Journal
CHEMISTRY AND PHYSICS OF LIPIDS
Volume 165, Issue 4, Pages 475-481Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2011.11.013
Keywords
Phospholipid; Bilayer; Lamellarity; Lysis tension; Encapsulation efficiency; Dynamic light scattering
Categories
Funding
- Department of Science and Technology, India
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Unilamellar vesicle populations having a narrow size distribution and mean radius below 100 nm are preferred for drug delivery applications. In the present work, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was used to prepare giant unilamellar vesicles (GUVs) by electroformation and multilamellar vesicles (MLVs) by thin film hydration. Our experiments show that in contrast to MLVs, a single-pass extrusion of GUVs through track-etched polycarbonate membranes at moderate pressure differences is sufficient to produce small liposomes having low polydispersity index. Moreover, we observe that the drug encapsulating potential of extruded liposomes obtained from GUVs is significantly higher compared to liposomes prepared by extrusion of MLVs. Furthermore, our experiments carried out for varying membrane pore diameters and extrusion pressures suggest that the size of extruded liposomes is a function of the velocity of GUV suspensions in the membrane pore. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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