Article
Chemistry, Multidisciplinary
Yi Yan, Xiao-Yu Liu, An Lu, Xiang-Yu Wang, Lin-Xia Jiang, Jian-Cheng Wang
Summary: RNA-based therapy is a promising strategy for disease treatment, and recent advances in non-viral delivery systems have shown potential in protecting RNA, facilitating cell internalization, and controlled release of therapeutics.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Materials Science, Multidisciplinary
Jing Chen, Wen Nie, Yuzhu Hu, Yangmei Shen, Yunzhu Lin, Bilan Wang, Zhiyong Qian, Xiang Gao
Summary: Drug resistance is a challenging problem in cancer chemotherapy. The combination of molecular targeted therapy and traditional chemotherapy shows potential clinical benefits. A folic acid-modified targeted drug/gene delivery system was developed to co-deliver Paclitaxel (PTX) and plasmid shBCL-2, which can enhance the sensitivity of cancer cells to chemotherapy drugs and improve therapeutic effect.
APPLIED MATERIALS TODAY
(2022)
Article
Chemistry, Inorganic & Nuclear
Junfeng Kou, Jinchao Shen, Mingwei Lin, Kai Xiong, Lili Wang, Fangmian Wei, Junfeng Zhang
Summary: Four novel photosensitizers of nitrogen-heterocyclic ruthenium polypyridyl complexes were reported, and their photodynamic activities were studied on 2D and 3D HeLa cancer models. These complexes showed high singlet oxygen quantum yields and TPA cross-sections, as well as high phototoxicity against 2D HeLa tumor cells. Flow cytometry analysis revealed that these complexes induced cell death mainly through apoptosis upon singlet oxygen-dependent reaction.
DALTON TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Raphael T. Ryan, Austin C. Hachey, Kimberly Stevens, Sean R. Parkin, Richard J. Mitchell, John P. Selegue, David K. Heidary, Edith C. Glazer
Summary: A systematic study was conducted to alter metal-ligand bond lengths, angles, and intraligand distortion by changing the chelate ring size and introducing a linker atom or group. This study lays the foundation for creating photoactive systems using alternative types of distortion to enhance photosubstitution reactions.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Hui Zu, Danchen Gao
Summary: Although non-viral vectors in gene therapy have low cytotoxicity and immunogenicity, they still face challenges in gene transfer efficiency, specificity, gene expression duration, and safety. Overcoming these obstacles is necessary to enhance the effectiveness of non-viral vectors in gene therapy.
Review
Engineering, Biomedical
Jiayu Gu, Zhourui Xu, Qiqi Liu, Shiqi Tang, Wenguang Zhang, Shouxia Xie, Xiaoyan Chen, Jiajie Chen, Ken-Tye Yong, Chengbin Yang, Gaixia Xu
Summary: In recent years, messenger RNA (mRNA) vaccines have shown great potential as replacements for conventional vaccines due to their low risk of mutagenesis, safety and effectiveness, rapid and scalable production, and low-cost manufacturing. Non-viral mRNA vectors are considered ideal choices for future clinical translation due to their safety, sufficient packaging capability, low immunogenicity, and versatility. This review focuses on the recent progress in the development of non-viral mRNA vectors, including lipid nanoparticles, polymers, peptides, and exosomes. It also discusses the latest advances in clinical trials of mRNA vaccines, as well as the current challenges and future possibilities for the clinical translation of these vectors.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Organic
Michela Lomazzi, Valentina Franceschi, Valentina Bagnacani, Carlo Alberto Vezzoni, Gaetano Donofrio, Alessandro Casnati, Francesco Sansone
Summary: The tetra-L-arginino-tetrahexyloxycalix[4]arene 1 has shown remarkable abilities to compact and deliver various types of nucleic acid cargos into cells. Small structural changes in the calixarene vectors may affect their ability to compact DNA and deliver cargo into cells.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Ilona Uzieliene, Ursule Kalvaityte, Eiva Bernotiene, Ali Mobasheri
Summary: Gene therapy has been explored as a potential treatment for osteoarthritis, with a focus on non-viral gene delivery technologies. While viral gene delivery has been more popular due to higher efficiency, efforts to enhance transfection efficiency of non-viral methods have shown promise for OA research and clinical applications. More research is needed to optimize transfection efficiency and duration of gene expression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Chenfei Wang, Chaolan Pan, Haiyang Yong, Feifei Wang, Tao Bo, Yitong Zhao, Bin Ma, Wei He, Ming Li
Summary: Gene therapy holds great promise for treating a wide range of genetic diseases by delivering functional genes into targeted cells or tissues. However, the lack of safe and efficient gene delivery vehicles remains a major obstacle to its clinical implementation. This review comprehensively outlines the novel non-viral gene delivery vectors with potential applications in gene therapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Yu Wang, Yan Tang, Xiao-mei Zhao, Gui Huang, Jin-hong Gong, Shu-di Yang, Hui Li, Wen-jun Wan, Chang-hao Jia, Gang Chen, Xue-nong Zhang
Summary: In this study, a multifunctional non-viral vector was constructed for efficient delivery of CRISPR/Cas9 plasmid. The vector actively targeted tumor cells and delivered the cargo into cell nuclei, resulting in genome editing effects. By disrupting the MTH1 gene, the growth of non-small cell lung cancer was inhibited, promoting cell apoptosis in tumor tissue and reducing liver metastasis.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Adriana Aurelia Chis, Carmen Maximiliana Dobrea, Luca-Liviu Rus, Adina Frum, Claudiu Morgovan, Anca Butuca, Maria Totan, Anca Maria Juncan, Felicia Gabriela Gligor, Anca Maria Arseniu
Summary: Gene-directed enzyme prodrug therapy (GDEPT) is a promising strategy for prodrug delivery, with benefits including enhanced efficacy and reduced off-target toxicity. Non-viral vectors, such as dendrimers, have gained interest due to their decreased immunogenicity and high specificity for delivering genes in GDEPT therapy.
Review
Chemistry, Multidisciplinary
Shuai Qu, Renfa Liu, Nisi Zhang, Yunxue Xu, Xiuli Yue, Zhifei Dai
Summary: This article reviews the recent advances in targeted non-viral nucleic acid delivery for gene therapy of atherosclerosis, including hepatocyte-targeted delivery and delivery to different cells within the plaques.
Article
Chemistry, Inorganic & Nuclear
Robin Vinck, Albert Gandioso, Pierre Burckel, Bruno Saubamea, Kevin Cariou, Gilles Gasser
Summary: Four new ruthenium(II) polypyridyl complexes were synthesized and their properties were studied. It was found that these complexes could self-assemble into nanoparticles and accumulate in lysosomes upon internalization by cells. Conjugation with biotin increased cellular uptake but did not increase phototoxicity. However, these complexes showed high phototoxicity upon irradiation at long wavelengths (645-670 nm) and self-assembling behavior, making them promising for the development of new lysosome-targeted photosensitizers for photodynamic therapy.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Physical
Prashant Kumar, Falguni Chandra, Paltan Laha, P. Kavyashree, Srikanta Patra, Apurba Lal Koner
Summary: This study reports how DNA binding of ruthenium polypyridyl complexes enhances their efficiency as photosensitizers, generating reactive singlet oxygen to cause DNA damage and kill cancer cells, while also inhibiting bacterial growth. Differential localization of the complexes in cancer cell nuclei and near the nuclear membrane of non-cancer cells provide a potential platform for novel antibiotic discovery and cancer therapy.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Review
Biochemistry & Molecular Biology
Darya A. Savenkova, Aelita-Luiza A. Makarova, Igor K. Shalik, Dmitry V. Yudkin
Summary: Gene therapy is commonly used for treating incurable disorders, often using viral vectors to deliver genetic material. However, viral vectors have disadvantages such as immune response and disruption of cell metabolism, which require evaluation of side effects and safety. Many gene therapy techniques use non-coding RNA to regulate gene expression, but this can affect cellular miRNA pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Satish S. Bhat, Vidyanand K. Revankar, Rahul V. Pinjari, S. Naveen, N. K. Lokanath, Vijay Kumbar, Kishore Bhat, Dhoolesh G. Kokare
NEW JOURNAL OF CHEMISTRY
(2018)
Article
Crystallography
Vitthal A. Kawade, Satish S. Bhat, Ray J. Butcher
JOURNAL OF CHEMICAL CRYSTALLOGRAPHY
(2019)
Article
Chemistry, Multidisciplinary
Satish Shantaram Bhat, Naveen Shivalingegowda, Vidyanand Krishna Revankar, Vitthal Ajinath Kawade, Ray J. Butcher, Neratur Krishnappagowda Lokanath
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Satish S. Bhat, Avinash S. Kumbhar, Neeraja Purandare, Ayesha Khan, Gunter Grampp, Peter Loennecke, Evamarie Hey-Hawkins, Ruchi Dixit, Kumar Vanka
JOURNAL OF INORGANIC BIOCHEMISTRY
(2020)
Article
Chemistry, Inorganic & Nuclear
Ganesh S. Hegde, Satish S. Bhat, Sandeep P. Netalkar, Pooja L. Hegde, Avinash Kotian, Ray J. Butcher, Vidyanand K. Revankar
Summary: A series of ligand 3,5-di-tert-butyl-2-hydroxy-N?-((2-oxo-1,2-dihydroquinolin-3-yl)methylene)benzohydrazide and its metal complexes were synthesized and characterized using various techniques. The copper(II) complex showed the best antimicrobial activity, particularly against gram-positive S. Aureus.
INORGANICA CHIMICA ACTA
(2021)
Article
Crystallography
Mukta B. Baraskar, Vitthal A. Kawade, Satish S. Bhat, Ray J. Butcher, Manali Mohite, Nikita Deshmukh, Anupa A. Kumbhar
Summary: This article reports a one-dimensional coordination polymer that forms a three-dimensional network through hydrogen-bonding, pi-pi stacking, and ion-dipole interactions. The polymer shows thermal stability up to 166 degrees C and is capable of cleaving plasmid DNA through an oxidative mechanism involving hydroxyl and superoxide radicals at low concentrations.
JOURNAL OF CHEMICAL CRYSTALLOGRAPHY
(2022)
Article
Chemistry, Inorganic & Nuclear
Pooja Lokesh Hegde, Krishna Naik, Satish S. Bhat, Sabiha A. Shaikh, Ray J. Butcher, S. Naveen, N. K. Lokanath, Vidyanand K. Revankar
Summary: A ligand HL and its cobalt(III), nickel(II) and copper(II) complexes were synthesized and structurally characterized. Complex 3 showed better antitubercular activity compared to standard drug, and exhibited higher cytotoxicity in HEK293 cell lines. Complexation increased the inhibition of tuberculosis bacteria compared to free ligand.
JOURNAL OF COORDINATION CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sabiha A. Shaikh, Satish S. Bhat, Vidyanand K. Revankar, Badarinath D. Kulkarni, Karthik Kumara, N. K. Lokanath, Vijay Kumbar, Kishore Bhat, Ray J. Butcher
Summary: Two fluorophore tagged copper(II) complexes have been synthesized and structurally characterized, showing high plasmid DNA cleavage activity and better cytotoxicity against A549 and MCF-7 cell lines, inducing an apoptotic mode of cell death.
Article
Chemistry, Physical
Pooja Lokesh Hegde, Satish S. Bhat, Vidyanand K. Revankar, Sabiha A. Shaikh, Karthik Kumara, N. K. Lokanath
Summary: In this study, three novel copper (II) complexes containing a thiosemicarbazone ligand were synthesized and characterized. The complexes were characterized using various spectroscopic techniques and the crystal structure of one of the complexes was confirmed using single-crystal X-ray analysis. In vitro experiments showed that these copper (II) complexes exhibited excellent anti-TB activity at nano-molar concentrations and low cytotoxicity.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Chemistry, Multidisciplinary
Satish Shantaram Bhat, Vitthal A. Kawade, Vidyanand K. Revankar, Vijay Kumbar, Kishore Bhat
Summary: In this study, a novel mixed-ligand fluorescent copper(II) complex was synthesized and characterized. The complex exhibited a distorted square pyramidal geometry and high fluorescence properties. It also showed promising anticancer activity.
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Sabiha A. Shaikh, Satish S. Bhat, Vidyanand K. Revankar, S. Naveen, N. K. Mahesha, N. K. Loknath, Vijay Kumbar
Summary: Mononuclear later first-row transition metal complexes incorporating 1,8-naphthalimide based ligand were designed and their photophysical properties and antibacterial activities were studied. Ni(II) and Cu(II) complexes exhibited good activity against certain bacterial strains.
JOURNAL OF COORDINATION CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sabiha A. Shaikh, Satish S. Bhat, Vidyanand K. Revankar, S. Naveen, Karthik Kumara, N. K. Lokanath, Ray J. Butcher, Vijay Kumbar, Kishore Bhat
Summary: A novel 1,8-naphthalimide-containing ligand and its three mononuclear complexes were synthesized and characterized. The ligand exhibited a non-planar structure with π···π stacking interaction, while the complexes showed a seesaw geometry. The complexes exhibited DNA binding in an intercalative mode and demonstrated improved cytotoxicity towards cancer cells.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sabiha A. Shaikh, Satish S. Bhat, Pooja L. Hegde, Vidyanand K. Revankar, Anup Kate, Deepti Kirtani, Anupa A. Kumbhar, Vijay Kumbar, Kishore Bhat
Summary: The two novel fluorophore labelled copper(II) complexes exhibit high fluorescence, efficient DNA cleavage, interactions with proteins, and significant anticancer activity, inducing apoptotic cell death.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Satish S. Bhat, S. Naveen, Vidyanand K. Revankar, N. K. Lokanath, Rahul V. Pinjari, Vijay Kumbar, Kishore Bhat
NEW JOURNAL OF CHEMISTRY
(2020)
Article
Chemistry, Inorganic & Nuclear
Mainak Mitra, Olaf Cusso, Satish S. Bhat, Mingzhe Sun, Marco Cianfanelli, Miquel Costas, Ebbe Nordlander
DALTON TRANSACTIONS
(2019)
