaran, J. Rajendhran
JOURNAL OF CLEANER PRODUCTION
(2019)
Article
Microbiology
Raman Karthikeyan, Pratapa Gayathri, Paramasamy Gunasekaran, Medicharla Jagannadham, Jeyaprakash Rajendhran
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Genetics & Heredity
Ramamoorthy Sivakumar, Jothi Ranjani, Udayakumar S. Vishnu, Sathyanarayanan Jayashree, Gabriel L. Lozano, Jessica Miles, Nichole A. Broderick, Changhui Guan, Paramasamy Gunasekaran, Jo Handelsman, Jeyaprakash Rajendhran
G3-GENES GENOMES GENETICS
(2019)
Article
Biochemistry & Molecular Biology
Ramamoorthy Sivakumar, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
MOLECULAR BIOLOGY REPORTS
(2020)
Article
Microbiology
Parameswaran Sree Pranav, Balasubramanian Mahalakshmi, Ramamoorthy Sivakumar, Raman Karthikeyan, Jeyaprakash Rajendhran
Summary: The study isolated a strain of Paenibacillus from soil with antimicrobial activity against various drug-resistant pathogens. Genome mining and similarity network construction identified multiple secondary metabolites BGCs, some of which showed high similarity to known antibiotics while others were unique or had low similarity. Further experimental investigations could lead to the discovery of novel antimicrobial drugs.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Manjula Arumugam, Sathyavathi Sundararaju, Sankarasubramanian Jagadesan, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Summary: The study revealed that the microbial community in the termite nest is dominated by Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes, with a high diversity of glycosyl hydrolase genes. Particularly, Actinobacteria is the main contributor of cellulolytic and hemicellulolytic enzymes. The termite nest has a unique microbial community that efficiently degrades lignocelluloses, with differences in functional profiles compared to other lignocellulolytic communities.
CURRENT MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ramamoorthy Sivakumar, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Summary: Sigma factors play a crucial role in bacteria by modulating the plasticity of RNA polymerase and promoter recognition. In this study, the regulatory role of AlgU sigma factor in Pseudomonas aeruginosa PGPR2 during root colonization was investigated. The inactivation of the AlgU gene led to decreased root colonization efficiency, increased sensitivity to osmotic and antibiotic stresses, altered biofilm formation, and changed motility. These findings highlight the importance of AlgU sigma factor in modulating gene expression during root colonization.
MOLECULAR GENETICS AND GENOMICS
(2022)
Article
Biotechnology & Applied Microbiology
Ramamoorthy Sivakumar, Parameswaran Sree Pranav, Madhavi Annamanedi, S. Chandrapriya, Shrikrishna Isloor, Jeyaprakash Rajendhran, Nagendra R. R. Hegde
Summary: This study conducted a comprehensive analysis of bovine mastitis-associated S. aureus isolates from India using whole genome sequencing and comparative genomic analysis. The results revealed 15 different sequence types and 5 clonal complexes. The genome of Indian mastitis-associated S. aureus strains is relatively closed. Phylogenetic analysis based on SNP variation differentiated the 41 strains into six major clades. Seventeen different antimicrobial resistance genes and 108 virulence-associated genes were identified among these isolates.
Article
Microbiology
Raman Karthikeyan, Pratapa Gayathri, Subbiah Ramasamy, Vemparthan Suvekbala, Medicharla Jagannadham, Jeyaprakash Rajendhran
Summary: Membrane vesicles (MVs) are important virulence factors in pathogenic bacteria. The role of MVs in the pathogenesis of Listeria monocytogenes is still not fully understood. In this study, we found that exposure to MVs up-regulated autophagy-related genes and induced the transcription of inflammatory cytokines in Caco-2 cells. Pathway analysis confirmed that MVs primarily induce immune signaling pathways and promote the survival of L. monocytogenes by prolonging host cell survival and manipulating gene expression. We also identified non-coding RNAs (ncRNAs) that may play a crucial role in the early manipulation of host gene expression.
CURRENT RESEARCH IN MICROBIAL SCIENCES
(2023)
Article
Microbiology
Vishal Singh Somvanshi, Bhumika Dubay, Jyoti Kushwah, Sivakumar Ramamoorthy, Udayakumar S. Vishnu, Jagadesan Sankarasubramanian, Jeyaprakash Rajendhran, Uma Rao
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)