Can we use stable isotopes for ecotoxicological studies? Effect of DDT on isotopic fractionation in Perca fluviatilis

Nitrogen and carbon stable isotope analyses are frequently used to assess contaminant biomagnification in animals in the wild. Previous studies, mainly on plant but also on animal tissues, have shown that chemical stress can lead to shifts in delta N-15. In order to assess if an exposure to DDT at realistic concentration disrupted stable isotope signature in animals, delta N-15 and delta C-13 were studied in several tissues (liver, muscle, gill) of Perca fluviatilis fed with the same commercial diet uncontaminated or contaminated with DDT. We observed no DDT effect on the delta N-15 and delta C-13 of fish tissues. Our results show that stable isotopes can remain useful for field ecotoxicological studies despite food-chain contamination. However, correlations tions between the delta C-13 or delta N-15 values measured in the different organs were only found in DDT treated suggesting some disruption of major biochemical compound metabolism in tissues. fish, (C) 2009 Elsevier Ltd. All rights reserved.