Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/Salmonella and Allium test

Mutagenic and genotoxic effects of metolcarb were investigated by both bacterial reverse mutation assay in Salmonella typhimurium TA97, TA98. TA100 and TA102 strains with or without metabolic activation system (59) and Allium cepa root meristematic cells, respectively. Metolcarb was dissolved in DMSO in Ames/Salmonella test system. 0.1, 1 and 10 mu g/plate doses of metolcarb were found to be mutagenic S. typhimurium TA98 without 59. In Allium root growth inhibition test, EC50 value was determined 200 ppm and 0.5xEC(50), EC50 and 2xEC(50) concentrations of metolcarb were introduced to onion tuber roots and distilled water used as a negative control. Mitotic index (MI), increased in all concentrations compared to control at each exposure time. While disturbed anaphase-telophase, chromosome laggards, stickiness and bridges were observed in anaphase-telophase cells, pro-metaphase. C-mitosis, polyploidy, binuclear cells and disturbed nucleus were observed in other cells. The results were also analyzed statistically by using SPSS for Windows. Mann-Whitney test and Duncan's multiple range tests were performed respectively. (C) 2010 Elsevier Ltd. All rights reserved.