Article
Biochemistry & Molecular Biology
Da Seul Kong, Changman Kim, Young Eun Song, Jiyun Baek, Hyeon Sung Im, Jung Rae Kim
Summary: In this study, ZVI was used as an external reducing agent to enhance the conversion of glycerol to 1,3-propanediol by K. pneumoniae L17. The production of 1,3-propanediol significantly increased with the use of ZVI, indicating that the application of ZVI can improve the production of high value-added platform chemicals.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Da Seul Kong, Minsoo Kim, Shuewi Li, Sakuntala Mutyala, Min Jang, Changman Kim, Jung Rae Kim
Summary: The bacterial redox state plays a crucial role in regulating the titer and yield of final metabolites in bioconversion processes. In this study, the use of zero-valent iron (ZVI) in glycerol bioconversion of Klebsiella pneumoniae L17 led to an increase in 1,3-propanediol (PDO) production and the cellular NADH/NAD+ ratio. The implementation of ZVI in the fermentation process shifted the bioconversion towards a more glycerol-reductive pathway. These findings suggest that ZVI can induce intracellular metabolic shifts and contribute to the biotransformation of PDO. Furthermore, this study provides insight into a novel microbial fermentation strategy involving microbially influenced corrosion (MIC).
FERMENTATION-BASEL
(2023)
Article
Energy & Fuels
Da Seul Kong, Eun Joo Park, Sakuntala Mutyala, Minsoo Kim, Yunchul Cho, Sang Eun Oh, Changman Kim, Jung Rae Kim
Summary: Crude glycerol, a major byproduct in biodiesel production, was successfully transformed into valuable 1,3-propanediol through bioconversion, with improvements in yield and biosynthesis efficiency achieved through the use of a bioelectrochemical system and zero-valent iron.
Article
Environmental Sciences
Jia Wang, Zuoming Xie, Yanxin Wang, Yang Yang, Mengna Chen
Summary: Metal-reducing bacteria, such as Bacillus D2201, play a crucial role in the biogeochemical cycle of As/Fe by facilitating the valence transition of Fe and As in groundwater environments. The addition of AQDS and As(V) significantly increases the release of Fe(II) and As(V) compared to AQDS-free treatments, indicating the importance of external factors in regulating Fe(III) reduction and As release in arsenic-resistant bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Agricultural Engineering
Shanshan Sun, Manping Zhang, Xushun Gu, Pan Yan, Shengbing He, Azharuddin Chachar
Summary: This review summarizes the discovery process, electron transfer mechanism, influencing factors and driven microorganisms of the newly discovered iron-nitrogen cycle process of microbial catalyzed NH4+ oxidation coupled with iron reduction (Feammox), and expounds the possibility and potential mechanism of Electron shuttles (ESs) to enhance Feammox efficiency. It highlights the current research situation of Feammox for nitrogen removal and future research directions, including how to apply ESs enhanced Feammox to promote nitrogen removal in practical wastewater treatment. This review provides new ideas for the engineering application research of Feammox and strong theoretical support for its development.
BIORESOURCE TECHNOLOGY
(2023)
Article
Infectious Diseases
You Lan, Mao Zhou, Xin Li, Xuan Liu, Jun Li, Wenen Liu
Summary: This study investigates the role of outer membrane vesicles (OMVs) and related proteins in iron acquisition in hypervirulent Klebsiella pneumoniae (HVKP) and classic Klebsiella pneumoniae (cKP). The results show that HVKP releases a significantly larger amount of OMVs in iron-deficient conditions and contains more proteins related to iron acquisition and transportation. This suggests a potential role for OMVs in the iron acquisition of HVKP.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Multidisciplinary Sciences
Meret Aeppli, Sebastien Giroud, Sanja Vranic, Andreas Voegelin, Thomas B. Hofstetter, Michael Sander
Summary: This study demonstrates that the variation in reduction rates of different iron oxides and extracellular electron shuttles (EES) can be explained by relating these rates to the free energy of the first electron transfer from the fully reduced EES to ferric iron oxide. This approach enhances our understanding of the controls on microbial iron oxide reduction by EES in anaerobic respiration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Infectious Diseases
Lucas Assoni, Raquel Girardello, Thiago Rojas Converso, Michelle Darrieux
Summary: Klebsiella pneumoniae is a bacterium causing serious infections with widespread antibiotic resistance, for which no vaccines are currently available; however, recombinant vaccines based on surface exposed bacterial antigens show promise as an alternative strategy.
INFECTIOUS DISEASES AND THERAPY
(2021)
Article
Microbiology
Philipp Grubwieser, Richard Hilbe, Clemens Michael Gehrer, Manuel Grander, Natascha Brigo, Alexander Hoffmann, Markus Seifert, Sylvia Berger, Igor Theurl, Manfred Nairz, Guenter Weiss
Summary: During infection, Klebsiella pneumoniae manipulates macrophage iron metabolism to acquire iron for sustained proliferation. This mechanism of microbe-mediated intracellular bacterial persistence provides insights for the development of novel antimicrobial therapies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Wilson H. W. Chu, Yi Han Tan, Si Yin Tan, Yahua Chen, Melvin Yong, David C. Lye, Shirin Kalimuddin, Sophia Archuleta, Yunn-Hwen Gan
Summary: Hypervirulent Klebsiella pneumoniae causes liver abscess and potential metastatic complications. We discovered a protein, IroP, on the virulence plasmid that suppresses the expression of type 3 fimbriae. Iron regulates hypermucoid capsule production and inversely regulates type 3 fimbriae expression through IroP. This plasmid-chromosomal cross talk confers an evolutionary advantage and contributes to the pathogen's success.
Review
Biochemistry & Molecular Biology
Vanessa Arato, Maria Michelina Raso, Gianmarco Gasperini, Francesco Berlanda Scorza, Francesca Micoli
Summary: Klebsiella pneumoniae is an opportunistic pathogen that has recently seen an increase in hypervirulent strains and multi-drug resistant clones. It has been prioritized as a critical antimicrobial resistance threat by the World Health Organization, leading to renewed interest in vaccine development and alternative treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Lei Wang, Na Liu, Lin Zhang, Likun Cui, Mengdie Zhu, Zhifang Li, Panpan Wang, Zhengbin Wang
Summary: This study aimed to evaluate the diagnostic accuracy of metagenomic next-generation sequencing (mNGS) for Klebsiella pneumoniae (Kp) bloodstream infections (BSI). The results showed that mNGS exhibits excellent accuracy for the diagnosis of Kp BSI, providing stronger diagnostic capabilities and optimizing treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Peng Lan, Ye Lu, Rushuang Yan, Lei Fang, Dongdong Zhao, Yan Jiang, Yunsong Yu, Xiaoxing Du, Jiancang Zhou
Summary: This study found that the Hmu system plays an important role in the growth of K. pneumoniae and is particularly critical in bloodstream infections. A novel typing scheme named hmST was established, and the genetic diversity of hmuRSTUV loci was analyzed based on a large number of genomes. This study provides new insights into identifying and monitoring the emergence of novel virulence evolution in K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Jaqueline Rocha, Catarina Ferreira, Dalila Mil-Homens, Antonio Busquets, Arsenio M. Fialho, Isabel Henriques, Margarita Gomila, Celia M. Manaia
Summary: This study compared the phenotypic, genotypic, and genomic characteristics of clinical and wastewater isolates of Klebsiella pneumoniae and found that they cannot be distinguished based on these features. Whole genome sequence analysis revealed that antibiotic and metal resistance or virulence genes were associated with mobile genetic elements, and these features varied among isolates according to their genetic lineage rather than their origin. Therefore, this study suggests that clinically relevant features of K. pneumoniae can be preserved in wastewater, even after treatment, highlighting the high capacity of K. pneumoniae for spreading through wastewater and increasing the risks of transmission back to humans.
Article
Chemistry, Multidisciplinary
Tania Sultana, Rebekah N. Duffin, Victoria L. Blair, Philip C. Andrews
Summary: In this study, a series of organometallic complexes of inactive quinolone antibiotics, nalidixic acid, oxolinic acid and norfloxacin, with gallium as the central metal ion, were investigated. These complexes exhibited antibacterial activity against four multi-drug resistant strains of Klebsiella pneumoniae, with a minimum inhibitory concentration (MIC) ranging from 0.024 to 0.781 μM.
CHEMICAL COMMUNICATIONS
(2023)
Article
Agriculture, Multidisciplinary
Songxiong Zhong, Xiaomin Li, Fangbai Li, Tongxu Liu, Dandan Pan, Yuhui Liu, Chengshuai Liu, Guojun Chen, Ruichuan Gao
Summary: Rice utilizes different strategies and sources to absorb iron depending on water conditions. Isotope signature combined with gene expression analysis revealed that under flooded conditions, rice predominantly absorbs Fe(III)-DMA from Fe plaque, while under drained conditions, it primarily absorbs Fe2+ from soil solution.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Environmental Sciences
Yating Chen, Xiaomin Li, Tongxu Liu, Fangbai Li, Weimin Sun, Lily Y. Young, Weilin Huang
Summary: In this study, microaerophilic Fe(II) oxidizing bacteria (FeOB) were enriched from paddy soil under microoxic conditions, and their capacity for Fe(II) oxidation and carbon assimilation was investigated. The results showed that the highest rate of Fe(II) oxidation was obtained in the FeCO3 tubes, and cells grown in the Fe3(PO4)2 tubes yielded the maximum assimilation amounts of 13C-NaHCO3 on Day 15.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Yufan Zhang, Xiangqin Wang, Yang Yang, Yingmei Huang, Xiaomin Li, Shiwen Hu, Kexue Liu, Yan Pang, Tongxu Liu, Fangbai Li
Summary: In order to assess the risks and develop remediation strategies for mercury-contaminated soils in paddy fields, understanding the mechanisms of mercury transformation and migration is crucial. This study investigated the changes in mercury fractions in a spiked acidic paddy soil under anoxic and oxic conditions, and established a kinetic model to quantitatively estimate the transformation processes. The results provided new insights into the exogenous mercury transformation processes and could be useful in predicting mercury availability in periodically flooded acidic paddy fields.
ENVIRONMENTAL POLLUTION
(2023)
Article
Agriculture, Multidisciplinary
Xiangqin Wang, Yanhong Du, Jing Zhou, Guanhong Chen, Xiaomin Li, Liping Fang, Fangbai Li, Yuzhen Yuan, Yang Yang, Fei Dou
Summary: The bioavailability of arsenic in paddy soil-rice systems is affected by ammonium fertilization, but the mechanisms controlling As transformation are not fully understood. This study found that urea and ammonium bicarbonate fertilizers significantly increased As(III) concentration in porewater, bioavailable As in rhizosphere soil, and the relative abundance of arrA and arsM genes. Moreover, the expression of As transporter genes in rice roots was upregulated, leading to increased translocation efficiency of As(III) from roots to brown rice. Therefore, attention should be paid to As-contaminated paddy fields with ammonium fertilization.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Environmental
Kaiyi Huang, Yang Yang, Hansha Lu, Shiwen Hu, Guojun Chen, Yanhong Du, Tongxu Liu, Xiaomin Li, Fangbai Li
Summary: This study investigates the transformation processes of nickel in paddy soils under anoxic and oxic conditions. The results show that under anoxic conditions, complexation with soil organic matter dominates nickel immobilization, while under oxic conditions, organic matter and Fe-Mn oxides contribute similarly to nickel release.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Mi Feng, Yanhong Du, Xiaomin Li, Fangbai Li, Jiangtao Qiao, Gongning Chen, Yingmei Huang
Summary: This study investigated the universality and characteristics of nitrate reduction coupled with arsenic (As) oxidation in paddy soils. The results showed that nitrate effectively transformed toxic As(III) into less toxic and nonlabile As(V), and the addition of nitrate increased the abundance of 16S rRNA and As(III) oxidase genes in the soils. Metagenomic analysis also identified several putative novel nitrate-dependent As(III)-oxidizing bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Songxiong Zhong, Xiaomin Li, Fangbai Li, Dandan Pan, Tongxu Liu, Yingmei Huang, Qi Wang, Haoming Yin, Fang Huang
Summary: The filling of rice grains is a critical period for the accumulation of excessive cadmium (Cd), but uncertainties still exist in identifying the sources of Cd enrichment in grains. In this study, pot experiments were conducted to investigate the transport and redistribution of Cd to grains during drainage and flooding. The results showed that Cd isotopes in rice plants were lighter than those in soil solutions but heavier than those in Fe plaques. Fe plaque was identified as a potential source of Cd in rice, particularly during flooding at the grain filling stage. Drainage during grain filling led to a negative fractionation of Cd from various parts of the rice plant, while flooding facilitated the transport of Cd from leaves, rachises, and husks to grains. These findings highlight the importance of ligands and transporters genes, along with isotope fractionation, in tracking the source of Cd transported to rice grains.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Geochemistry & Geophysics
Guoyong Huang, Xiaonan Wang, Dandan Pan, Guang Yang, Ruilin Zhong, Rumiao Niu, Bingqing Xia, Kuan Cheng, Tongxu Liu, Xiaomin Li
Summary: This study investigated the immobilization of cadmium (Cd) during nitrate-reducing Fe(II) oxidation (NRFO) and its impacts on the process. The results showed that higher initial Cd concentration led to a higher amount of Cd immobilized in precipitates but inhibited nitrate reduction, acetate metabolism, and cell growth. Cd had a minimal effect on Fe(II) oxidation during NRFO but slowed down the mineral transformation. Cd was found to be strongly co-localized with Fe on the surface of bacterial cells. Cd immobilization experiments indicated that both bacterial cells and secondary minerals were important for Cd immobilization. The findings provide important insights into the behavior and interactions of Cd during the NRFO process.
Article
Engineering, Environmental
Dandan Chen, Kuan Cheng, Tongxu Liu, Guojun Chen, Andreas Kappler, Xiaomin Li, Raymond Jianxiong Zeng, Yang Yang, Fujun Yue, Shiwen Hu, Fang Cao, Fangbai Li
Summary: This study demonstrates that dual N-O isotopes and kinetic modeling can be used to distinguish biological and chemical processes during microbially mediated NRFO. The main nitrate reductase in Acidovorax sp. strain BoFeN1 was found to be membrane-bound dissimilatory nitrate reductase (Nar). N-O isotopes can be used to differentiate between chemical and biological reactions, and chemodenitrification plays a more important role than biological reactions during the coupled process.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Songxiong Zhong, Liping Fang, Xiaomin Li, Tongxu Liu, Pei Wang, Ruichuan Gao, Guojun Chen, Haoming Yin, Yang Yang, Fang Huang, Fangbai Li
Summary: This study investigates the influence of chloride and sulfate ions on the transport of cadmium (Cd) in the soil-rice system. The results show that chloride and sulfate ions increase the concentration of Cd in the soil solution, Fe plaque, and rice plants, facilitating the mobility of Cd isotopes and contributing to Cd immobilization in the Fe plaque and uptake into roots. The findings provide insights into the role of anions in Cd migration and transformation in the soil-rice system.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Wenting Chi, Guojun Chen, Shiwen Hu, Xiaomin Li, Kuan Cheng, Qi Wang, Bingqing Xia, Yang Yang, Yibing Ma, Tongxu Liu
Summary: Paddy fields located around estuaries face the issue of seawater intrusion, and the impact of salinity levels on Cd accumulation in rice grains is still unclear. Pot experiments were conducted to investigate the effects of different salinity levels on Cd availability and uptake by rice plants. The results showed that higher salinity levels increased Cd availability and uptake in rice roots and grains, mainly due to competition for binding sites by cations and the formation of Cd complexation with anions. The study highlights the importance of considering food safety in rice cultivation near estuaries.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Zebin Hong, Shiwen Hu, Yang Yang, Ziwei Deng, Xiaomin Li, Tongxu Liu, Fangbai Li
Summary: Through investigating the variations of arsenic (As) in mine wastewater, it was found that under flooded condition, As(III) can be immobilized while As(V) can be activated in paddy soil, which influences the mobility of As. During drainage, available As is mainly immobilized by Fe oxyhydroxides and humic substances, and adsorbed As(III) is oxidized. These results provide an estimation pathway for the impacts of key biogeochemical cycles on exogenous As species under a redox-alternating condition.
Article
Environmental Sciences
Hansha Lu, Yang Yang, Kaiyi Huang, Guoyong Huang, Shiwen Hu, Dandan Pan, Tongxu Liu, Xiaomin Li
Summary: This study investigates the transformation of lead (Pb) in acidic soil under alternating anoxic-oxic conditions using a kinetic model. The results suggest that under anoxic conditions, Pb is gradually transferred to fulvic complex, Fe-Mn oxides bound, and sulfides bound Pb, while under oxic conditions, the fulvic complex Pb further increases. Phosphorus plays a more important role than organic matter in Pb immobilization under anoxic conditions, while the phosphates, Fe-Mn oxides, and sulfides immobilized Pb is slowly released and then complexed by fulvic acids during the re-immobilization of dissolved organic matter in soil under oxic conditions.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Multidisciplinary
Guoyong Huang, Dandan Pan, Milan Wang, Songxiong Zhong, Yingmei Huang, Fangbai Li, Xiaomin Li, Baoshan Xing
Summary: This study elucidated the regulatory mechanisms of Fe and Cd uptake in rice roots by FeNPs, including the effects of Fe plaque, gene expression, and NP accumulation. The application of FeNPs restricted the translocation of Fe to shoots, resulting in even lower Fe accumulation in shoots compared to those without FeNPs.
ENVIRONMENTAL SCIENCE-NANO
(2022)
Article
Chemistry, Multidisciplinary
Dandan Pan, Guoyong Huang, Jicai Yi, Jianghu Cui, Chuanping Liu, Fangbai Li, Xiaomin Li
Summary: The foliar application of silica nanoparticles to rice plants can reduce arsenic concentration in grains and restrict arsenic transport to leaves, husks, and grains. While silicon application does not decrease arsenic accumulation in the whole plant, it alters the distribution of arsenic in the plant.
ENVIRONMENTAL SCIENCE-NANO
(2022)
