Journal
CHEMPHYSCHEM
Volume 11, Issue 13, Pages 2887-2895Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201000210
Keywords
Debye length; DNA; electrochemical impedance spectroscopy; hybridization; self-assembled monolayers
Funding
- EU [MEST-CT-2005-020681]
- Ruhr-University Research School [DFG GSC 98/1]
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Determination of DNA hybridization at elctrode surfaces modified with thiol tethered single-stranded DNA (ssDNA) capture probes and co-assembled with short-chain thiol derivatives using electrochemical impedance spectroscopy requires a careful design of the electrode/electrolyte interface as well as an in-depth understanding of the processes at the interface during DNA hybridization. The influence of the electrode potential, the ssDNA coverage, the ionic strength, the nature of the thiol derivative and especially the Debye length are shown to have a significant impact on the impedance spectra. A mixed monolayer comprising-in addition to the ssDNA capture probe-both mercaptohexanol (MCH) and mercaptopropionic acid (MPA) is suggested as an interface design which allows a high efficiency of the DNA hybridization concomitantly with a reliable modulation of the charge-transfer resistance of the electrode upon hybridization.
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