Article
Chemistry, Multidisciplinary
Jaecheul Yu, Yeonju Kim, Jeongmi Kim, Soyeon Jeong, Seongjae Park, Taeho Lee
Summary: Anaerobic ammonium oxidation (anammox) is a nitrogen removal process with potential benefits, but maintaining stability is challenging. This study investigated the use of a respirometer for analyzing specific anammox activity (SAA), and found that the results were similar to those obtained using traditional methods. The simple SAA analysis provided by the respirometer can improve the operation, maintenance, and management of the anammox process.
APPLIED SCIENCES-BASEL
(2022)
Article
Agricultural Engineering
Shenhua Yang, Peiyuan Song, Baoyu Zhuang, Shujun Zhang, Xiaoyu Han, Yongzhen Peng, Jialin Li, Liang Zhang
Summary: This study demonstrates a novel method to enhance granulation by biofilm detachment in a plug-flow reactor for PN/A granular sludge process. The results suggest that granular sludge can replace biofilm effectively, with biofilm detachment favoring the granulation process significantly.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Zhibin Wang, Pengpeng Liu, Jing Zhou, Sherif Ismail, Shakeel Ahmad, Hanem M. M. Awad, Shou-Qing Ni
Summary: This study used biomagnetic effect to promote anammox process. Batch tests and long-term experiments showed that an appropriate electromagnetic field (EMF) strength can improve the nitrogen removal performance of anammox, but higher strengths can inhibit the activity of anammox bacteria. The addition of EMF with an appropriate strength greatly improved the nitrogen removal performance of granular sludge. The study also found that EMF addition can increase the relative abundance of the main anammox bacteria.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Engineering, Environmental
Kun Feng, Yu Lou, Yitian Li, Baiyun Lu, Anran Fang, Guojun Xie, Chuan Chen, Defeng Xing
Summary: The effect of conductive carriers on the formation of anammox biofilm and granules in anaerobic ammonium oxidation (anammox) process was investigated. The study found that the conductive carbon fiber brush (CB) carrier promoted the synchronous formation of biofilm and granules. The CB carrier allowed the reactor to operate at higher total nitrogen loading rate with lower concentrations of NH4+-N and NO2-N in the effluents.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Agricultural Engineering
Yan Guo, Yunzhi Qian, Junhao Shen, Yu Qin, Yu-You Li
Summary: This research successfully achieved synchronous nitrogen elimination and phosphorus recovery through the novel one-stage partial nitritation/anammox (PN/A)-hydroxyapatite (HAP) crystallization process. Within three months, high nitrogen removal rate and phosphorus removal efficiency were achieved, indicating the potential of this process in actual wastewater treatment. The coordination between biomass and mineral played an important role, providing guidance for practical application.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Victory Fiifi Dsane, Haejun Jeon, Yuri Choi, Sohee Jeong, Younggyun Choi
Summary: Magnetite is an iron-rich carrier that can enhance the activity and aggregation of anammox bacteria. In this study, different-sized samples from a granulation reactor were used, and it was found that larger granules had higher iron content and improved microbial cell density. The specific anammox activity increased with granule size, and the heme c content was also higher in larger granules. The extracellular polymeric substances (EPS) analysis showed differences in secretion intensity and protein structures among the different sizes. The relative abundance of Brocadia Sinica also increased with granule size. This study highlights the importance of in-depth EPS analysis beyond traditional yield and major content analysis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Dongdong Xu, Jiahui Fan, Wenji Li, Wenda Chen, Chao Pan, Da Kang, Yiyu Li, Shengdao Shan, Ping Zheng
Summary: The study investigated the correlation between permeability and granule size of Anammox granules, finding a negative relationship between them. With the increase of granule size, the pore structure varied significantly, affecting the fluid collection efficiency.
Review
Environmental Sciences
Lan Lin, Yanlong Zhang, Yu-You Li
Summary: This study reviews strategies for promoting the formation of anammox granules and the start-up of the anammox process. It introduces the formation mechanism of anammox granules, the operational performance of various strategies, and their promotion mechanisms. It provides guidance and reference for the cultivation of granular-anammox sludge, the start-up of the anammox process, and its practical application.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Weigang Wang, Hao Xue, Han Wang, Jie Ma, Min Wu, Yayi Wang
Summary: The study found that EPS extraction stimulated the adherence properties of all sludge samples. Anammox consortia exhibited the highest adhesion rate and mass, forming the most compact layer on sensor surfaces both before and after EPS extraction. The high adhesion ability of anammox granules is mainly attributed to their relatively high extracellular proteins/polysaccharides ratio.
ENVIRONMENTAL RESEARCH
(2021)
Article
Engineering, Environmental
Yujie Chen, Yan Guo, Gaoxuefeng Feng, Kampachiro Urasaki, Guangze Guo, Yu Qin, Kengo Kubota, Yu-You Li
Summary: The combination of Anammox and hydroxyapatite (HAP) granules has advantages in nitrogen and phosphorus removal and sludge settleability. This study explores the key factors for stable and efficient nitrogen removal in a one-stage partial nitritation/anammox (PNA) process with HAP-based granules.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Damian Hausherr, Robert Niederdorfer, Eberhard Morgenroth, Adriano Joss
Summary: The study demonstrates that anammox thrive under mainstream conditions and can withstand typical process disruptions, such as fluctuations in dissolved oxygen levels and temperature. Additionally, anammox activity showed good stability in response to changes in organic loading rate at bench scale and pilot scale experiments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Multidisciplinary
Yeonju Kim, Jaecheul Yu, Soyeon Jeong, Jeongmi Kim, Seongjae Park, Hyokwan Bae, Sung-Keun Rhee, Tatsuya Unno, Shou-Qing Ni, Taeho Lee
Summary: This study evaluated the effect of Ca2+ and Mg2+ augmentation on the recovery of freshwater anammox granules affected by salinity stress. The results showed that adding Ca2+ and Mg2+ at optimal concentrations increased the granules' activity and EPS production. Salinity stress did not significantly affect the microbial community in the granules. The alleviation effect of divalent cations on salinity stress might be associated with increased EPS production rather than changes to the anammox bacteria.
APPLIED SCIENCES-BASEL
(2022)
Article
Engineering, Environmental
Biru Zhou, Guangjiao Chen, Chifei Dong, Yushi Jiang, Haoyu Chen, Tong Ouyang, Yu-You Li, Yanlong Zhang
Summary: Fe(II) has concentration-dependent effects on specific anammox activity (SAA) and reactor performance, with low concentrations enhancing SAA and high concentrations inhibiting SAA. Long-term exposure to low Fe(II) concentrations can improve the tolerance of anammox to Fe(II). Fe(II) also affects extracellular polymeric substance (EPS) and the performance of anammox granules.
WATER ENVIRONMENT RESEARCH
(2021)
Review
Environmental Sciences
Pengcheng Wang, Bin Lu, Xiaoji Liu, Xiaoli Chai
Summary: Based on the reviews of the AnGS formation mechanism, micro-nuclei were found to be important in the granulation process of AnGS, and adding micro-nuclei directly to the reactor may be an efficient way to accelerate the formation of AnGS. This paper summarizes and discusses the use of inert particles, multivalent positive ions, and broken granule sludge as micro-nuclei to accelerate AnGS granulation. Possible research directions for accelerating AnGS formation by dosing micro-nuclei were also highlighted. The paper aims to provide a possible pathway for the rapid start-up of AnGS systems and references for the optimization and promotion of the AnGS process.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Hyungmin Choi, Jinsu Kim, Jihun Park, Changsoo Lee
Summary: Even small fluctuations in wastewater's organic matter significantly affect the structure and function of anammox microbial communities. Continuous organic load at 30 mg COD/L improved nitrogen removal efficiency without compromising anammox performance. Higher levels of continuous organic load caused inhibition of anammox activity, while transient organic load's inhibitory effect was fully reversed within one day. Loading an appropriately low amount of organic matter can improve the nitrogen removal performance of anammox granular sludge without sacrificing anammox activity and granule stability under mainstream conditions.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Andres Pichel, Andrea Fra, Nicolas Morales, Jose Luis Campos, Ramon Mendez, Anuska Mosquera-Corral, Angeles Val del Rio
Summary: This study demonstrates that despite the higher sCOD/N ratios in OFMSW digestate, good synergy can be achieved through AS pre-treatment in the PN-AMX process, resulting in better effluent quality and lower nitrogen removal rates.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Alba Pedrouso, Angeles Val del Rio, Nicolas Morales, Jose R. Vazquez-Padin, Jose Luis Campos, Anuska Mosquera-Corral
Summary: The study demonstrated that the anammox process can operate under municipal wastewater conditions without the need for a common acclimation period, shortening startup time. Nitrogen removal efficiency can be improved by increasing residual organic matter concentrations, and the anammox activity is significantly influenced by pH and temperature.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Agricultural Engineering
Alba Roibas-Rozas, Angeles Val del Rio, Almudena Hospido, Anuska Mosquera-Corral
Summary: By adding Na(HCO3) and adjusting the order of the settling stage, the accumulation of PHA was successfully increased, ultimately producing more PHA from wastewater.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Chemical
Alba Pedrouso, David Correa-Galeote, Paula Maza-Marquez, Belen Juarez-Jimenez, Jesus Gonzalez-Lopez, Belen Rodelas, Jose Luis Campos, Anuska Mosquera-Corral, Angeles Val del Rio
Summary: Partial nitritation was identified as a key step in autotrophic nitrogen removal processes at low temperatures. This study successfully maintained a nitritation process with simultaneous COD removal in a sequencing batch reactor operated at 15 degrees C. Despite fluctuations in municipal wastewater composition, a high nitrite accumulation ratio was observed, and the presence of nitrite oxidizing bacteria (NOB) was attributed to high levels of free nitrous acid (FNA) in the system.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ana T. Couto, Martim Cardador, Sergio Santorio, Luz Arregui, Benedetto Sicuro, Anuska Mosquera-Corral, Paula M. L. Castro, Catarina L. Amorim
Summary: This study isolated 15 strains of microalgae from sludge of a rainbow trout freshwater aquaculture farm, belonging to nine different genera of the Chlorophyta phylum. After culturing for 7 days in a raceway pond, these native microalgae isolates successfully removed approximately 99% of ammonium, 92% of nitrite, and 49% of nitrate from the effluent, with a biomass increase of around 17%.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Daniel Valenzuela-Heredia, Camila Panatt, Marisol Belmonte, Oscar Franchi, Dafne Crutchik, Jacques Dumais, Jose Ramon Vazquez-Padin, Yves Lesty, Alba Pedrouso, Angeles Val del Rio, Anuska Mosquera-Corral, Jose Luis Campos
Summary: A two-stage system was used to remove nitrogen from dewatering liquor of municipal WWTP sludge. Two strategies were tested to start up the system, demonstrating that dilution of THP/AD effluents is not required. Experimental results showed that inhibitory effects of organic compounds on the activity of ammonia oxidizing bacteria can be eliminated in the PN reactor.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Alba Roibas-Rozas, Montserrat Nunez, Anuska Mosquera-Corral, Almudena Hospido
Summary: Salinity is changing in aquatic systems due to human activities and climate change, and its effects on overall ecosystems are still not well understood. This research introduces a new framework to study the impacts of salinity variations, including the possible positive effects of emissions. The framework was validated by applying it to an estuarine area in northwestern Spain, where sharp drops in salt concentration have caused mass mortalities of shellfish.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Lucia Argiz, David Correa-Galeote, Angeles Val del Rio, Anuska Mosquera-Corral, Rebeca Gonzalez-Cabaleiro
Summary: It was found that the mode of oxygen, carbon, and nitrogen supply determines the enrichment of the culture in specific populations, thus influencing the type of intracellular compounds preferentially accumulated. Coupled carbon and nitrogen feeding regime mainly selects for TAG producers, while uncoupled feeding leads to PHA or TAG production depending on the rate of carbon supply under specific aeration rates and feast and famine phases lengths.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Annarita Sibilio, Clara Suner, Marcos Fernandez-Alfara, Judit Martin, Antonio Berenguer, Alexandre Calon, Veronica Chanes, Alba Millanes-Romero, Gonzalo Fernandez-Miranda, Eduard Batlle, Mercedes Fernandez, Raul Mendez
Summary: This study investigates the role of the RNA-binding protein CPEB4 in intestinal immune homeostasis and its contribution to the development of inflammatory bowel diseases and colorectal cancer. The researchers found that CPEB4 is expressed in the intestines after damage and is necessary for the translation of cytokine mRNA, such as interleukin-22. It plays a role in maintaining intestinal immune homeostasis, promoting repair and remodeling after tissue damage, and resolving inflammation. Chronic overexpression of CPEB4 is observed in inflammatory cells in patients with IBD and CRC, which promotes tumor development.
Article
Polymer Science
David Correa-Galeote, Lucia Argiz, Angeles Val del Rio, Anuska Mosquera-Corral, Belen Juarez-Jimenez, Jesus Gonzalez-Lopez, Belen Rodelas
Summary: The biosynthesis of PHAs from industrial wastes using MMCs enriched in PHA-accumulating bacteria is a promising alternative to petroleum-based plastics. This study investigated the shifts in bacterial communities in lab-scale reactors fed with fish-canning effluents, and found that a double growth limitation strategy effectively modulated the relative abundances of PHA-accumulating bacteria and increased PHA accumulation, regardless of saline conditions.
Article
Biology
Clara Suner, Annarita Sibilio, Judit Martin, Chiara Lara Castellazzi, Oscar Reina, Ivan Dotu, Adria Caballe, Elisa Rivas, Vittorio Calderone, Juana Diez, Angel R. Nebreda, Raul Mendez
Summary: Chronic inflammation is a major cause of disease. This study reveals the counterbalancing actions of RNA-binding proteins CPEB4 and TTP in inflammation resolution. CPEB4 stabilizes anti-inflammatory transcripts, while TTP directs mRNA deadenylation. The activities of CPEB4 and TTP are regulated through MAPK signaling. Maintaining the balance between these two opposing mechanisms is crucial for efficient inflammation resolution.
Editorial Material
Green & Sustainable Science & Technology
Jose Luis Campos, Anuska Mosquera-Corral, Angeles Val del Rio, Alba Pedrouso
Article
Biochemistry & Molecular Biology
Santo Fabio Corsino, Gaetano Di Bella, Francesco Traina, Lucia Argiz Montes, Angeles Val del Rio, Anuska Mosquera Corral, Michele Torregrossa, Gaspare Viviani
Summary: In this study, a biological strategy was employed in the MBR system to reduce membrane fouling tendency. By controlling the operating conditions, the organic matter was directed towards the accumulation of intracellular polymers (PHA), leading to a decrease in extracellular polymeric substances (EPS) and a reduction in fouling rate. This highlights the possibility of minimizing membrane fouling and recovering bioplastic precursors from wastewater in line with the circular economy model.
Article
Biotechnology & Applied Microbiology
Berta Duran-Arque, Manuel Canete, Chiara Lara Castellazzi, Anna Bartomeu, Anna Ferrer-Caelles, Oscar Reina, Adria Caballe, Marina Gay, Gianluca Arauz-Garofalo, Eulalia Belloc, Raul Mendez
Summary: This study systematically addresses the regulation and mechanisms of action of the vertebrate CPEB family. The results show that different members of the CPEB family regulate gene expression through different mechanisms and target mRNAs, leading to cellular adaptive responses.
Article
Green & Sustainable Science & Technology
Valentina Ortega, Andres Donoso-Bravo, Rolando Chamy-Maggy, Jose Luis Campos, Anuska Mosquera-Corral, Marisol Belmonte
Summary: This study aimed to evaluate the most significant factors affecting methane yield in olive alperujo during biomethane potential tests. The results showed that olive alperujo concentration was the most significant factor affecting methane yield.
