Changes in hypopharyngeal glands of nurse bees (Apis mellifera) induced by pollen-containing sublethal doses of the herbicide Roundup (R)

Decreasing pollinator populations worldwide has generated great concern and stimulated countless studies to understand the origin of colony losses. One main cause is the indiscriminate use of different pesticides, producing subtle negative effects on bee physiology and behavior. Royal jelly synthesized in the hypopharyngeal glands is an essential protein for feeding all individuals of the hive, especially the queen. Therefore, the present study aimed to determine the effect of sublethal concentrations of Roundup (R) on the hypopharyngeal glands of nursing workers, including its interference with the production of royal jelly. The herbicide was found to promote changes in the cellular ultrastructure of these glands, causing early degeneration of the rough endoplasmic reticulum and morphological and structural changes in the mitochondria. No changes were noted in the amount of royal jelly produced, but additional long-term studies are necessary to determine possible qualitative changes. This is the first study to evaluate the effect of Roundup (R) on the royal jelly-producing glands, showing that resultant alterations in these structures can trigger damage to the development and survival of bee colonies. (C) 2018 Elsevier Ltd. All rights reserved.