Article
Biochemistry & Molecular Biology
Jia Yu, Barbara Leibiger, Shao-Nian Yang, Stephen B. B. Shears, Ingo B. B. Leibiger, Per-Olof Berggren, Christopher J. J. Barker
Summary: MINPP1 is an enzyme responsible for metabolizing inositol hexakisphosphate and inositol 1,3,4,5,6 pentakisphosphate in mammalian cells. Our study shows that cytosolic expression of MINPP1 reduces cell growth and lowers the concentration of phosphatidylinositol 3,4,5-trisphosphate. These findings highlight the importance of MINPP1's confinement to the endoplasmic reticulum.
Article
Biochemistry & Molecular Biology
Maiwase Tembo, Rachel E. Bainbridge, Crystal Lara-Santos, Kayla M. Komondor, Grant J. Daskivich, Jacob D. Durrant, Joel C. Rosenbaum, Anne E. Carlson
Summary: This study investigates the interaction between TMEM16A channel and the membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2). The findings show that only lipids containing a phosphate at the 40 position effectively recover TMEM16A currents. These findings improve our understanding of how PI(4,5)P-2 binds to and potentiates TMEM16A channels.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biology
Ana Bura, Antonija Jurak Begonja
Summary: Phosphoinositides play crucial roles in cells with limited understanding of their localization and functions in platelets. This study investigated the localization of PI(4,5)P-2 and PI4P in resting and activated platelets through antibody staining. The intracellular pools of PI(4,5)P-2 and PI4P in platelets can be modulated by inhibitors of OCRL phosphatase and PI4KIII alpha kinase, with a more sensitive response in activated platelets.
Review
Cell Biology
Ana Bura, Sara Cabrijan, Iris Duric, Tea Bruketa, Antonija Jurak Begonja
Summary: Phosphoinositides (PIs) are phosphorylated lipids that have diverse cellular functions including regulation of trafficking, actin reorganization, and cell signaling. The two most abundant PIs in cells are phosphatidylinositol4-monophosphate (PI4P) and phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], which localize to different cellular compartments and regulate various processes. The levels of PIs are controlled by multiple kinases and phosphatases, and understanding their localization and function is important for studying cellular processes.
Article
Cell Biology
Muhmmad Omar-Hmeadi, Alenka Gucek, Sebastian Barg
Summary: Phosphatidylinositol(4,5)bisphosphate (PI(4,5)P2) is a crucial phospholipid in regulated exocytosis and some forms of endocytosis. The accumulation of PI(4,5)P2 at the release site inhibits fusion pore expansion and cargo release through an unconventional endocytic mechanism. This process involves the recruitment of endocytic fission machinery and is independent of clathrin-mediated endocytosis.
Article
Cell Biology
Martin Sztacho, Barbora Salovska, Jakub Cervenka, Can Balaban, Peter Hoboth, Pavel Hozak
Summary: Analysis of nuclear protein interactions with phosphoinositides reveals the role of PIP2 in regulating gene expression, RNA splicing, and cell cycle processes. The identified proteins are involved in various functions within the nucleus, with different PIP2-binding motifs influencing their localization and activity. This study provides insights into the molecular mechanism of nuclear PIP2 protein interaction and offers a methodology for further research on PIPs and other protein ligands.
Article
Biochemistry & Molecular Biology
Li Zhang, Lei Li, Ziqing Wei, Hao Zhou, Haowen Liu, Shen Wang, Yijing Ren, Tiankai Dai, Jiafan Wang, Zhitao Hu, Cong Ma
Summary: This study reveals the crystal structure of the CAPS-1 C2PH module, showing a tight interaction between the C2 and PH domains and enhanced binding to PI(4,5)P2 membrane. A novel PI(4,5)P2-binding site is identified in the C2 domain. Disruption of the C2-PH interaction or the PIP2-binding sites impairs CAPS-1 function in Ca2+-regulated exocytosis.
Review
Biochemistry & Molecular Biology
Kaori Kanemaru, Yoshikazu Nakamura
Summary: Phospholipase C (PLC) metabolizes phosphatidylinositol 4,5-bisphosphate in the plasma membrane and generates inositol 1,4,5-trisphosphate and diacylglycerol, which regulate cellular functions. Mammalian PLC consists of six typical classes and one atypical class, and dysregulation of PLC activity is associated with various diseases. Therefore, the review focuses on the structure, activation mechanisms, and physiological functions of mammalian PLC.
Article
Biochemistry & Molecular Biology
Mahmoud Suliman, Kendall C. Case, Michael W. Schmidtke, Pablo Lazcano, Chisom J. Onu, Miriam L. Greenberg
Summary: Inositol plays a significant role in cellular function and signaling. Its deficiency can cause cell death and abnormalities in inositol metabolism are associated with human disorders. This study provides a comprehensive understanding of the effects of inositol deprivation on phospholipid metabolism and gene expression in human cells, revealing the essential role of inositol in maintaining cell viability and regulating cell signaling and metabolism.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Biology
Katerina Cizkova, Katerina Koubova, Zdenek Tauber
Summary: This study revealed that activators and inhibitors of PPAR alpha affect the PI3K/Akt/PTEN pathway in intestinal cell differentiation, highlighting their impact on brush border formation.
Article
Biochemistry & Molecular Biology
Yong Shi, Anne Berking, Timo Baade, Kyle R. Legate, Reinhard Fassler, Christof R. Hauck
Summary: Staphylococcus aureus invades host cells mainly through an integrin-dependent manner, with phosphatidylinositol-4,5-bisphosphate (PI-4,5-P-2) playing a significant role in regulating integrin-associated proteins. Local synthesis of PI-4,5-P-2 by a focal adhesion-associated lipid kinase is crucial for integrin-mediated internalization of S. aureus. Targeting active PI-4,5-P-2 phosphatase to the plasma membrane and specific ablation of talin and FAK-binding motif in PIP5KI gamma 90 can reduce bacterial invasion.
MOLECULAR MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Andreas Santamaria, Javier Carrascosa-Tejedor, Eduardo Guzman, Nathan R. Zaccai, Armando Maestro
Summary: This study experimentally investigates the molecular organization of phosphatidylinositol 4,5-bisphosphate (PIP2) and reveals its correlation with the formation of transient PIP2 clusters in both lateral and perpendicular directions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Yi Wen, Volker M. Vogt, Gerald W. Feigenson
Summary: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) is a crucial component located at the inner leaflet of the cell membrane, constituting only 1-2% of total membrane lipids. Its synthesis and turnover are spatially and temporally regulated. Various factors, such as multivalent cations and cellular proteins interacting with PI(4,5)P-2, contribute to its versatile and dynamic distribution within membranes.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Review
Endocrinology & Metabolism
Farzad Asadi, Savita Dhanvantari
Summary: This study focuses on the hyperglucagonemia in diabetic patients and the potential mechanisms of alpha cell dysfunction in pancreatic alpha cells, exploring how inhibition of glucagon secretion can be targeted for the treatment of diabetes mellitus.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Kristen E. Rohli, Cierra K. Boyer, Sandra E. Blom, Samuel B. Stephens
Summary: Pancreatic islet beta-cells have the ability to adapt their secretory program to match nutrient availability and peripheral insulin signaling cues. However, as Type 2 diabetes progresses, these adaptive mechanisms fail, leading to a decline in beta-cell function and impaired insulin secretion.
Article
Biochemistry & Molecular Biology
Nara Lee Chon, J. Ryan Osterberg, Jack Henderson, Hanif M. Khan, Nathalie Reuter, Jefferson D. Knight, Hai Lin
Article
Biochemistry & Molecular Biology
J. Ryan Osterberg, Nara Lee Chon, Arthur Boo, Favinn A. Maynard, Hai Lin, Jefferson D. Knight
Article
Biochemistry & Molecular Biology
Brian P. Ziemba, Jefferson D. Knight, Joseph J. Falke
Article
Biochemistry & Molecular Biology
Devin S. Brandt, Matthew D. Coffman, Joseph J. Falke, Jefferson D. Knight
Article
Biochemistry & Molecular Biology
Joseph K. Vasquez, Kan Chantranuvatana, Daniel T. Giardina, Matthew D. Coffman, Jefferson D. Knight
Article
Biochemistry & Molecular Biology
Brian P. Ziemba, Jianing Li, Kyle E. Landgraf, Jefferson D. Knight, Gregory A. Voth, Joseph J. Falke
Article
Biophysics
Jefferson D. Knight, Joseph J. Falke
BIOPHYSICAL JOURNAL
(2009)
Article
Biophysics
Jefferson D. Knight, Michael G. Lerner, Joan G. Marcano-Velazquez, Richard W. Pastor, Joseph J. Falke
BIOPHYSICAL JOURNAL
(2010)
Article
Biochemistry & Molecular Biology
Jefferson D. Knight, Jessica A. Williamson, Andrew D. Miranker
Article
Biochemistry & Molecular Biology
Jefferson D. Knight, James A. Hebda, Andrew D. Miranker
Article
Biochemistry & Molecular Biology
JD Knight, AD Miranker
JOURNAL OF MOLECULAR BIOLOGY
(2004)
Article
Pharmacology & Pharmacy
JD Knight, RC Adami
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2003)
Article
Biochemistry & Molecular Biology
CM Eakin, JD Knight, CJ Morgan, MA Gelfand, AD Miranker
Article
Biochemistry & Molecular Biology
Raja Narayanasamy, Dandamudi Usharani, Ram Rajasekharan
Summary: This study investigated the role of ABHD16B in lipid metabolism. The overexpression of ABHD16B was found to decrease cellular triacylglycerol levels and increase phospholipid synthesis in yeast cells. Additionally, ABHD16B overexpression led to a reduction in lipid droplets and significant modifications in fatty acid composition. These findings highlight the importance of ABHD16B in lipid homeostasis and provide insights into its regulatory function in cellular lipid metabolism.
CHEMISTRY AND PHYSICS OF LIPIDS
(2024)