Article
Toxicology
Yoshio Nakagawa, Jin Suzuki, Toshinari Suzuki, Hideyo Takahashi, Kosho Makino, Yasushi Ono, Miho Sakamoto, Akiko Inomata
Summary: The abuse of novel psychoactive compounds derived from the analgesic drug fentanyl has caused numerous fatalities. In this study, the cytotoxic effects and mechanisms of 4F-iBF, 4Cl-iBF, and iBF were investigated in rat hepatocytes. 4F-iBF caused cell death, depletion of cellular ATP and glutathione levels, and accumulation of oxidized glutathione. The cytotoxicity induced by 4Cl-iBF and 4F-iBF was greater than that induced by iBF, involving mitochondrial membrane potential loss and production of reactive oxygen species. These cytotoxic effects were partially alleviated by N-acetyl-l-cysteine, a precursor of cellular glutathione, while exacerbated by diethyl maleate, a glutathione depletor.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Polymer Science
Hongcai Wang, Xiuqiong Chen, Yanshi Wen, Dongze Li, Xiuying Sun, Zhaowen Liu, Huiqiong Yan, Qiang Lin
Summary: In this study, oxidized sodium alginate (OSA) with varying degrees of oxidation was successfully synthesized using NaIO4 as the oxidant. The results showed that as the oxidation degree increased, the molecular weight of OSA decreased, the gelation ability weakened, and the degradation performance improved. This research is of great significance for expanding the applications of OSA-based hydrogel in tissue engineering, controlled drug release, 3D printing, and the biomedical field.
Article
Multidisciplinary Sciences
Juliana C. Correa-Velloso, Paula J. Bartlett, Robert Brumer, Lawrence D. Gaspers, Henning Ulrich, Andrew P. Thomas
Summary: Rat hepatocytes express multiple purinergic P2Y and P2X receptors, leading to distinct Ca2+ oscillations. These oscillation patterns are shaped by protein kinase C, resulting in unique cellular responses to different agonists.
Article
Biochemistry & Molecular Biology
Michal Glensk, Marta K. Dudek, Peter Kinkade, Evelyn C. S. Santos, Vitold B. Glinski, Daneel Ferreira, Ewa Seweryn, Slawomir Kazmierski, Joao B. Calixto, Jan A. Glinski
Summary: This study discovered the presence of echihumiline and hydroxymyoscorpine in Echium plantagineum for the first time. It also revealed that under standard HPLC acidic conditions, echimidine co-elutes with its isomers, potentially confusing the true alkaloidal composition.
Article
Biochemistry & Molecular Biology
Jelena Markovic Filipovic, Marko Miler, Danijela Kojic, Jelena Karan, Ivana Ivelja, Jovana Cukuranovic Kokoris, Milica Matavulj
Summary: The treatment of Acrylamide can affect protein expression and cellular redox status in the liver, leading to changes in cellular metabolic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Heloisa C. de Moura, Claudio R. Novello, Evellin Balbinot-Alfaro, Elisangela Dusman, Helyn P. O. Barddal, Igor V. Almeida, Veronica E. P. Vicentini, Carlos Prentice-Hern Hernandez, Alexandre T. Alfaro
Summary: This study revealed that tilapia scales are a potential source for obtaining chondroitin sulfate, which showed anticoagulant and cytotoxic/antitumor activities.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Physical
Ruitao Wu, Kaitlyn R. Wiegand, Lichang Wang
Summary: This study focuses on the C-C bond cleavage of ethanol on Ir(100), using density functional theory calculations and the degree of dehydrogenation of reactant species to correlate the cleavage barrier and reaction energy. The results show that the cleavage barrier generally decreases with decreasing degree of dehydrogenation, with a local minimum around a specific degree. Additionally, oxygen atom in the reactive species plays a critical role in lowering the C-C bond cleavage barrier.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Mathematics, Applied
Karla Ferjancic, Marjeta Knez, Vito Vitrih
Summary: This paper presents two methods for constructing a C-2 rational spline motion of degree six, one requiring the solution of a global linear system of equations and the other splitting the construction into local biarc segments. Both methods involve the selection of additional free parameters to ensure uniqueness of the solution, along with examination and remedies for remaining free parameters in the rotational part. Various possibilities for constructing the translational part are also proposed in this study.
JOURNAL OF COMPUTATIONAL AND APPLIED MATHEMATICS
(2021)
Article
Materials Science, Multidisciplinary
R. Malacarne, S. Mathieu, M. Siblani, L. Aranda, S. Knittel, C. Desgranges, M. Vilasi
Summary: MxOy-Na2SO4 (M = Ni, Cr, Fe, Al, Ti, Nb) and NiSO4-Na2SO4 mixtures were prepared and their reactivity at 650 degrees C in air + SO2 (400 and 1000 ppm) was investigated. It was found that a molten phase was formed in NiSO4-Na2SO4 mixtures, and rapid sulfation of NiO occurred under these conditions. The sulfation of NiO should be considered in explaining Type II hot corrosion attacks. Cr2O3, Fe2O3, and Al2O3 also reacted with the gas phase, indicating dissolution of these oxides through an acidic-fluxing mechanism in air + SO2/SO3 (400 and 1000 ppm) environments. Al2O3 exhibited higher solubility than Cr2O3 under these conditions.
Review
Food Science & Technology
Zhipeng Lin, Jian Wei, Yongqiang Hu, Dujuan Pi, Mingguo Jiang, Tao Lang
Summary: Caffeine, derived from purine nucleotides, constitutes 2-5% of dry weight in tea and 1-2% in coffee. While plants use methylation reactions and nucleosidase reactions, bacterial degradation occurs through N-demethylation and C-8 oxidation pathways. This study aims to comprehensively summarize the metabolism and application of caffeine in microorganisms, shedding light on its potential prospects.
Article
Biochemistry & Molecular Biology
Hubert Zywno, Wiktor Bzdega, Adrian Kolakowski, Piotr Kurzyna, Ewa Harasim-Symbor, Klaudia Sztolsztener, Adrian Chabowski, Karolina Konstantynowicz-Nowicka
Summary: Coumestrol significantly reduces the accumulation of sphingolipids in insulin-resistant hepatocytes and promotes the expression of fatty acid transport proteins, improving hepatic insulin resistance. Additionally, Coumestrol alters the sphingolipid salvage pathway, leading to excessive sphingolipid deposition.
Article
Biochemistry & Molecular Biology
Jiachang Liu, Xiaoyan Liu, Jie Fu, Bingya Jiang, Shufen Li, Linzhuan Wu
Summary: Isatropolones/isarubrolones are secondary metabolites produced by actinomycetes, characterized by the presence of a tropolone ring in their structures. A study on the producer strain Streptomyces sp. CPCC 204095 led to the discovery of 7,12-dihydroisatropolone C (H2ITC) as a major metabolite during the mycelial growth period. H2ITC can spontaneously oxidize into isatropolone C (ITC), indicating that H2ITC serves as the physiological precursor of ITC. The characterization of H2ITC suggests the involvement of dihydrotropolone-ring construction in the biosynthesis of isatropolones.
Article
Materials Science, Multidisciplinary
Xiaowen Yang, Lanlan Yang, Jinlong Wang, Zehao Chen, Minghui Chen, Jianqiang Zhang, Fuhui Wang
Summary: This study investigates the hot corrosion behavior of N5 alloy in molten sulfate at 850°C after different peroxidation treatments. Preoxidation was performed at 900, 1000, and 1100°C for 20 and 100 hours, respectively. The preformed oxide scale had a multilayered structure and varying amounts of Ta oxides. Among all the samples, those preoxidized at 900°C exhibited the highest corrosion resistance. However, a porous structure with internal oxides and sulfides was observed when preoxidized at 1000 and 1100°C. The alloy without preoxidation directly reacted with the molten salt to form a porous structure with internal oxides and sulfides.
Article
Green & Sustainable Science & Technology
Le Yang, Hongju Lin, Zhihao Fang, Yanhui Yang, Xiaohao Liu, Gangfeng Ouyang
Summary: Developing a direct conversion route of methane toward oxygenates under milder conditions has significant economic value and potential. This review summarizes the efforts in developing selective thermo-catalytic oxidation of methane to oxygenates, mainly methanol, in the past decade. The study reveals the active site configurations and catalytic mechanisms with different oxidants, such as O2, H2O2, N2O, and H2O. The review also presents perspectives on catalyst design and process innovation.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Article
Materials Science, Multidisciplinary
Bo Meng, Jinlong Wang, Minghui Chen, Shenglong Zhu, Fuhui Wang
Summary: A novel thermal barrier coating system with a double columnar structure was developed to investigate its cyclic oxidation behavior. The system does not experience elemental interdiffusion during high-temperature exposure and the high grain boundary density in the bond coating promotes the selective oxidation of Al, improving oxidation resistance and rumpling resistance of the thermal barrier coating.
Article
Nanoscience & Nanotechnology
Ana Vieira, Laura Rodriguez-Lorenzo, Isabel B. Leonor, Rui L. Reis, Begona Espina, Marilia Barreiros dos Santos
Summary: This study demonstrates an innovative approach to enhance antibacterial surfaces by incorporating titanium dioxide nanoparticles into microstructured surfaces. The engineered surfaces effectively reduced bacterial colonization through a combination of UVC disinfection and TiO2 photocatalysis. The results suggest that this synergistic strategy holds great potential for various applications in healthcare and biotechnology markets.
ACS APPLIED BIO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Siva Nemala Sankar, Joao Fernandes, Marilia Barreiros dos Santos, Begona Espina, Pedro Alpuim, Ander Garcia Diez, Senentxu Lanceros-Mendez, Lalita Saini, Suvigya Kaushik, Gopinadhan Kalon, Andrea Capasso
Summary: In this study, an efficient and green strategy was proposed to fabricate mu m-thick, graphene-based laminates by liquid phase exfoliation and vacuum filtration. The membranes showed structural and mechanical stability during long-term operation in water, and exhibited size selection and anion-selectivity in ion transport studies. Additionally, the membranes demonstrated high rejection rates and antibacterial properties. Therefore, these green graphene-based membranes offer a viable option for future water management applications.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemical Research Methods
Cristian Suarez-Oubina, Paloma Herbello-Hermelo, Natalia Mallo, Maria Vazquez, Santiago Cabaleiro, Ivone Pinheiro, Laura Rodriguez-Lorenzo, Begona Espina, Pilar Bermejo-Barrera, Antonio Moreda-Pineiro
Summary: This research focuses on using single-cell inductively coupled plasma-mass spectrometry (scICP-MS) to assess the associations of titanium dioxide nanoparticles (TiO2 NP) and silver nanoparticles (Ag NP) in cell lines derived from aquaculture species. The optimization studies aim to avoid high dissolved background, multi-cell peak coincidence, and possible spectral interferences. The results show that TiO2 NPs mainly interact with the outer cell membranes, while Ag NPs can enter the cells.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Jessica Caldwell, Celine Loussert-Fonta, Gaelle Toullec, Niclas Heidelberg Lyndby, Beat Haenni, Patricia Taladriz-Blanco, Begona Espina, Barbara Rothen-Rutishauser, Alke Petri-Fink
Summary: Many researchers are studying the interaction between microplastics and marine fauna, aiming to understand exposure pathways and concentrations, as well as the impact of such interactions. This study focused on how Cassiopea andromeda jellyfish interacted with microplastics, using different analytical techniques. The results showed that the fluorescent microplastics could be successfully detected and their interaction with the jellyfish was likely influenced by the properties of the microplastics.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sofia Neto, Ana Vieira, Hugo Oliveira, Begona Espina
Summary: This study successfully established an A. baumannii infection model using tail-injured zebrafish embryos. The model was able to distinguish the most virulent strains from the less virulent ones. Furthermore, using phage therapy, the infection of the most virulent strains was controlled, leading to increased survival rates.
Article
Engineering, Environmental
Tianxing Wang, Soraia P. S. Fernandes, Joana Araujo, Xiaoxi Li, Laura M. Salonen, Begona Espina
Summary: In this study, a covalent organic polymer was designed and synthesized for the efficient adsorption of STX. The prepared material showed fast adsorption of STX within 1 hour and demonstrated good reusability and high recovery rates, making it a potential candidate for the extraction and pre-concentration of STX from aquatic environments.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Elza Fonseca, Maria Vazquez, Laura Rodriguez-Lorenzo, Natalia Mallo, Ivone Pinheiro, Maria Ligia Sousa, Santiago Cabaleiro, Monica Quarato, Miguel Spuch-Calvar, Miguel A. Correa-Duarte, Juan Jose Lopez-Mayan, Mick Mackey, Antonio Moreda, Vitor Vasconcelos, Begona Espina, Alexandre Campos, Mario Jorge Araujo
Summary: In this study, the effects of citrate-coated TiO2 nanoparticles (TiO2 NPs) of two different primary sizes on the liver of flatfish turbot were investigated. The results showed that the abundance of lipid droplets in hepatocytes varied depending on the size of TiO2 NPs, with an increase in turbot exposed to smaller NPs and a depletion with larger NPs. Gene expression related to oxidative and immune responses as well as lipid metabolism was also influenced by TiO2 NPs. These findings emphasize the importance of scrutinizing the risks associated with exposure to NPs with distinct properties in aquatic organisms.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Chemistry, Analytical
Juan Jose Lopez-Mayan, Blanca Alvarez-Fernandez, Elena Pena-Vazquez, Maria Carmen Barciela-Alonso, Antonio Moreda-Pineiro, Julie Maguire, Mick Mackey, Monica Quarato, Ivone Pinheiro, Begona Espina, Laura Rodriguez-Lorenzo, Pilar Bermejo-Barrera
Summary: A study was conducted to investigate the bioaccumulation of citrate-coated titanium dioxide nanoparticles in red (Palmaria palmata) and green (Ulva sp.) seaweed. The results showed that Ulva sp. had higher titanium concentrations than Palmaria palmata under the same exposure conditions. The titanium concentration in Ulva sp. reached its maximum after 28 days of exposure to 1.0 mgL(-1) of 5 nm TiO(2)NPs. The sizes and concentrations of TiO(2)NPs determined by SP-ICP-MS were similar for both seaweeds, suggesting that the titanium is mainly accumulated in Ulva sp. as ionic titanium or nanoparticles smaller than the limit of detection in size.
Article
Chemistry, Multidisciplinary
Ivone Pinheiro, Monica Quarato, Antonio Moreda-Pineiro, Ana Vieira, Virginie Serin, David Neumeyer, Nicolas Ratel-Ramond, Sebastien Joulie, Alain Claverie, Miguel Spuch-Calvar, Miguel A. Correa-Duarte, Alexandre Campos, Jose Carlos Martins, Pilar Bermejo-Barrera, Marisa P. Sarria, Laura Rodriguez-Lorenzo, Begona Espina
Summary: Commercial ATO nanoparticles are relatively harmless to zebrafish and marine mussels, but they do affect the developmental parameters of zebrafish. Further research is needed on the accumulation of nanoparticles in the human body.
Article
Chemistry, Multidisciplinary
Vanesa Romero, Soraia P. S. Fernandes, Liliana P. L. Goncalves, Orlando Oliveira, Maria Meledina, Karol Strutynski, Manuel Melle-Franco, Yury V. Kolen'ko, Begona Espina, Laura M. Salonen
Summary: A one-step procedure was reported for synthesizing a crystalline magnetic covalent organic framework (COF) composite, which involved the use of a sub-stoichiometric amount of a diamino building block during the COF synthesis. The resulting composite showed no losses in crystallinity or surface area compared to bulk material, and exhibited efficient adsorption of okadaic acid in synthetic seawater. Furthermore, the composite material could be recycled with minor losses in adsorption capacity, presenting a promising approach for cost-effective and efficient seawater biotoxin monitoring using COF composites.
Article
Engineering, Environmental
Jessica Caldwell, Alke Petri-Fink, Celine Loussert-Fonta, Gaelle Toullec, Niclas Heidelberg Lyndby, Beat Haenni, Patricia Taladriz-Blanco, Barbara Rothen-Rutishauser
Summary: Many researchers are studying the interaction between microplastics and marine fauna and working to monitor exposure pathways, concentrations, and their impacts. This study focuses on Cassiopea andromeda jellyfish, which live in (sub-)tropical coastal regions that are potentially contaminated by plastic waste. Fluorescent microplastics were detected using an optimized analytical protocol, and their interaction with the jellyfish was found to be influenced by the properties of the microplastics, such as density and hydrophobicity.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Food Science & Technology
Celia Costas, M. Carmen Louzao, Sandra Raposo-Garcia, Carmen Vale, Almudena Grana, a Cristina Carrera, Jose Manuel Cifuentes, Natalia Vilarino, Mercedes R. Vieytes, Luis M. Botana
Summary: This study assessed the oral toxicity and intraperitoneal toxicity of Brevetoxin 3 (PbTx3) in mice, and found that oral administration resulted in lower toxicity. The results of this study are important for evaluating the risks of PbTxs to human health.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Chemistry, Medicinal
Sandra Raposo-Garcia, Alejandro Cao, Celia Costas, M. Carmen Louzao, Natalia Vilarino, Carmen Vale, Luis M. Botana
Summary: The expanding microorganisms producing ciguatoxins have raised significant concerns about ciguatera fish poisoning (CF), necessitating the development of a reliable and rapid ciguatoxin detection method to ensure food safety. This study evaluated various cell lines for CTX detection and revealed the limitations of the N2a cell line due to low availability of sodium channels and significant O/V damage. The HEK293 Nav cell line, expressing the alpha 1.6 subunit of sodium channels, proved to be sensitive to ciguatoxin without sensitization with O/V, providing an alternative for sodium channel-targeting compound detection.
Article
Chemistry, Analytical
L. G. Valle, B. Santamaria, A. Lavin, M. F. Laguna, L. Rodriguez-Lorenzo, B. Espina, M. Holgado
Summary: In this study, we report the development of a biosensing system based on the Interferometric Optical Detection Method (IODM), using gold nanoparticles as interferometric enhancers to improve the performance of immunoassays. Through experiments and calculations, we demonstrate that the gold nanoparticles act as amplifiers in the IODM and have the potential to be applied in various biological detection applications.