Journal
CHEMISTRY AND PHYSICS OF LIPIDS
Volume 163, Issue 6, Pages 552-560Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2010.04.005
Keywords
Detection limit; Di-FCCA; MALDI-TOF MS; Matrices; Phosphatidylethanolamine; Phosphatidylcholine; Triacylglycerol
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Funding
- Leipzig Interdisciplinary Research Cluster of Genetic Factors, Clinical Phenotypes and Environment (LIFE Center, University of Leipzig)
- European Union
- European Regional Development Fund (ERFD)
- Free State of Saxony
- German Research Council (DFG) [Transregio 67/A2, FU 771/1-1]
- LTH bank for infrastructure
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Due to its sensitivity, the tolerance of impurities and the simplicity of performance, matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is increasingly used to analyze lipids from biological sources. Although its detailed role is not understood so far, the applied matrix has a pronounced effect on the achievable spectrum quality and particularly how sensitive the individual lipid classes are detectable. Different matrix compounds were recently established in the lipid field including 2,5-dihydroxybenzoic acid (DHB), 9-aminoacridine (9-AA), para-nitroaniline (PNA), 2-mercaptobenzothiazole (MBT), and 2-(2-aminoethylamino)-5-nitropyridine (AAN). It is the aim of this paper to compare the properties of these matrices with the newly synthesized matrix, alpha-cyano-2,4-difluorocinnamic acid (Di-FCCA). An organic extract from hen egg yolk was used as a simple and easily available test system. It will be shown that Di-FCCA is the matrix of choice to detect lipids in the positive-ion mode due to an achievable sensitivity gain of more than one order of magnitude compared to alternative matrices. In contrast, Di-FCCA is not suitable for negative-ion detection of phospholipids. Here, 9-AA is unequivocally the matrix of choice. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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