Journal
CHINESE JOURNAL OF CHEMICAL ENGINEERING
Volume 18, Issue 5, Pages 870-875Publisher
CHEMICAL INDUSTRY PRESS
DOI: 10.1016/S1004-9541(09)60141-5
Keywords
immobilized lipase; transesterification; rice bran oil; methanolysis; optimization
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Funding
- National High Technology Research and Development Program of China [2006AA020101, 2007AA10Z360, 2009AA03Z232]
- National Science & Technology Pillar Program [2008BA163B07]
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The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent, number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were: 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 degrees C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.
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