☆ 4.5 Article

Thioflavin derivatives as markers for amyloid-beta fibrils: Insights into structural features important for high-affinity binding

CHEMMEDCHEM (2008)

Journal

CHEMMEDCHEM

Volume 3, Issue 1, Pages 63-66

Publisher

WILEY-V C H VERLAG GMBH

DOI: 10.1002/cmdc.200700188

Keywords

-

Categories

Chemistry, Medicinal Pharmacology & Pharmacy

Ask authors/readers for more resources

Protocol

Community support

Reagent

Community support

Authors

I am an author on this paper

Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5

Not enough ratings

Secondary Ratings

Novelty

-

Significance

-

Scientific rigor

-

Rate this paper

Recommended

Article Multidisciplinary Sciences

In situ kinetic measurements of α-synuclein aggregation reveal large population of short-lived oligomers

Enrico Zurlo, Pravin Kumar, Georg Meisl, Alexander J. Dear, Dipro Mondal, Mireille M. A. E. Claessens, Tuomas P. J. Knowles, Martina Huber

Summary: Understanding the mechanism of amyloid protein assembly is crucial for studying neurodegenerative diseases. EPR method can directly measure the amount of intermediates during the aggregation process without the need for separation, providing insights into the kinetics of intermediate species formation and conversion. The study on alpha-synuclein aggregation using EPR revealed a large population of short-lived primary oligomers formed directly from monomeric species.

PLOS ONE (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Experimental Insights into Conformational Ensembles of Assembled β-Sheet Peptides

Lanlan Yu, Ruonan Wang, Shucong Li, Ufuoma I. Kara, Eric C. Boerner, Boyuan Chen, Feiyi Zhang, Zhongyi Jian, Shuyuan Li, Mingwei Liu, Yang Wang, Shuli Liu, Yanlian Yang, Chen Wang, Wenbo Zhang, Yuxing Yao, Xiaoguang Wang, Chenxuan Wang

Summary: Scanning tunneling microscopy (STM) is used to distinguish 18 coexisting conformational substates of the β-strand of hIAPP 8-37 and analyze the peptide-peptide interactions. This method also validates the existence of multiple conformations in other β-sheet peptide assemblies. The results provide insights into the conformational ensemble and interpeptide interactions in β-sheet peptide assembly.

ACS CENTRAL SCIENCE (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Destabilization of Alzheimer's Aβ42 protofibrils with acyclovir, carmustine, curcumin, and tetracycline: insights from molecular dynamics simulations

Ishrat Jahan, Shahid M. Nayeem

Summary: Alzheimer's disease (AD) is a common neurodegenerative disease characterized by the deposition and aggregation of amyloid-beta-peptides in neural tissue. Drugs like acyclovir, carmustine, curcumin, and tetracycline have been suggested as potential molecules for destabilizing A beta protofibrils. These drugs bind to the hydrophobic grooves of A beta protofibrils, destabilizing the beta-strand structure and inhibiting A beta aggregations. Tetracycline is found to be the most effective among these drugs in diminishing A beta aggregations.

NEW JOURNAL OF CHEMISTRY (2021)

Add to Collection

Article Chemistry, Physical

Evidence of enhanced inhibitory potential of Ferulic Acid Ethyl Ester towards modulating fibrillation and disassembling insulin fibrils

Sadia Malik, Faisal Nabi, Mohammad Khursheed Siddiqi, Aiman Masroor, Malik Hisamuddin, Tajalli Ilm Chandel, Nabeela Majid, Rizwan Hasan Khan

Summary: This study confirms the inhibitory effect of lipophilic FAEE on insulin fibrillation, which can cross the blood brain barrier and stabilize the monomeric state of insulin, thereby preventing the extension of fibrillation.

JOURNAL OF MOLECULAR LIQUIDS (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Ganglioside GM1 produces stable, short, and cytotoxic Aβ40 protofibrils

Manjeet Kumar, Magdalena I. Ivanova, Ayyalusamy Ramamoorthy

Summary: In patients with early pathological changes of Alzheimer's disease, Monosialoganglioside GM1-bound amyloid beta-peptides are detected. This study reveals that non-micellar GM1 can modulate A beta(40) aggregation, resulting in the formation of stable, short, rod-like, and cytotoxic A beta(40) protofibrils that can enhance the aggregation of both A beta(40) and A beta(42).

CHEMICAL COMMUNICATIONS (2023)

Add to Collection

Article Chemistry, Physical

Silver nanoparticles as inhibitors of insulin amyloid formation: A fluorescence study

Kateryna Vus, Uliana Tarabara, Ihor Danylenko, Yaroslav Pirko, Tetiana Krupodorova, Alla Yemets, Yaroslav Blume, Vitalii Turchenko, Dmytro Klymchuk, Petro Smertenko, Olga Zhytniakivska, Valeriya Trusova, Sergii Petrushenko, Sergey Bogatyrenko, Galyna Gorbenko

Summary: Silver nanoparticles (SNPs) synthesized extracellularly using extracts of different genus Pleurotus fungi samples showed pronounced anti-amyloid effect in vitro, suggesting their potential application as effective anti-amyloid agents.

JOURNAL OF MOLECULAR LIQUIDS (2021)

Add to Collection

Article Chemistry, Physical

Polyethylene-glycol-modified zwitterionic polymer assisted protein aggregation arrest and refolding

Alisha Debas, Kazuaki Matsumura, Robin Rajan

Summary: This study demonstrated that the modified zwitterionic polymer, poly-sulfobetaine (p-SPB), in combination with polyethylene glycol (PEG), effectively suppressed heat-induced lysozyme aggregation. The modified polymers prevented the formation of amyloid fibrils and retained the enzymatic activity of lysozyme. Additionally, these polymers facilitated the refolding of the protein and regained the lost activity.

MOLECULAR SYSTEMS DESIGN & ENGINEERING (2022)

Add to Collection

Article Multidisciplinary Sciences

Enhanced optical asymmetry in supramolecular chiroplasmonic assemblies with long-range order

Jun Lu, Yao Xue, Kalil Bernardino, Ning-Ning Zhang, Weverson R. Gomes, Naomi S. Ramesar, Shuhan Liu, Zheng Hu, Tianmeng Sun, Andre Farias de Moura, Nicholas A. Kotov, Kun Liu

Summary: The study demonstrates that the limitations of optical asymmetry in chiral assemblies of plasmonic nanoparticles can be overcome by long-range organization of nanoparticles, similar to liquid crystals. This long-range order in helical assemblies enhances optical asymmetry by reducing scattering and activating energy states with antiparallel orientation of dipoles.

SCIENCE (2021)

Add to Collection

Article Chemistry, Applied

Fluorescence photoswitch of stiff-stilbene derivatives for anti-counterfeiting

Guoxing Liu, Juhua Leng, Qingyang Zhou, Zhe Deng, Linlin Shi, Cailing Fan, Xiufang Xu, Mao-Ping Song

Summary: This study focused on the precise improvement of the photo-isomerized reaction of molecular photoswitches and its importance in the fabrication of high-performance smart materials. The study synthesized a series of stiff-stilbene derivatives and investigated their photoisomerization properties, revealing the influence of electronic effects on the photoisomerization properties. The study also demonstrated the application of these molecular photoswitches in fluorescence behavior and high-secrecy-level anti-counterfeiting.

DYES AND PIGMENTS (2022)

Add to Collection

Article Chemistry, Physical

How oxidized EGCG remodels α-synuclein fibrils into non-toxic aggregates: insights from computational simulations

Priscila Baltazar Goncalves, Fernando L. Palhano, Yraima Cordeiro, Ana Carolina Renno Sodero

Summary: The misfolding and aggregation of α-synuclein is a pathological hallmark of Parkinson's disease. Epigallocatechin-3-gallate (EGCG) can prevent the formation of toxic aggregates and remodel existing toxic fibrils into non-toxic aggregates. Through molecular docking and dynamics simulations, the study investigates the interactions of oxidized EGCG and Thioflavin T with mature α-syn fibrils.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2023)

Add to Collection

Article Chemistry, Physical

Benzanthrone dyes as mediators of cascade energy transfer in insulin amyloid fibrils

Uliana Tarabara, Elena Kirilova, Georgiy Kirilov, Kateryna Vus, Olga Zhytniakivska, Valeriya Trusova, Galyna Gorbenko

Summary: Amyloid fibrils can serve as a molecular framework for multi-step Forster resonance energy transfer (msFRET). Novel benzanthrone dyes are being explored as potential mediators of msFRET in insulin amyloid fibrils. The study aims to evaluate the effectiveness of these dyes in detecting amyloid fibrils.

JOURNAL OF MOLECULAR LIQUIDS (2021)

Add to Collection

Article Chemistry, Physical

Functionalization of insulin nanofibrils with fluorophores involved in cascade Forster resonance energy transfer

Galyna Gorbenko, Uliana Tarabara, Olga Zhytniakivska, Kateryna Vus, Valeriya Trusova

Summary: Amyloid protein nanofibrils have attracted considerable attention in biomedical and nanotechnological aspects. This study evaluates the functionalization of insulin nanofibrils with styryl pyridinium dyes (SPD), thioflavin T (ThT), and squaraine dyes. The results show that the SPD- ThT - SPD - SQ4 - SQ1 cascade provides efficient energy transfer in fibrillar protein compared to non-fibrillized protein. The spectral overlap between ThT and SPD enhances the relay fluorophores' effectiveness in the FRET process. The quantitative analysis reveals that the insulin fibril volume is accessible to fluorophores in different spatial distributions.

MOLECULAR SYSTEMS DESIGN & ENGINEERING (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Atomic Resolution Insights into pH Shift Induced Deprotonation Events in LS-Shaped Aβ(1-42) Amyloid Fibrils

Nina Becker, Benedikt Frieg, Lothar Gremer, Tatsiana Kupreichyk, Luis Gardon, Patrick Freiburg, Philipp Neudecker, Dieter Willbold, Holger Gohlke, Henrike Heise

Summary: Alzheimer's disease is characterized by the accumulation of misfolded amyloid-fi protein (Afi). We found that Afi(1-42) fibrils with a predominant fold can be obtained at acidic pH, and their structure differs from polymorphs obtained at neutral pH.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Atomic Resolution Insights into pH Shift Induced Deprotonation Events in LS-Shaped Aβ(1-42) Amyloid Fibrils

Nina Becker, Benedikt Frieg, Lothar Gremer, Tatsiana Kupreichyk, Luis Gardon, Patrick Freiburg, Philipp Neudecker, Dieter Willbold, Holger Gohlke, Henrike Heise

Summary: Alzheimer's disease is associated with the deposition of misfolded aggregates of the amyloid-fi protein (Afi). The study found that relatively homogeneous Afi(1-42) fibrils can be obtained at acidic pH, and the stability and structure of these fibrils are affected by a pH shift. Through various techniques, it was demonstrated that the LS-fold of the Afi(1-42) fibrils remains stable at physiological pH values, with the N-terminus being part of the fibril core.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2023)

Add to Collection

Article Biochemistry & Molecular Biology

New Evidence on a Distinction between Aβ40 and Aβ42 Amyloids: Thioflavin T Binding Modes, Clustering Tendency, Degradation Resistance, and Cross-Seeding

Anna Sulatskaya, Georgy N. Rychkov, Maksim Sulatsky, Ekaterina Mikhailova, Nadezhda M. Melnikova, Veronika S. Andozhskaya, Irina M. Kuznetsova, Konstantin K. Turoverov

Summary: This comprehensive study provides new evidence on the amyloid polymorphism of Aβ 40 and Aβ 42. It shows that Aβ 42 fibers have a higher tendency to form large clots and exhibit stronger resistance to destabilizing effects, as well as explicit cytotoxicity. In contrast, Aβ 40 fibers have a lower tendency to cluster and weaker resistance to external factors. Additionally, cross-seeding of Aβ 40 fibrillogenesis using preformed Aβ 42 fibrils changes the morphology and increases the stability and cytotoxicity of Aβ 40 fibrils.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Add to Collection

Article Chemistry, Multidisciplinary

The Glutathione/Metallothionein System Challenges the Design of Efficient O2-Activating Copper Complexes

Alice Santoro, Jenifer S. Calvo, Manuel David Peris-Diaz, Artur Krezel, Gabriele Meloni, Peter Faller

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Add to Collection

Article Chemistry, Organic

Straightforward convergent access to 2-arylated polysubstituted benzothiazoles

Omar Sadek, David M. Perrin, Emmanuel Gras

TETRAHEDRON (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Phosphine-phosphonium ylides as ligands in palladium-catalysed C2-H arylation of benzoxazoles

Zhenyu Yao, Xing Lin, Remi Chauvin, Lianhui Wang, Emmanuel Gras, Xiuling Cui

CHINESE CHEMICAL LETTERS (2020)

Add to Collection

Article Immunology

Phthiocerol Dimycocerosates FromMycobacterium tuberculosisIncrease the Membrane Activity of Bacterial Effectors and Host Receptors

Jacques Augenstreich, Evert Haanappel, Fadel Sayes, Roxane Simeone, Valerie Guillet, Serge Mazeres, Christian Chalut, Lionel Mourey, Roland Brosch, Christophe Guilhot, Catherine Astarie-Dequeker

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Copper-binding motifs Xxx-His or Xxx-Zzz-His (ATCUN) linked to an antimicrobial peptide: Cu-binding, antimicrobial activity and ROS production

Merwan Bouraguba, Elise Glattard, Maxime Naude, Remi Pelletier, Christopher Aisenbrey, Burkhard Bechinger, Laurent Raibaut, Vincent Lebrun, Peter Faller

JOURNAL OF INORGANIC BIOCHEMISTRY (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Reproducibility Problems of Amyloid-β Self-Assembly and How to Deal With Them

Peter Faller, Christelle Hureau

Summary: Controlling protein and peptide aggregation in vitro and obtaining reproducible results poses a challenge, which is scarcely reported and discussed in the literature, potentially hindering progress in the field and misleading newcomers.

FRONTIERS IN CHEMISTRY (2021)

Add to Collection

Review Chemistry, Multidisciplinary

Amyloid Oligomers: A Joint Experimental/Computational Perspective on Alzheimer's Disease, Parkinson's Disease, Type II Diabetes, and Amyotrophic Lateral Sclerosis

Phuong H. Nguyen, Ayyalusamy Ramamoorthy, Bikash R. Sahoo, Jie Zheng, Peter Faller, John E. Straub, Laura Dominguez, Joan-Emma Shea, Nikolay Dokholyan, Alfonso De Simone, Buyong Ma, Ruth Nussinov, Saeed Najafi, Son Tung Ngo, Antoine Loquet, Mara Chiricotto, Pritam Ganguly, James McCarty, Mai Suan Li, Carol Hall, Yiming Wang, Yifat Miller, Simone Melchionna, Birgit Habenstein, Stepan Timr, Jiaxing Chen, Brianna Hnath, Birgit Strodel, Rakez Kayed, Sylvain Lesne, Guanghong Wei, Fabio Sterpone, Andrew J. Doig, Philippe Derreumaux

Summary: Protein misfolding and aggregation are common in amyloidogenic diseases, and understanding how amyloid species become toxic is crucial for treatment development. Experiments using computer modeling, in vitro and in vivo studies, as well as pharmacology, have provided valuable insights into the accumulation and deposition of amyloid species, contributing to research on diseases like Alzheimer's, Parkinson's, diabetes, and ALS.

CHEMICAL REVIEWS (2021)

Add to Collection

Review Chemistry, Inorganic & Nuclear

Extracellular Cu2+ pools and their detection: From current knowledge to next-generation probes

Enrico Falcone, Michael Okafor, Nicolas Vitale, Laurent Raibaut, Angelique Sour, Peter Faller

Summary: The study focuses on sensors of the Cu2+ state and discusses the challenges in detecting labile and thermodynamically accessible Cu2+ using luminescent or MRI-active probes in extracellular fluids. The specificity, affinity for Cu2+, and competition with other ligands like Zn2+ play crucial roles in designing potential sensors for measuring Cu2+ accurately. However, current research shows that developing sensors capable of specifically detecting Cu2+ in biological systems remains a significant challenge.

COORDINATION CHEMISTRY REVIEWS (2021)

Add to Collection

Article Chemistry, Organic

Chiral Benzothiazole Monofluoroborate Featuring Chiroptical and Oxygen-Sensitizing Properties: Synthesis and Photophysical Studies

Omar Sadek, Laura Abad Galan, Frederic Gendron, Bruno Baguenard, Stephan Guy, Amina Bensalah-Ledoux, Boris Le Guennic, Olivier Maury, David M. Perrin, Emmanuel Gras

Summary: This study presents the design of a single molecule with potential for different diagnostic modes and the ability to sensitize oxygen for photodynamic therapy. Through synthesis and chiral resolution, the absolute configuration of enantiomeric chiral monofluoroborates was determined. Experimental and theoretical studies confirmed the compound's oxygen sensitization ability even without heavy atoms, paving the way for multimodal diagnostic tools and potential therapeutic applications.

JOURNAL OF ORGANIC CHEMISTRY (2021)

Add to Collection

Article Biochemistry & Molecular Biology

Mycobacteria-host interactions in human bronchiolar airway organoids

Nino Iakobachvili, Stephen Adonai Leon-Icaza, Kevin Knoops, Norman Sachs, Serge Mazeres, Roxane Simeone, Antonio Peixoto, Celia Bernard, Marlene Murris-Espin, Julien Mazieres, Kaymeuang Cam, Christian Chalut, Christophe Guilhot, Carmen Lopez-Iglesias, Raimond B. G. Ravelli, Olivier Neyrolles, Etienne Meunier, Geanncarlo Lugo-Villarino, Hans Clevers, Celine Cougoule, Peter J. Peters

Summary: The study found that Mycobacterium tuberculosis and Mycobacterium abscessus mainly exist as extracellular bacteria in human airway organoids, infecting epithelial cells with low efficiency. The organoids respond to infection by modulating cytokine, antimicrobial peptide, and mucin gene expression. Adult stem cell-derived airway organoids can be used to elucidate early events of mycobacterial infection in human settings.

MOLECULAR MICROBIOLOGY (2022)

Add to Collection

Article Microbiology

Rv0180c contributes to Mycobacterium tuberculosis cell shape and to infectivity in mice and macrophages

Delphine Payros, Henar Alonso, Wladimir Malaga, Arnaud Volle, Serge Mazeres, Sebastien Dejean, Sophie Valiere, Flavie Moreau, Stephanie Balor, Alexandre Stella, Lucie Combes-Soia, Odile Burlet-Schiltz, Olivier Bouchez, Jerome Nigou, Catherine Astarie-Dequeker, Christophe Guilhot

Summary: This study identified new bacterial genes using a genome-wide approach and revealed their importance in the early interaction of M. tuberculosis with the host, particularly the association with the CR3 receptor. Additionally, the gene rv0180c was found to play a role in the bacterial cell envelope, which could affect the way M. tuberculosis invades host cells and provides a new perspective on modulating bacterial infectivity.

PLOS PATHOGENS (2021)

Add to Collection

Article Biochemistry & Molecular Biology

N-Heterocyclic Carbene-Iridium Complexes as Photosensitizers for In Vitro Photodynamic Therapy to Trigger Non-Apoptotic Cell Death in Cancer Cells

Xing Wang, Chen Zhang, Ryma Madji, Camille Voros, Serge Mazeres, Christian Bijani, Celine Deraeve, Olivier Cuvillier, Heinz Gornitzka, Marie-Lise Maddelein, Catherine Hemmert

Summary: A series of novel iridium complexes were synthesized and characterized as potential photosensitizers for photodynamic therapy (PDT) applications. Four complexes showed strong selectivity against cancer cells with a selectivity index (SI) ranging from 8 to 34 compared to non-cancerous cells. The best selective complex, 4a, localized in mitochondria and induced a non-apoptotic cell death.

MOLECULES (2023)

Add to Collection

Article Multidisciplinary Sciences

Nanoscale clustering of mycobacterial ligands and DC-SIGN host receptors are key determinants for pathogen recognition

Albertus Viljoen, Alain Vercellone, Myriam Chimen, Gerald Gaibelet, Serge Mazeres, Jerome Nigou, Yves F. Dufrene

Summary: The pathogenic bacterium Mycobacterium tuberculosis evades the immune system by binding to the C-type lectin DC-SIGN on dendritic cells. Our multidisciplinary study combining atomic force microscopy, Forster resonance energy transfer, and bioassays unravels the molecular mechanism behind the selective recognition of this receptor. We found that the distribution of DC-SIGN ligands differs between MyMTBC species, with dense nanodomains observed on M. bovis BCG. Upon bacteria-host cell adhesion, ligand nanodomains induce the recruitment and clustering of DC-SIGN, highlighting the importance of ligand clustering in pathogen recognition.

SCIENCE ADVANCES (2023)

Add to Collection

Article Chemistry, Multidisciplinary

How trimerization of CTR1 N-terminal model peptides tunes Cu-binding and redox-chemistry

Thibaut Galler, Vincent Lebrun, Laurent Raibaut, Peter Faller, Nina E. Wezynfeld

CHEMICAL COMMUNICATIONS (2020)

Add to Collection

Article Chemistry, Multidisciplinary

A terbium(iii) luminescent ATCUN-based peptide sensor for selective and reversible detection of copper(ii) in biological media

Enrico Falcone, Paulina Gonzalez, Lucie Lorusso, Olivier Seneque, Peter Faller, Laurent Raibaut

CHEMICAL COMMUNICATIONS (2020)

Add to Collection

No Data Available

© Peeref 2019-2024. All rights reserved.