Journal
CHEMICO-BIOLOGICAL INTERACTIONS
Volume 220, Issue -, Pages 208-213Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2014.07.002
Keywords
CdSe nanoparticles; Saccharomyces cerevisiae; Nanotoxicity; Vacuolar membrane permeabilization
Funding
- National Natural Science Foundation of China [81373039, 8117154, 21271108]
- Natural Science Foundation of Tianjin [13JCYBJC20700, 12HZGJHZ01100]
- Foundation of National Basic Science personnel training [J1103503]
Ask authors/readers for more resources
Cadmium selenide (CdSe) nanoparticles are implemented in a wide range of applications, but their potential risk to the ecosystem, especially to the organisms essential for the maintenance of ecosystem homeostasis, such as fungal populations, plants and bacteria, remains to be elucidated. In this study, we investigated their toxicity to one of the most important fungal model organisms, Saccharomyces cerevisiae. Growth inhibition assays revealed that the synthesized CdSe nanoparticles with the sizes of 20-30 nm had strong inhibitory effect on yeast growth (IC50 = 80 ppm). This toxicity was not attributed to mitochondrial dysfunction and autophagy, but was dependent on End3-mediated endocytosis, and was associated with reactive oxygen species (ROS) accumulation and an enhancement of vacuolar membrane permeabilization (VMP). These results reveal a key role of the vacuole during the interaction between CdSe nanoparticles and yeast cells. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available