Journal
CHEMPHYSCHEM
Volume 15, Issue 4, Pages 750-755Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201301004
Keywords
fluorophores; live-cell imaging; rhodamine; single-molecule studies; superresolution microscopy
Funding
- NCCR Chemical Biology
- European Research Council [243016]
- European Research Council (ERC) [243016] Funding Source: European Research Council (ERC)
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Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can result in up to ten-fold higher densities of single molecule localizations, and more photons per localization as compared with unmodified dyes. These two parameters directly impact the achievable resolution, and we see a significant improvement in the quality of live-cell point-localization super-resolution images taken with reduced dyes. These improvements carry over to increase the density of trajectories for single-molecule tracking experiments.
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