Article
Medicine, Research & Experimental
P. Vishnyakova, A. Poltavets, E. Karpulevich, A. Maznina, V. Vtorushina, L. Mikhaleva, E. Kananykhina, A. Lokhonina, S. Kovalchuk, A. Makarov, A. Elchaninov, G. Sukhikh, T. Fatkhudinov
Summary: Macrophages play a crucial role in innate immunity and are activated by various factors. This study found that macrophages derived from CD14+ monocytes exhibit higher sensitivity to pro-inflammatory induction, showing increased levels of pro-inflammatory and anti-inflammatory cytokines compared to CD16+-derived macrophages. This data may be valuable for macrophage-based cell therapy applications, providing insights for manipulating donor monocytes for transplantation.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Immunology
Matheus B. Carneiro, Nathan C. Peters
Summary: This review focuses on the interaction between Leishmania amazonensis infection and host innate immune response, particularly addressing the impact of adaptive immunity on phagocytic host cell recruitment and activation. Experimental observations indicate that most mouse strains are susceptible to L. amazonensis infection, unlike other Leishmania species. The CD4(+) T helper (Th)1/Th2 paradigm does not fully explain the chronic disease established by L. amazonensis, with recent findings complicating the understanding of the Th1/Th2 paradigm.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Anne-Marije Hulshof, H. Coenraad Hemker, Henri M. H. Spronk, Yvonne M. C. Henskens, Hugo ten Cate
Summary: Fibrinogen is not only involved in clot formation, but also removes thrombin from solution and activates other clotting factors, as well as regulating the balance between the immune system and the coagulation system through activating immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Florian Rosier, Nicolas Fernandez Nunez, Magali Torres, Beatrice Loriod, Pascal Rihet, Lydie C. Pradel
Summary: In this study, the transcriptional response of mice injected with LPS was compared to human cells stimulated with LPS in vitro and the blood cells of septic patients. The results showed a significant overlap in gene regulation and disease processes between mouse and human sepsis, supporting the hypothesis of common molecular and cellular mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Ann-Charlotte Stromdahl, Lech Ignatowicz, Ganna Petruk, Marta Butrym, Sebastian Wasserstrom, Artur Schmidtchen, Manoj Puthia
Summary: The study demonstrates that TCP-25 delivered from PU exhibits a combined antibacterial and anti-inflammatory effect both in vitro and in vivo, showing significant efficacy in treating wound infections and inflammation.
ACTA BIOMATERIALIA
(2021)
Article
Critical Care Medicine
Julia Leonhardt, Mirrin J. Dorresteijn, Sophie Neugebauer, Diana Mihaylov, Julia Kunze, Ignacio Rubio, Frank-Stephan Hohberger, Silke Leonhardt, Michael Kiehntopf, Klaus Stahl, Christian Bode, Sascha David, Frank A. D. T. G. Wagener, Peter Pickkers, Michael Bauer
Summary: The study found that circulating bile acids capable of inducing immunosuppression are present in septic shock patients, particularly in those with severe liver failure. Future research may need to explore whether modulating bile acid metabolism can improve the clinical course and outcome of sepsis in these patients.
Article
Cardiac & Cardiovascular Systems
Sabrina Geisberger, Hendrik Bartolomaeus, Patrick Neubert, Ralf Willebrand, Christin Zasada, Thomas Bartolomaeus, Victoria McParland, Dries Swinnen, Anneleen Geuzens, Andras Maifeld, Luka Krampert, Marion Vogl, Anja Mahler, Nicola Wilck, Lajos Marko, Ekin Tilic, Sofia K. Forslund, Katrina J. Binger, Johannes Stegbauer, Ralf Dechend, Markus Kleinewietfeld, Jonathan Jantsch, Stefan Kempa, Dominik N. Mueller
Summary: Research shows that high salt intake leads to intracellular sodium accumulation, inhibiting mitochondrial respiration in macrophages and affecting immune cell function. While these changes may help resolve bacterial infections, they could also accelerate the development of inflammatory cardiovascular diseases.
Review
Plant Sciences
Miwa Shirai, Thomas Eulgem
Summary: Macrophomina phaseolina, a highly diverse and plastic fungal pathogen, poses challenges in plant disease management. Although some studies have reported host defense measures and pathogenic mechanisms, no robust defense mechanism against M. phaseolina has been identified.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Jintao Feng, Wei Jiang, Xiaofang Cheng, Benkun Zou, Alan W. Varley, Ting Liu, Guojun Qian, Wenjiao Zeng, Jianguo Tang, Qiang Zhao, Yiwei Chu, Yuanyuan Wei, Xiaobo Li, Robert S. Munford, Mingfang Lu
Summary: Research showed that acyloxyacyl hydrolase (AOAH) can prevent foam cell formation in mice, reduce cholesterol accumulation, and inflammation. Aoah(-/-) mice developed more arterial foam cells after being fed a high fat, high cholesterol diet and receiving small doses of LPSs.
Article
Cell Biology
Xue-Kun Guo, Jiali Wang, Vincent P. van Hensbergen, Jintao Liu, Huji Xu, Xiaoyu Hu
Summary: This study reveals a unique pattern of crypt occupation by mucus-associated biofilms during early colitis, which is genetically dependent on bacterial biofilm-forming capacity and restricted by host epithelial O 1,2-fucosylation. Deficiency in O 1,2-fucosylation leads to increased crypt occupation by biofilms and exacerbated intestinal inflammation. Mechanistically, fucose from biofilm-occupied mucus suppresses biofilm formation and related genes. Additionally, fucose administration ameliorates experimental colitis, suggesting the therapeutic potential of fucose for biofilm-related disorders.
Article
Biochemistry & Molecular Biology
Frederic V. Schwabe, Lotta Happonen, Sofie Ekestubbe, Ariane Neumann
Summary: This study investigated the effects of LL-37 and LTF on blood-derived CD14(+) monocytes and found that these HDPs can induce the release of TNF alpha, nucleosomes, and monocyte ETs. The formation of ETs by LL-37 is dependent on SOCE, MAPK, and ERK1/2, while the release of ETs by LTF is not affected by any inhibitors used in this study.
Article
Medicine, Research & Experimental
Hassan Ahmed, Urooj Amin, Xiaolun Sun, Demetrius R. Pitts, Yunbo Li, Hong Zhu, Zhenquan Jia
Summary: Lipopolysaccharide (LPS) is an endotoxin that can cause severe inflammation and septic shock. This study shows that the triterpenoid compound CDDO-IM can reduce LPS-induced inflammation in macrophage cells and protect them from cytotoxicity by upregulating antioxidant molecules.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Yanqin Li, Xuan Li, Xu Chen, Xiaoqian Sun, Xiangning Liu, Gang Wang, Yizhou Liu, Lingwen Cui, Tianhua Liu, Wei Wang, Yong Wang, Chun Li
Summary: Qishen granule has been proven to be effective in treating heart failure in China by regulating splenic monocytes release and myocardial inflammatory response through the TLR4-MyD88-NF-κB pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Chemistry, Medicinal
Tamara Fedorovna Solov'eva, Svetlana Ivanovna Bakholdina, Gennadii Alexandrovich Naberezhnykh
Summary: This article summarizes the potential of LPS-binding proteins from marine invertebrates as drugs for sepsis treatment. The structure, properties, and activity of these proteins, as well as the potential problems in clinical trials are discussed in detail.
Article
Chemistry, Multidisciplinary
Zhongqian Qiao, Wenjing Zhang, Yueming Wu, Weinan Jiang, Ning Shao, Jiayang Xie, Guixue Xia, Qimeng Chen, Zhefeng Liu, Jingcheng Zou, Jiawei Gu, Shifang Luan, Haodong Lin, Runhui Liu
Summary: Wound infections are a significant health issue caused by the invasion and spread of pathogens in wound sites. However, antibiotic resistance, particularly in MRSA, has made it challenging to treat these infections. To address MRSA-infected wounds, an antibacterial peptide polymer-loaded hyaluronic acid (HA) hydrogel (Gel-HA@P) has been developed. This design mimics host defense peptides, exhibiting potent antibacterial activity, low susceptibility to drug resistance, and good stability. Gel-HA@P demonstrates effective activity against MRSA, low toxicity, and promotes wound healing. This strategy provides an antibiotic-free approach for the healing of MRSA-infected wounds.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Biochemical Research Methods
Humaira Ilyas, Mariena J. A. van der Plas, Monica Agnoletti, Sourav Kumar, Atin Kumar Mandal, Hanudatta S. Atreya, Anirban Bhunia, Martin Malmsten
Summary: The study investigated how PEGylation affects the interaction of host defense peptides with bacterial lipopolysaccharide and the suppression of LPS-induced cell activation. The results showed that PEGylation did not compromise the anti-inflammatory effects of the peptides and even improved the suppression of LPS-induced cell activation.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Biology
Mariena J. A. van der Plas, Jun Cai, Jitka Petrlova, Karim Saleh, Sven Kjellstrom, Artur Schmidtchen
Summary: The study characterized the peptidome of human wound fluids using mass-spectrometry analyses for the first time, revealing a higher degree of peptides in sterile post-surgical wound fluids compared to human plasma. The unique peptide patterns identified in uninfected healing wounds and Staphylococcus aureus-infected wounds suggest the potential of using these peptides as biomarkers for wound healing and infection.
Article
Chemistry, Physical
Elisa Parra-Ortiz, Martin Malmsten
Summary: This overview focuses on the photocatalytic antimicrobial effects of nanomaterials, which generate reactive oxygen species upon light exposure to cause oxidative damage to key components in bacteria and viruses. Understanding the mechanistic foundation behind these effects is still in its early stages.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Alma Martin, Jun Cai, Anna-Lena Schaedel, Mariena van der Plas, Martin Malmsten, Thomas Rades, Andrea Heinz
Summary: This study aims to develop fibers with enhanced mechanical strength and water stability, as well as sustained release of tetracycline hydrochloride. By using zein as core material and polycaprolactone as shell material, the researchers achieved improved cell attachment and wound closure, while maintaining continuous drug release.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Alma Martin, Jenny Natalie Nyman, Rikke Reinholdt, Jun Cai, Anna-Lena Schaedel, Mariena J. A. van der Plas, Martin Malmsten, Thomas Rades, Andrea Heinz
Summary: Nanofiber-reinforced hydrogels have attracted attention in biomedical engineering due to their mechanical strength, water absorption properties, and potential for promoting cell migration. This study demonstrates a one-step solution for producing nanofiber-reinforced hydrogels using zein and gelatin proteins. The resulting nanofiber-hydrogel composite showed significant improvements in swelling and physical integrity compared to hydrogels without nanofibers. Additionally, the incorporation of antimicrobial agents into the fibers proved effective against Staphylococcus aureus and Escherichia coli.
Article
Pharmacology & Pharmacy
Cristina Garcia-Mouton, Elisa Parra-Ortiz, Martin Malmsten, Antonio Cruz, Jesus Perez-Gil
Summary: This study evaluates the interaction between pulmonary surfactant (PS) and antimicrobial peptides (AMPs), and finds that PS can be used to transport AMPs without interfering with their function. The combination of PS and AMPs improves their delivery, increases antimicrobial effects, and reduces toxicity on airway epithelial cells.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Engineering, Biomedical
Heidi Kyung Noddeland, Marianne Lind, Louise Bastholm Jensen, Karsten Petersson, Tine Skak-Nielsen, Flemming Hofmann Larsen, Martin Malmsten, Andrea Heinz
Summary: This study developed enzyme-responsive hydrogels for the delivery of anti-inflammatory drugs for the treatment of inflammatory skin diseases. The hydrogels were crosslinked with polyethylene glycols using peptide linkers that were sensitive to matrix metalloproteinases. The drug release was triggered by matrix metalloproteinases and depended on the type of crosslinker and polyethylene glycol polymer.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
D. Aurora Perini, Elisa Parra-Ortiz, Inmaculada Varo, Maria Queralt-Martin, Martin Malmsten, Antonio Alcaraz
Summary: The interaction between nanoplastics and phospholipid membranes can affect membrane integrity and lead to toxic effects.
Editorial Material
Pharmacology & Pharmacy
Artur Schmidtchen, Haris Mirza, Mariena J. A. van der Plas, Aftab Nadeem, Manoj Puthia
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Heidi K. Noddeland, Marianne Lind, Karsten Petersson, Frank Caruso, Martin Malmsten, Andrea Heinz
Summary: Protease-responsive multi-arm polyethylene glycol-based microparticles with biscysteine peptide crosslinkers (CGPGG↓LAGGC) were developed for intradermal drug delivery. The microparticles showed suitable size and dispensability for skin injections. Exposure to matrix metalloproteinase 9 (MMP-9) resulted in partial network destruction and decreased elastic moduli, while multiple-time exposure showed increased release of tofacitinib citrate from the MMP-responsive microparticles. The complexity of the polyethylene glycol building blocks could tune not only the release profile but also the elastic moduli, and the cytotoxicity studies showed no reduction in metabolic activity.
Article
Chemistry, Physical
You Xu, Elisa Parra-Ortiz, Feng Wan, Olga Canadas, Begona Garcia-Alvarez, Aneesh Thakur, Henrik Franzyk, Jesus Perez-Gil, Martin Malmsten, Camilla Foged
Summary: Pulmonary delivery of siRNA using nanoparticle-based systems shows promise for treating respiratory diseases. We developed inhaler formulations of siRNA-loaded lipid-polymer hybrid nanoparticles and optimized them for inhalation therapy. The interaction between the nanoparticles and pulmonary surfactant was studied to understand the mechanisms involved. The results provide new insights into the design criteria for nanoparticle transport through the air-blood barrier.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Meeting Abstract
Biophysics
Deborah Aurora Perini, Elisa Parra-Ortiz, Inmaculada Varo, Maria Queralt-Martin, Martin Malmsten, Antonio Alcaraz
BIOPHYSICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Elisa Parra-Ortiz, Lucrezia Caselli, Monica Agnoletti, Maximilian W. A. Skoda, Xiaomin Li, Dongyuan Zhao, Martin Malmsten
Summary: This study investigates the interaction between mesoporous silica and photocatalytic titanium dioxide nanoparticles with lipid membranes. The results show that the incorporation of TiO2 nanoparticles into the silica nanoparticles improves their colloidal stability and retains their ability to destabilize lipid membranes under UV-illumination. The study provides new mechanistic information on the loading of photocatalytic nanomaterials for increased performance.