Review
Pharmacology & Pharmacy
Shuting Ni, Rujing Chen, Kaili Hu
Summary: This review outlines the evolution of murine models for brainstem glioma (BG), one of the leading causes of pediatric death by brain tumors. The advantages and limitations of these models in drug discovery are discussed.
DRUG DISCOVERY TODAY
(2022)
Article
Chemistry, Multidisciplinary
Ying Xiong, Yiyu Zhang, Zefan Li, Md Shamim Reza, Xinyu Li, Xiaodong Tian, Hui-wang Ai
Summary: The NanoLuc luciferase and its furimazine substrate have greatly improved bioluminescence assays and imaging. However, their use for mammalian tissue imaging is limited by the low tissue penetration of blue photons. In this study, a mutant NLuc called QLuc was developed, which catalyzes the oxidation of a synthetic QTZ luciferin to produce bright and red-shifted emission. This red-shifted luciferase showed improved performance for imaging deep-tissue targets in live mice. Using QLuc, the researchers also demonstrated the use of immunoBLI for molecular imaging of tumor-associated antigens in a xenograft mouse model. QLuc is expected to have broad applications in noninvasive mammalian imaging, and immunoBLI serves as a convenient and nonradioactive molecular imaging tool for animal models in basic and preclinical research.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Cell Biology
Dian R. Arifin, Kenneth W. Witwer, Jeff W. M. Bulte
Summary: Extracellular vesicles (EVs) are mediators of intercellular signalling and play a key role in normal and pathological cellular processes. Non-invasive imaging methods allow for a better understanding of their biodistribution and pharmacokinetics, advancing their clinical applications.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biotechnology & Applied Microbiology
Giorgia Zambito, Gunja Mishra, Christopher Schliehe, Laura Mezzanotte
Summary: Melanoma is an aggressive type of skin cancer with a poor prognosis. Early detection is crucial for effective therapy. Recent research has shown that the interaction between melanoma cells and the immune system can aid in the development of efficient treatment strategies. This study used engineered macrophages as cell-based sensors to detect metastatic melanoma, demonstrating the potential of using optical imaging technologies to track circulating macrophages for non-invasive detection of the disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ryohei Saito-Moriya, Jun Nakayama, Genta Kamiya, Nobuo Kitada, Rika Obata, Shojiro A. Maki, Hiroshi Aoyama
Summary: Bioluminescence reactions are commonly used in optical in vivo imaging for quantifying tumor cells and gene expression. Researchers have developed various bioluminescence systems, including multicolor BLI based on artificial luciferin analogues and/or luciferase mutants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pediatrics
Asha Sarma, Josh M. Heck, Aashim Bhatia, Rekha S. Krishnasarma, Sumit Pruthi
Summary: The first article focuses on MRI techniques for brainstem pathologies and the normal anatomy, while the second article covers a broader range of diseases affecting the brainstem. The goal of both articles is to enhance the radiologist's ability to add clinical value in caring for pediatric patients with brainstem pathologies.
PEDIATRIC RADIOLOGY
(2021)
Article
Multidisciplinary Sciences
Charissa Poon, Petteri Teikari, Muhammad Febrian Rachmadi, Henrik Skibbe, Kullervo Hynynen
Summary: In this study, the first annotated dataset of rodent cerebrovasculature, MiniVess, was introduced. The dataset consists of 70 3D image volumes with segmented ground truths, created using various methods. The availability of annotated datasets is crucial for the development and testing of machine learning tools for biomedical image analysis.
Review
Genetics & Heredity
Nian Liu, Xiao Chen, Melanie A. Kimm, Matthias Stechele, Xueli Chen, Zhimin Zhang, Moritz Wildgruber, Xiaopeng Ma
Summary: Optical imaging technology has great potential in detecting inflammation, providing accurate molecular information and offering new insights into the diagnosis and therapeutic evaluation of inflammatory diseases. By using endogenous and exogenous contrast agents, optical imaging technology can accurately visualize sites of inflammation, enabling early disease detection and specific disease characterization.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Cell Biology
Sha Li, Sarah A. Mereby, Megan Rothstein, Matthew R. Johnson, Benjamin J. Brack, Ricardo Mallarino
Summary: The study presents a method called TIGER, which utilizes intraoviductal injecting technique and recombinant adeno-associated viruses (rAAVs) to deliver reagents into pregnant females for genome editing in non-traditional model species. The method shows high efficiency in generating knockout and knockin lines, and the use of a double-cleaving repair rAAV template significantly increases knockin frequency and germline transmission rates. The study suggests that TIGER could be a promising alternative to ex vivo genome editing methods and can be applied to a wide range of species.
Article
Biophysics
Atsushi Mahara, Keigo Shima, Raghav Soni, Ryutaro Onishi, Yoshiaki Hirano, Shigeyoshi Saito, Tetsuji Yamaoka
Summary: The study developed a supramolecular magnetic resonance contrast agent for long-term whole-tissue observation in a single individual. The results showed that microvessels formed inside cancer tissues at the early stage are leaky, but they form continuous microvessels after 12 days.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biology
Tobias Boothe, Mario Ivankovic, Markus A. Grohme, M. Andrea Markus, Christian Dullin, Xingbo Xu, Jochen C. Rink
Summary: Content-aware image restoration (CARE) is extended to luminescence imaging to overcome the limitation of longer exposure times, thus improving temporal resolution and throughput. Luminescent reporters, with inherently high signal-to-noise ratio, are powerful labeling tools for microscopy and in vivo imaging in biomedical research. However, the requirement of longer exposure times in luminescence imaging limits its application in high temporal resolution or throughput. This study demonstrates that CARE can significantly reduce the exposure time requirements in luminescence imaging.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Min Chen, Kaixiang Zhou, Sheng-Yao Dai, Sirimuvva Tadepalli, Preethi Bala Balakrishnan, Jinghang Xie, Fadi E. I. Rami, Tingting Dai, Liyang Cui, Juliana Idoyaga, Jianghong Rao
Summary: This study presents a bioluminescence probe, GBLI-2, for real-time imaging of granzyme B activity in tumors undergoing immune checkpoint inhibitor therapy. The GBLI-2 signal correlated with changes in the population of PD-1 and granzyme B-expressing CD8+ T cells in tumors.
CELL CHEMICAL BIOLOGY
(2022)
Article
Optics
Junsoo Lee, Hoseong Cho, Sungjo Park, Jaeyul Lee, Daewoon Seong, Ruchire Eranga Wijesinghe, Sangyeob Han, Shinheon Kim, Mansik Jeon, Jeehyun Kim
Summary: This study evaluates the cervicothoracic vasculature of mouse and rat specimens using a commercial PACT device, revealing various blood vessels and specific anatomical features that could be used as a reference for future preclinical experiments.
Article
Oncology
Yuan Xu, Irakliy Abramov, Evgenii Belykh, Giancarlo Mignucci-Jimenez, Marian T. Park, Jennifer M. Eschbacher, Mark C. Preul
Summary: This study compares the image quality and diagnostic performance of ex vivo and in vivo CLE imaging. The results show that in vivo images have higher brightness and contrast, and better image quality in glioma, meningioma, and intracranial metastatic tumor cases. In vivo imaging also yields higher sensitivity and negative predictive value than ex vivo imaging.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pathology
Kathryn L. Eschbacher, Cristiane M. Ida, Derek R. Johnson, Mohammed A. Alvi, Sarah M. Jenkins, Michael W. Ruff, Panagiotis Kerezoudis, Bryan J. Neth, Romela M. Pasion, David J. Daniels, Sani H. Kizilbash, Aditya Raghunathan
Summary: Research shows that adult patients with posterior fossa diffuse gliomas exhibit molecular heterogeneity, which may impact patient outcomes and treatment responses. The tumors can be classified into four distinct genetic groups, with most cases showing mutations in the driver genes. Patients with TERT promoter mutations have a shorter survival time compared to other subgroups.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Clinical Neurology
Arthur J. DiPatri, Simone Treiger Sredni, Gordan Grahovac, Tadanori Tomita
CHILDS NERVOUS SYSTEM
(2015)
Article
Rheumatology
Dong Xu, Chiang-Ching Huang, Akadia Kachaochana, Gabrielle A. Morgan, Maria F. Bonaldo, Marcelo B. Soares, Fabricio Costa, John Sarwark, Simone T. Sredni, Lauren M. Pachman
JOURNAL OF RHEUMATOLOGY
(2016)
Article
Pathology
Simone T. Sredni, Tadanori Tomita
PEDIATRIC AND DEVELOPMENTAL PATHOLOGY
(2015)
Article
Clinical Neurology
Simone Treiger Sredni, Chiang-Ching Huang, Mario Suzuki, Tatiana Pundy, Pauline Chou, Tadanori Tomita
CHILDS NERVOUS SYSTEM
(2016)
Article
Clinical Neurology
Jill Samis, Elio F. Vanin, Simone Treiger Sredni, Maria de Fatima de Bonaldo, Fabricio F. Costa, Tadanori Tomita, Reema Habiby, Donald Zimmerman, Marcelo B. Soares
CHILDS NERVOUS SYSTEM
(2016)
Article
Oncology
Mario Suzuki, Akihide Kondo, Ikuko Ogino, Hajime Arai, Tadanori Tomita, Simone Treiger Sredni
PEDIATRIC BLOOD & CANCER
(2017)
Article
Oncology
Simone Treiger Sredni, Mario Suzuki, Jian-Ping Yang, Jacek Topczewski, Anders W. Bailey, Tufan Gokirmak, Jeffrey N. Gross, Alexandre de Andrade, Akihide Kondo, David R. Piper, Tadanori Tomita
PEDIATRIC BLOOD & CANCER
(2017)
Article
Multidisciplinary Sciences
Sergey Malchenko, Simone Treiger Sredni, Yingtao Bi, Naira V. Margaryan, Jerusha Boyineni, Indra Mohanam, Tadanori Tomita, Ramana V. Davuluri, Marcelo B. Soares
Article
Oncology
Simone Treiger Sredni, Anders W. Bailey, Amreena Suri, Rintaro Hashizume, Xingyao He, Nundia Louis, Tufan Gokirmak, David R. Piper, Daniel M. Watterson, Tadanori Tomita
Article
Multidisciplinary Sciences
Sergey Malchenko, Simone Treiger Sredni, Hitoshi Hashimoto, Atsushi Kasai, Kazuki Nagayasu, Jianping Xie, Naira V. Margaryan, Kaoru Seiriki, Rishi R. Lulla, Richard E. B. Seftor, Lauren M. Pachman, Herbert Y. Meltzer, Mary J. C. Hendrix, Marcelo B. Soares
Article
Biochemistry & Molecular Biology
Amreena Suri, Anders W. Bailey, Mauricio T. Tavares, Hendra Gunosewoyo, Connor P. Dyer, Alex T. Grupenmacher, David R. Piper, Robert A. Horton, Tadanori Tomita, Alan P. Kozikowski, Saktimayee M. Roy, Simone T. Sredni
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Pathology
Josef Rueschoff, Michael Friedrich, Iris Nagelmeier, Matthias Kirchner, Lena M. Andresen, Karin Salomon, Bryce Portier, Simone T. Sredni, Hans Ulrich Schildhaus, Bharat Jasani, Marius Grzelinski, Giuseppe Viale
Summary: This study compared the performance of two different HER2 detection assays in breast cancer samples and found a high concordance between the results. The HercepTest (mAb) assay showed a tendency to overscore HER2 status in some cases, but demonstrated higher sensitivity for tumors with gene amplification and HER2-low expression. Further research will explore whether this assay can improve patient selection, particularly for new HER2-directed therapies.
Article
Biochemistry & Molecular Biology
Shahinda S. R. Alsayed, Amreena Suri, Anders W. Bailey, Samuel Lane, Eryn L. Werry, Chiang-Ching Huang, Li-Fang Yu, Michael Kassiou, Simone Treiger Sredni, Hendra Gunosewoyo
Summary: This study focused on the antitumor activities of several indole compounds against pediatric glioblastomas, showing significant inhibitory effects of compounds 8a, 8c, 8f, 12c, and 24d on KNS42 cells. Compounds 8f and 24d exhibited antagonistic effects on CB1R and CB2R, while compounds 8a, 8c, and 12c did not activate or antagonize these receptors.
RSC MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Shahinda S. R. Alsayed, Shichun Lun, Anders W. Bailey, Amreena Suri, Chiang-Ching Huang, Mauro Mocerino, Alan Payne, Simone Treiger Sredni, William R. Bishai, Hendra Gunosewoyo
Summary: The study focused on designing, synthesizing, and evaluating indoleamide analogues for their antitubercular and antitumour activities, with compounds 8g and 8f showing promising activity, while compounds 9a and 15 exhibited potent cytotoxic and antiproliferative effects.
Letter
Clinical Neurology
Simone Treiger Sredni, Tadanori Tomita
CHILDS NERVOUS SYSTEM
(2017)