Journal
CHINESE JOURNAL OF CANCER RESEARCH
Volume 22, Issue 2, Pages 141-147Publisher
CHINESE JOURNAL CANCER RESEARCH CO
DOI: 10.1007/s11670-010-0141-5
Keywords
Triptolide; RPMI-8226 cells; Cell cycle; Multiple myeloma
Categories
Funding
- National Natural Science Foundation of China [30700882]
- Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Ask authors/readers for more resources
To investigate the effects of triptolide(TPL) on cell growth, cell cycle and the expressions of p21wap1/cip1 and p27kip1. MTT assay was used to determine the cell viability after triptolide treatment in human multiple myeloma RPMI-8226 cells. The effect on cell cycle distribution was determined by flow cytometry. Semi-quantitative reverse transcription-PCR was used to examine the mRNA expressions of p21wap1/cip1 and p27kip1. The protein expressions of p21 wap1/cip1 and p27kip1 were determined by Western blot. Triptolide of varying concentrations induced cell viability inhibition in dose- and time-related fashion and caused G(0)-G(1) phase arrest of cell cycle progression in RPMI-8226 cells. These effects accompanied with up-modulation of the expressions of p21 wap1/cip1 and p27kip1. These results suggest that triptolide inhibit cell proliferation and cell cycle progression via up-regulating p21wap1/cip1 and p27kip1 and triptolide may exert its anti-cancer activity through this pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available