Journal
CHEMISTRY LETTERS
Volume 40, Issue 2, Pages 206-208Publisher
CHEMICAL SOC JAPAN
DOI: 10.1246/cl.2011.206
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Funding
- Center for Practical Chemical Wisdom
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [22580093]
- Grants-in-Aid for Scientific Research [22580093] Funding Source: KAKEN
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A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from,n-AP through an enzymatic Kolbe-Schmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70 mM PAS was produced at 30 degrees C in 15h with a conversion yield of 70% (mol/mol).
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