Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 18, Issue 3, Pages 869-879Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201102509
Keywords
DNA structures; helical structures; NMR spectroscopy; nucleic acids; orthogonal nucleic acids; synthetic biology
Categories
Funding
- K. U. Leuven
- DFG
Ask authors/readers for more resources
Orthogonal nucleic acids are chemically modified nucleic acid polymers that are unable to transfer information with natural nucleic acids and thus can be used in synthetic biology to store and transfer genetic information independently. Recently, it was proposed that xylose-DNA (dXNA) can be considered to be a potential candidate for an orthogonal system. Herein, we present the structure in solution and conformational analysis of two self-complementary, fully modified dXNA oligonucleotides, as determined by CD and NMR spectroscopy. These studies are the initial experimental proof of the structural orthogonality of dXNAs. In aqueous solution, dXNA duplexes predominantly form a linear ladderlike (type-1) structure. This is the first example of a furanose nucleic acid that adopts a ladderlike structure. In the presence of salt, an equilibrium exists between two types of duplex form. The corresponding nucleoside triphosphates (dXNTPs) were synthesized and evaluated for their ability to be incorporated into a growing DNA chain by using several natural and mutant DNA polymerases. Despite the structural orthogonality of dXNA, DNA polymerase beta mutant is able to incorporate the dXNTPs, showing DNA-dependent dXNA polymerase activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available