Article
Medicine, General & Internal
Roberta Amoriello, Maria Chernigovskaya, Victor Greiff, Alberto Carnasciali, Luca Massacesi, Alessandro Barilaro, Anna M. Repice, Tiziana Biagioli, Alessandra Aldinucci, Paolo A. Muraro, David A. Laplaud, Andreas Lossius, Clara Ballerini
Summary: CSF repertoires in MS patients show a significantly higher percentage of public clones and sequence similarity compared to peripheral blood, but the repertoire polarization between CSF and PB is not significantly different. One PPMS CSF repertoire differs in TCR similarity architecture. Cluster analysis distinguishes MS from HD.
Article
Multidisciplinary Sciences
Rohit Arora, Ramy Arnaout
Summary: This paper presents a framework for describing repertoires based on the epitope-binding properties of antibodies and TCRs. The framework has the potential to distinguish specific immune responses, identify differences in immune function among different groups, and has important implications for immune function assessment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
John T. Keane, Avery D. Posey
Summary: CAR T-cell therapy utilizes chimeric antigen receptors to recognize tumor surface antigens, and can potentially target a diverse range of macromolecules to enhance antigen diversity and tumor specificity in cancer treatment.
Article
Multidisciplinary Sciences
Hagit Philip, Tom Snir, Miri Gordin, Mikhail Shugay, Alona Zilberberg, Sol Efroni
Summary: This study quantified the dynamics of the T cell receptor repertoire during treatment with anti-CTLA-4 in a mouse model for colorectal cancer, revealing that the response to treatment is closely connected to shared and public parts of the T cell repertoire, dominated by specific motifs and time-dependent behaviors of specific TCR sequences. The research also emphasizes the importance of temporal analyses of the immune response, showing that a specific time point might be useful in assessing the true response to treatment.
Review
Biochemistry & Molecular Biology
Eimear M. Foley, Joel T. T. Parkinson, Ruth E. E. Mitchell, Lorinda Turner, Golam M. M. Khandaker
Summary: This study systematically reviewed and meta-analyzed white blood cell subsets in patients with depression, revealing widespread alterations in circulating myeloid and lymphoid cells, indicating immune dysfunction in depression. These immune cells could be potential biomarkers for patient stratification in future immunotherapy trials for depression.
MOLECULAR PSYCHIATRY
(2023)
Article
Biochemistry & Molecular Biology
Kathrin Jansen, Noriko Shikama-Dorn, Moustafa Attar, Stefano Maio, Maria Lopopolo, David Buck, Georg A. Hollander, Stephen N. Sansom
Summary: Thymic epithelial cells (TEC) play a crucial role in controlling the selection of T cells reactive to pathogens. A study found that mature mTEC express a higher percentage of protein-coding transcripts compared to peripheral tissues, but produce fewer isoforms for genes with tissue-restricted expression. Furthermore, it was discovered that TEC utilize a small number of peripheral splicing factors, including members of the RBFOX family, to generate a broad but selective representation of the peripheral splice isoform repertoire.
Article
Immunology
Hanspeter Pircher, Daniel D. Pinschewer, Thomas Boehm
Summary: Previous studies have questioned the effectiveness of thymic-negative selection in identifying self-specific T cells using peptide-MHC tetramers. In this study, we used pMHCI tet to enumerate CD8 T cells specific for the gp33 epitope of lymphocytic choriomeningitis virus in a mouse model. We found that the gp33-tet staining profiles of polyclonal T cells from GP(+) and GP(-) mice were overlapping, but cells from GP(+) mice had lower mean fluorescence intensities.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Marte Heimli, Siri Tennebo Flam, Hanne Sagsveen Hjorthaug, Pal Marius Bjornstad, Maria Chernigovskaya, Quy Khang Le, Xavier Tekpli, Victor Greiff, Benedicte Alexandra Lie
Summary: The study indicates that the adaptive immune receptor repertoire may influence the differentiation of thymocytes, providing insights for the development of therapies for immune-mediated diseases.
SCIENTIFIC REPORTS
(2023)
Review
Immunology
Antonella Cardinale, Carmen Dolores De Luca, Franco Locatelli, Enrico Velardi
Summary: The recognition and immune response against tumor antigens by T cells depends on the diversity of the T-cell receptor (TCR) repertoire, which can be impaired by immunological insults and aging-related decline in thymus function. Manipulating thymic function to enhance anti-tumor immunity is crucial in improving response to cancer immunotherapy, especially checkpoint blockade therapy. The limited response rate to checkpoint blockade therapy may be linked to suboptimal TCR repertoire in cancer patients prior to therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biology
Johannes Truck, Anne Eugster, Pierre Barennes, Christopher M. Tipton, Eline T. Luning Prak, Davide Bagnara, Cinque Soto, Jacob S. Sherkow, Aimee S. Payne, Marie-Paule Lefranc, Andrew Farmer, Magnolia Bostick, Encarnita Mariotti-Ferrandiz
Summary: The use of adaptive immune receptor repertoire sequencing (AIRR-seq) has provided new insights into the immune system but also comes with challenges. The AIRR Community's Biological Resources Working Group conducted a survey to understand the need for standards and controls in generating and annotating AIRR-seq data, and offered recommendations for developing AIRR-seq standards and controls based on the survey results.
Article
Immunology
Augustina Frimpong, Michael Fokuo Ofori, Abdoelnaser M. Degoot, Kwadwo Asamoah Kusi, Buri Gershom, Jacob Quartey, Eric Kyei-Baafour, Nhi Nguyen, Wilfred Ndifon
Summary: This study conducted a detailed comparative analysis of the T cell repertoire in African children with and without clinical malaria, revealing that children with symptomatic malaria have lower TCR diversity and frequency of shared TCR sequences. Exposure to P. falciparum antigens during malaria disrupts the adaptive immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Young-Seon Mederacke, Mikalai Nienen, Michael Jarek, Robert Geffers, Katharina Hupa-Breier, Nina Babel, Petra Reinke, Ingmar Mederacke, Florian Wolfgang Rudolf Vondran, Danny Jonigk, Heiner Wedemeyer, Elmar Jaeckel
Summary: This study compared TCR repertoires between liver grafts and blood in liver transplant recipients with different rejection statuses. The results revealed that donor-specific T cells clonally expanded and accumulated in the transplanted liver, with distinct TCR repertoires between the liver and peripheral blood. This highlights the importance of conducting protocol liver biopsies to investigate intragraft immune responses for allo-directed immune responses.
JOURNAL OF HEPATOLOGY
(2021)
Article
Oncology
Meridith L. Balbach, Margaret L. Axelrod, Justin M. Balko, Armand Bankhead, Tristan Shaffer, Lee Lim, Jiannan Guo, Jennifer Hernandez, Mark Li, Wade T. Iams
Summary: This study investigates the relationship between peripheral T-cell receptor (TCR) diversity and clinical outcomes in small cell lung cancer (SCLC) patients. Results show no significant associations, highlighting the need for further research.
TRANSLATIONAL LUNG CANCER RESEARCH
(2023)
Article
Multidisciplinary Sciences
Miko Valori, Joonas Lehikoinen, Lilja Jansson, Jonna Clancy, Sofie A. Lundgren, Satu Mustjoki, Pentti Tienari
Summary: Somatic mutations in the STAT3 SH2 domain are found in CD8+ cells in multiple sclerosis (MS) patients and controls, with a prevalence of 26%. The mutations are associated with older age and lower neutrophil counts. There is no significant difference in the mutation prevalences between MS patients and controls.
Review
Immunology
Svenja Foth, Sara Voelkel, Daniel Bauersachs, Michael Zemlin, Chrysanthi Skevaki
Summary: A newborn must develop cellular immunity to defend against pathogens, with dysregulation leading to severe disorders. This review discusses the role of T cell immunity in antigen detection, characteristics of mature TCR repertoire, and assessments methods. Various early childhood disorders and changes in the TCR repertoire are outlined, along with the latest findings and research gaps.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biophysics
Fred Falkenburg, Eliana Ruggiero, Chaira Bonini, David Porter, Jeff Miller, Floran Malard, Mohamad Mohty, Nicolaus Kroger, Hans Jochem Kolb
BONE MARROW TRANSPLANTATION
(2019)
Article
Rheumatology
Beatrice C. Cianciotti, Eliana Ruggiero, Corrado Campochiaro, Giacomo Oliveira, Zulma I. Magnani, Mattia Baldini, Matteo Doglio, Michela Tassara, Angelo A. Manfredi, Elena Baldissera, Fabio Ciceri, Nicoletta Cieri, Chiara Bonini
ARTHRITIS & RHEUMATOLOGY
(2020)
Review
Immunology
Francesco Manfredi, Beatrice Claudia Cianciotti, Alessia Potenza, Elena Tassi, Maddalena Noviello, Andrea Biondi, Fabio Ciceri, Chiara Bonini, Eliana Ruggiero
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Maria Xydia, Raheleh Rahbari, Eliana Ruggiero, Iain Macaulay, Maxime Tarabichi, Robert Lohmayer, Stefan Wilkening, Tillmann Michels, Daniel Brown, Sebastiaan Vanuytven, Svetlana Mastitskaya, Sean Laidlaw, Niels Grabe, Maria Pritsch, Raffaele Fronza, Klaus Hexel, Steffen Schmitt, Michael Mueller-Steinhardt, Niels Halama, Christoph Domschke, Manfred Schmidt, Christof von Kalle, Florian Schuetz, Thierry Voet, Philipp Beckhove
Summary: Regulatory T cells in breast cancer do not show clonal relationship with their circulating counterpart, but share a common origin with intratumoral antigen-experienced conventional T cells.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Francesco Manfredi, Danilo Abbati, Beatrice Claudia Cianciotti, Lorena Stasi, Alessia Potenza, Eliana Ruggiero, Zulma Magnani, Erica Carnevale, Matteo Doglio, Maddalena Noviello, Elena Tassi, Chiara Balestrieri, Silvia Buonanno, Francesca Clemente, Claudia De Lalla, Maria Pia Protti, Anna Mondino, Giulia Casorati, Paolo Dellabona, Chiara Bonini
Summary: The article introduced a new tool called CytoChain for analyzing dynamic and complex flow cytometry samples to help researchers identify stem-like memory cells (T-SCM) suitable for adoptive T-cell therapy. By applying CytoChain, researchers successfully discovered a previously unidentified cell feature with superior expansion potential, providing support for new immunophenotypes in immunotherapy and patient immunomonitoring.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Cell Biology
Eliana Ruggiero, Erica Carnevale, Aaron Prodeus, Zulma Irene Magnani, Barbara Camisa, Ivan Merelli, Claudia Politano, Lorena Stasi, Alessia Potenza, Beatrice Claudia Cianciotti, Francesco Manfredi, Mattia Di Bono, Luca Vago, Michela Tassara, Sara Mastaglio, Maurilio Ponzoni, Francesca Sanvito, Dai Liu, Ishina Balwani, Rossella Galli, Marco Genua, Renato Ostuni, Matteo Doglio, Daniel O'Connell, Ivy Dutta, Stephanie Ann Yazinski, Mark McKee, Mohamed Simo Arredouani, Birgit Schultes, Fabio Ciceri, Chiara Bonini
Summary: TCR-based therapy has the potential to induce durable clinical responses in cancer patients by targeting tumor antigens and promoting T cell survival. Isolated TCRs specific for WT1 and engineered T cells have shown effective killing of tumor cells, making them a promising candidate for immunotherapy.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biology
Sara Feola, Jacopo Chiaro, Beatriz Martins, Salvatore Russo, Manlio Fusciello, Erkko Yloesmaeki, Chiara Bonini, Eliana Ruggiero, Firas Hamdan, Michaela Feodoroff, Gabriella Antignani, Tapani Viitala, Sari Pesonen, Mikaela Groenholm, Rui M. M. Branca, Janne Lehtioe, Vincenzo Cerullo, Ping-Chih Ho
Summary: We describe a streamlined pipeline for generating personalized cancer vaccines, starting from the isolation and selection of immunogenic peptide candidates expressed on tumor cells. The pipeline was validated in a mouse colon tumor model, confirming its feasibility and control of tumor lesions.
Article
Medicine, Research & Experimental
Fabio Russo, Eliana Ruggiero, Rosalia Curto, Laura Passeri, Francesca Sanvito, Ileana Bortolomai, Anna Villa, Silvia Gregori, Andrea Annoni
Summary: The study developed a gene transfer approach to modify the T cell repertoire in thymic epithelial cells, correcting the autoimmune response in type 1 diabetes. The strategy involved intrathymic injection of a lentiviral vector to achieve stable transgene expression in thymic epithelial cells, leading to successful correction of the disease in mice models.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Review
Oncology
Emmanuel Donnadieu, Maik Luu, Miriam Alb, Brigitte Anliker, Silvia Arcangeli, Chiara Bonini, Biagio De Angelis, Rashmi Choudhary, David Espie, Anne Galy, Cam Holland, Zoltan Ivics, Chahrazade Kantari-Mimoun, Marie Jose Kersten, Ulrike Koehl, Chantal Kuhn, Bruno Laugel, Franco Locatelli, Ibtissam Marchiq, Janet Markman, Marta Angiola Moresco, Emma Morris, Helene Negre, Concetta Quintarelli, Michael Rade, Kristin Reiche, Matthias Renner, Eliana Ruggiero, Carmen Sanges, Hans Stauss, Maria Themeli, Jan Van den Brulle, Michael Hudecek, Monica Casucci
Summary: The article introduces current preclinical models used to test the safety of engineered T cells, emphasizes the limitations of existing models, and proposes potential measures for improvement.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Oncology
Sonia Guedan, Maik Luu, Delphine Ammar, Paula Barbao, Chiara Bonini, Philippe Bousso, Christian J. Buchholz, Monica Casucci, Biagio De Angelis, Emmanuel Donnadieu, David Espie, Beatrice Greco, Richard Groen, Johannes B. Huppa, Chahrazade Kantari-Mimoun, Bruno Laugel, Mary Mantock, Janet L. Markman, Emma Morris, Concetta Quintarelli, Michael Rade, Kristin Reiche, Alba Rodriguez-Garcia, Juan Roberto Rodriguez-Madoz, Eliana Ruggiero, Maria Themeli, Michael Hudecek, Ibtissam Marchiq
Summary: Immunotherapy with gene engineered CAR and TCR transgenic T-cells is a groundbreaking treatment in cancer medicine, and has the potential to be used in common solid tumors. However, in order to improve and accelerate the selection of lead T-cell products for clinical translation, it is necessary to assess preclinical models and develop new models with higher predictive value.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Michele Tomasi, Elena Caproni, Mattia Benedet, Ilaria Zanella, Sebastiano Giorgetta, Mattia Dalsass, Enrico Koenig, Assunta Gagliardi, Laura Fantappie, Alvise Berti, Silvia Tamburini, Lorenzo Croia, Gabriele Di Lascio, Erika Bellini, Silvia Valensin, Giada Licata, Guido Sebastiani, Francesco Dotta, Federica Armanini, Fabio Cumbo, Francesco Asnicar, Aitor Blanco-Miguez, Eliana Ruggiero, Nicola Segata, Guido Grandi, Alberto Grandi
Summary: A growing body of evidence supports the important role of the gut microbiome in cancer immunity, and it has been found that microbiome proteins can induce specific T cells, which can inhibit tumor growth. Furthermore, personalized mucosal cancer vaccines can be developed using engineered microbes and Outer Membrane Vesicles.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Martina Musella, Andrea Guarracino, Nicoletta Manduca, Claudia Galassi, Eliana Ruggiero, Alessia Potenza, Ester Maccafeo, Gwenola Manic, Luca Mattiello, Sara Soliman Abdel Rehim, Michele Signore, Marco Pietrosanto, Manuela Helmer-Citterich, Matteo Pallocca, Maurizio Fanciulli, Tiziana Bruno, Francesca De Nicola, Giacomo Corleone, Anna Di Benedetto, Cristiana Ercolani, Edoardo Pescarmona, Laura Pizzuti, Francesco Guidi, Francesca Sperati, Sara Vitale, Daniele Macchia, Massimo Spada, Giovanna Schiavoni, Fabrizio Mattei, Adele De Ninno, Luca Businaro, Valeria Lucarini, Laura Bracci, Eleonora Arico, Giovanna Ziccheddu, Francesco Facchiano, Stefania Rossi, Massimo Sanchez, Alessandra Boe, Mauro Biffoni, Ruggero De Maria, Ilio Vitale, Antonella Sistigu
Summary: The study demonstrates that type I interferons can promote the formation of cancer stem cells by upregulating the chromatin remodeling factor KDM1B. Inhibition of KDM1B could potentially prevent stem cell expansion and increase the long-term benefit of therapy.
Meeting Abstract
Oncology
Alessia Potenza, Chiara Bonini, Chiara Balestrieri, Luca Albarello, Federica Pedica, Martina Spiga, Francesco Manfredi, Beatrice Cianciotti, Claudia De Lalla, Lorena Stasi, Elena Tassi, Silvia Bonfiglio, Giulia Scotti, Miriam Redegalli, Donatella Biancolini, Danilo Abbati, Fabio Simeoni, Dejan Lazarevic, Ugo Elmore, Guido Fiorentini, Giulia Di Lullo, Giulia Casorati, Paolo Dellabona, Claudio Doglioni, Giovanni Tonon, Riccardo Rosati, Luca Aldrighetti, Eliana Ruggiero
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Meeting Abstract
Oncology
Alessia Potenza, Chiara Balestrieri, Luca Albarello, Federica Pedica, Lorena Stasi, Francesco Manfredi, Martina Spiga, Elena Tassi, Beatrice Claudia Cianciotti, Danilo Abbati, Ugo Elmore, Andrea Biondi, Luca Aldrighetti, Claudia De Lalla, Giulia Di Lullo, Paolo Dellabona, Eliana Ruggiero, Riccardo Rosati, Chiara Bonini
Meeting Abstract
Biotechnology & Applied Microbiology
Fabio Russo, Eliana Ruggiero, Rosalia Curto, Laura Passeri, Francesca Sanvito, Ileana Bortolomai, Anna Villa, Silvia Gregori, Andrea Annoni