Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 417, Issue -, Pages 134-138Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2015.01.004
Keywords
CD8+T cells; Activation; Immunotherapy
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Funding
- European Foundation for the Study of Diabetes
- Medical Research Council (UK) [G0901155]
- Medical Research Council [G0901155] Funding Source: researchfish
- MRC [G0901155] Funding Source: UKRI
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Murine adoptive CD8+ T-cell immunotherapy studies require the generation of large numbers of high viability CD8+ cells. Here we report a tissue culture protocol for the reliable expansion of CD8+ T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells were transfected with an mRNA GFP construct and transferred into NOD mice. GFP positive cells could be detected 7 days after transfer thus confirming that the cells survive and are functional for up to 1 week. (C) 2015 The Authors. Published by Elsevier B.V.
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