Article
Chemistry, Analytical
Jingyang Zhang, Yu Wang, Xudong Zhao, Mengmeng Chen, Yuan Peng, Jialei Bai, Shuang Li, Dianpeng Han, Shuyue Ren, Kang Qin, Sen Li, Tie Han, Zhixian Gao
Summary: The development of a dual sensitization smartphone colorimetric strategy based on rolling circle amplification (RCA) coils gathering Au tetrahedra has been successful. This strategy utilizes the dual sensitization effect of RCA and Au tetrahedra to amplify the signal. The use of ImageJ software allows for timely target detection under practical conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chen-Chen Li, Jinping Hu, Xiliang Luo, Juan Hu, Chun-Yang Zhang
Summary: A quantum dot-mediated fluorescence nanosensor was developed for sensitive detection of SNPs in cancer cells, offering high amplification efficiency, zero background signal, good specificity, and high sensitivity. It can detect a wide range of SNPs and accurately identify mutations as low as 0.001% in mixtures, achieving single-cell level SNP quantification.
ANALYTICAL CHEMISTRY
(2021)
Article
Biology
Linfeng Cao, Ziwen Meng, Junjie Tan, Ming Ying, Meiying Bi, Yanjun Liu, Xinrui Tong, Jiaxun Wei, Lei Huang
Summary: This study developed DNA nanoparticles (citZ-boxes) for automatic loading and releasing of cargo. Using restriction enzyme cleavage, the enhanced green fluorescent protein (eGFP) gene was delivered into B. subtilis protoplasts, resulting in eGFP expression.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaowen Cao, Feng Chen, Jing Xue, Yue Zhao, Min Bai, Yongxi Zhao
Summary: Here, we present a hierarchical DNA branch assembly-encoded fluorescent nanoladders for denoised and highly multiplexed signal amplification of single-cell transcripts. Our method achieves independent RNA-primed rolling circle amplification without nonspecific amplification and allows for programmable DNA branch assembly to encode virtual signals for visualizing numbers of targets. We demonstrate accurate RNA profiling in different cancer cells and reveal diverse localization patterns for spatial regulation of transcripts.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Ruolan Huang, Kun Liu, Chensirong Yue, Ying Wang, Zhilong Zeng, Lu Xiao, Lei Dong, Rongqin Ke, Chaoyong Yang, Dan Liu
Summary: This study developed a method for visualizing mRNA at single-molecule resolution using rolling circle amplification and streptavidin-modified alkaline phosphatase (SA-ALP) detection. The method provides highly selective and sensitive RNA detection, comparable to fluorescence-based assays. It also overcomes common background interference issues and allows for the quantification of mRNA expression from two genes in single cells and multiple cell types.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xinfu Zhang, Sandra Garrett, Brenton R. Graveley, Michael P. Terns
Summary: This study characterized the uptake of CRISPR spacers by type III CRISPR-Cas systems within their native host, Streptococcus thermophilus. The results showed that Cas1 and Cas2 proteins are critical for type III adaptation, while genes responsible for crRNA biogenesis or interference do not significantly affect spacer uptake patterns. Type III spacers are acquired in a PAM- and orientation-independent manner, and certain regions of plasmids and the host genome are preferentially sampled during type III adaptation. Additionally, the type III system can adapt to and protect host cells from lytic phage infection.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemical Research Methods
Chi Yao, Rui Zhang, Jianpu Tang, Dayong Yang
Summary: DNA hydrogels have unique properties and can be synthesized efficiently using rolling circle amplification.
Article
Chemistry, Analytical
Yanheng Yao, Chengjie Duan, Yan Chen, Zhiqiang Hou, Wenting Cheng, Dayong Li, Zhongyun Wang, Yang Xiang
Summary: A fluorescent biosensor for detecting lncRNA HULC was developed based on rolling circle amplification induced by multi-primer probes. The method showed high selectivity and sensitivity, providing a new approach for translating specific lncRNA into clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Changping Yang, Xiaohui Wu, Jianbing Liu, Baoquan Ding
Summary: Nucleic acid molecules, as engineerable building blocks, can be designed to form versatile nanostructures with unique shapes and sizes. These nanostructures, which are programmable and biocompatible, have been widely used in biomedical applications. Stimuli-responsive nucleic acid nanostructures have been constructed through tailored chemical modification, allowing drug delivery in response to various stimuli. This minireview summarizes the representative and latest research on stimuli-responsive nucleic acid nanostructures for drug delivery and discusses their prospects and challenges in biomedical applications.
Article
Chemistry, Multidisciplinary
Pravin Pokhrel, Sagun Jonchhe, Wei Pan, Hanbin Mao
Summary: Interaction between peptides and nucleic acids plays a crucial role in cellular functions. Recent studies have shown that this interaction is involved in liquid-liquid phase separation (LLPS) and the formation of membraneless organelles. In this study, the molecular interaction between poly-l-lysine (PLL) peptide and nucleic acids during the early stage of LLPS was investigated at the single-molecule level. The results revealed a multistage LLPS process mediated by long-range interactions between nucleic acids and polyelectrolytes. The propensity to form rigid nucleic acid-PLL complexes was found to reduce condensate formation during LLPS.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biology
Emil Laust Kristoffersen, Matthew Burman, Agnes Noy, Philipp Holliger
Summary: RNA-catalyzed RNA replication is a crucial step in the emergence of life's first genetic system. This study explores rolling circle synthesis as a potential solution to the problem of strand separation. The results demonstrate that RNA can catalyze all steps of viroid-like RNA replication, and molecular dynamics simulations provide insights into the potential mechanism of rolling circle synthesis.
Article
Biophysics
Sidan Wang, Haojia Li, Kejun Dong, Wan Shu, Jiarui Zhang, Jun Zhang, Rong Zhao, Sitian Wei, Dilu Feng, Xianjin Xiao, Wei Zhang
Summary: Point of care testing (POCT) plays a crucial role in disease diagnosis and prognosis evaluation. The biosensor based on CRISPR/Cas12a is a powerful diagnostic tool due to its high sensitivity. However, its limited recognition ability and high cost hinder its universal application. Therefore, it is important to develop a CRISPR/Cas12a-based RNA detection system that is easy to use, cheap, specific, and universal.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Weiliang Liu, Fengxia Su, Kejian Gao, Desheng Chen, Zhengping Li
Summary: We have developed a method to quantify N-6-methyladenosine with high resolution using double blocking gap-filling-ligation and cascade isothermal amplification. This method can detect as low as 1 fM target RNA and achieve a selectivity of approximately 100-fold between m(6)A and A. It has been successfully applied to analyze m(6)A at specific sites in cell samples.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Jianpu Tang, Aiqi Liang, Chi Yao, Dayong Yang
Summary: The review article by Yang, Yao and colleagues discusses recent advancements in biofunctional DNA hydrogels and DNA nanocomplexes based on rolling circle amplification (RCA), and introduces assembly strategies and functionalization methods of ultralong single-strand DNA produced by RCA.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)