Environmentally relevant concentrations of the flame retardant tris(1,3-dichloro-2-propyl) phosphate change morphology of female zebrafish

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) was considered as a re-emerging environmental pollutant, and accumulated evidences suggested that it was global distributed in various environmental media. However, effect of TDCIPP on fish morphology remained largely unknown. In this study, one month old zebrafish (Danio rerio) were exposed to 0, 0.05, 0.5 or 5 mu g/L TDCIPP for 120 days, and effects on fish morphology and expressions of genes included in the development of muscle and bone were examined. Using landmark-based geometric morphometrics, we found that environmentally relevant concentrations of TDCIPP altered morphology of female zebrafish. Furthermore, TDCIPP decreased the ratio of caudal fin area to whole body area and muscle density. These effects were possibly resulted from the alteration in the expression of genes included in the development of muscle (myf5 and myog) and bone (bmp2b and bmp4). (C) 2018 Elsevier Ltd. All rights reserved.